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Protein

Spermidine/putrescine-binding periplasmic protein

Gene

potD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36Spermidine1 Publication1
Binding sitei85Spermidine1 Publication1
Binding sitei327Spermidine1 Publication1

GO - Molecular functioni

  • putrescine binding Source: EcoCyc
  • spermidine binding Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
  • spermidine transport Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:POTD-MONOMER
MetaCyc:POTD-MONOMER

Protein family/group databases

TCDBi3.A.1.11.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine-binding periplasmic protein
Short name:
SPBP
Gene namesi
Name:potD
Ordered Locus Names:b1123, JW1109
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10752 potD

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03566 Spermidine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 233 PublicationsAdd BLAST23
ChainiPRO_000003183724 – 348Spermidine/putrescine-binding periplasmic proteinAdd BLAST325

Proteomic databases

EPDiP0AFK9
PaxDbiP0AFK9
PRIDEiP0AFK9

2D gel databases

SWISS-2DPAGEiP0AFK9

Interactioni

Protein-protein interaction databases

BioGridi426179757 interactors.
IntActiP0AFK9 7 interactors.
STRINGi316385.ECDH10B_1195

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 38Combined sources12
Helixi42 – 50Combined sources9
Beta strandi52 – 60Combined sources9
Helixi62 – 69Combined sources8
Beta strandi73 – 75Combined sources3
Beta strandi77 – 80Combined sources4
Helixi84 – 86Combined sources3
Helixi87 – 93Combined sources7
Helixi101 – 103Combined sources3
Helixi107 – 109Combined sources3
Helixi112 – 114Combined sources3
Beta strandi127 – 139Combined sources13
Turni140 – 142Combined sources3
Turni145 – 147Combined sources3
Helixi152 – 155Combined sources4
Helixi157 – 159Combined sources3
Beta strandi163 – 165Combined sources3
Helixi169 – 179Combined sources11
Helixi189 – 201Combined sources13
Helixi203 – 205Combined sources3
Beta strandi206 – 209Combined sources4
Helixi215 – 219Combined sources5
Beta strandi224 – 229Combined sources6
Helixi230 – 237Combined sources8
Beta strandi243 – 246Combined sources4
Beta strandi253 – 260Combined sources8
Helixi268 – 279Combined sources12
Helixi281 – 291Combined sources11
Beta strandi296 – 298Combined sources3
Turni299 – 303Combined sources5
Helixi306 – 309Combined sources4
Turni312 – 314Combined sources3
Helixi318 – 323Combined sources6
Beta strandi324 – 326Combined sources3
Helixi331 – 333Combined sources3
Helixi334 – 344Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POTX-ray1.80A24-348[»]
1POYX-ray2.501/2/3/426-348[»]
ProteinModelPortaliP0AFK9
SMRiP0AFK9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFK9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 171Spermidine-binding1 Publication4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107RUE Bacteria
COG0687 LUCA
HOGENOMiHOG000263814
InParanoidiP0AFK9
KOiK11069
OMAiIYEKYWS
PhylomeDBiP0AFK9

Family and domain databases

InterProiView protein in InterPro
IPR006059 SBP
IPR001188 Sperm_putr-bd
PfamiView protein in Pfam
PF13416 SBP_bac_8, 1 hit
PIRSFiPIRSF019574 Periplasmic_polyamine_BP, 1 hit
PRINTSiPR00909 SPERMDNBNDNG

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKWSRHLLA AGALALGMSA AHADDNNTLY FYNWTEYVPP GLLEQFTKET
60 70 80 90 100
GIKVIYSTYE SNETMYAKLK TYKDGAYDLV VPSTYYVDKM RKEGMIQKID
110 120 130 140 150
KSKLTNFSNL DPDMLNKPFD PNNDYSIPYI WGATAIGVNG DAVDPKSVTS
160 170 180 190 200
WADLWKPEYK GSLLLTDDAR EVFQMALRKL GYSGNTTDPK EIEAAYNELK
210 220 230 240 250
KLMPNVAAFN SDNPANPYME GEVNLGMIWN GSAFVARQAG TPIDVVWPKE
260 270 280 290 300
GGIFWMDSLA IPANAKNKEG ALKLINFLLR PDVAKQVAET IGYPTPNLAA
310 320 330 340
RKLLSPEVAN DKTLYPDAET IKNGEWQNDV GAASSIYEEY YQKLKAGR
Length:348
Mass (Da):38,867
Last modified:December 20, 2005 - v1
Checksum:i1E6EB7A6B4EC63AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA Translation: AAC37041.1
U00096 Genomic DNA Translation: AAC74207.1
AP009048 Genomic DNA Translation: BAA35943.1
PIRiD40840
RefSeqiNP_415641.1, NC_000913.3
WP_000759317.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74207; AAC74207; b1123
BAA35943; BAA35943; BAA35943
GeneIDi945682
KEGGiecj:JW1109
eco:b1123
PATRICifig|1411691.4.peg.1144

Similar proteinsi

Entry informationi

Entry nameiPOTD_ECOLI
AccessioniPrimary (citable) accession number: P0AFK9
Secondary accession number(s): P23861
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome