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Protein

Spermidine/putrescine-binding periplasmic protein

Gene

potD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36Spermidine1 Publication1
Binding sitei85Spermidine1 Publication1
Binding sitei327Spermidine1 Publication1

GO - Molecular functioni

  • polyamine binding Source: InterPro
  • polyamine-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • polyamine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTD-MONOMER.
ECOL316407:JW1109-MONOMER.
MetaCyc:POTD-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine-binding periplasmic protein
Short name:
SPBP
Gene namesi
Name:potD
Ordered Locus Names:b1123, JW1109
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10752. potD.

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 233 PublicationsAdd BLAST23
ChainiPRO_000003183724 – 348Spermidine/putrescine-binding periplasmic proteinAdd BLAST325

Proteomic databases

EPDiP0AFK9.
PaxDbiP0AFK9.
PRIDEiP0AFK9.

2D gel databases

SWISS-2DPAGEP0AFK9.

Interactioni

Protein-protein interaction databases

BioGridi4261797. 10 interactors.
IntActiP0AFK9. 7 interactors.
STRINGi511145.b1123.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 38Combined sources12
Helixi42 – 50Combined sources9
Beta strandi52 – 60Combined sources9
Helixi62 – 69Combined sources8
Beta strandi73 – 75Combined sources3
Beta strandi77 – 80Combined sources4
Helixi84 – 86Combined sources3
Helixi87 – 93Combined sources7
Helixi101 – 103Combined sources3
Helixi107 – 109Combined sources3
Helixi112 – 114Combined sources3
Beta strandi127 – 139Combined sources13
Turni140 – 142Combined sources3
Turni145 – 147Combined sources3
Helixi152 – 155Combined sources4
Helixi157 – 159Combined sources3
Beta strandi163 – 165Combined sources3
Helixi169 – 179Combined sources11
Helixi189 – 201Combined sources13
Helixi203 – 205Combined sources3
Beta strandi206 – 209Combined sources4
Helixi215 – 219Combined sources5
Beta strandi224 – 229Combined sources6
Helixi230 – 237Combined sources8
Beta strandi243 – 246Combined sources4
Beta strandi253 – 260Combined sources8
Helixi268 – 279Combined sources12
Helixi281 – 291Combined sources11
Beta strandi296 – 298Combined sources3
Turni299 – 303Combined sources5
Helixi306 – 309Combined sources4
Turni312 – 314Combined sources3
Helixi318 – 323Combined sources6
Beta strandi324 – 326Combined sources3
Helixi331 – 333Combined sources3
Helixi334 – 344Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POTX-ray1.80A24-348[»]
1POYX-ray2.501/2/3/426-348[»]
ProteinModelPortaliP0AFK9.
SMRiP0AFK9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFK9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 171Spermidine-binding1 Publication4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107RUE. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263814.
InParanoidiP0AFK9.
KOiK11069.
OMAiMDDSREV.
PhylomeDBiP0AFK9.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKWSRHLLA AGALALGMSA AHADDNNTLY FYNWTEYVPP GLLEQFTKET
60 70 80 90 100
GIKVIYSTYE SNETMYAKLK TYKDGAYDLV VPSTYYVDKM RKEGMIQKID
110 120 130 140 150
KSKLTNFSNL DPDMLNKPFD PNNDYSIPYI WGATAIGVNG DAVDPKSVTS
160 170 180 190 200
WADLWKPEYK GSLLLTDDAR EVFQMALRKL GYSGNTTDPK EIEAAYNELK
210 220 230 240 250
KLMPNVAAFN SDNPANPYME GEVNLGMIWN GSAFVARQAG TPIDVVWPKE
260 270 280 290 300
GGIFWMDSLA IPANAKNKEG ALKLINFLLR PDVAKQVAET IGYPTPNLAA
310 320 330 340
RKLLSPEVAN DKTLYPDAET IKNGEWQNDV GAASSIYEEY YQKLKAGR
Length:348
Mass (Da):38,867
Last modified:December 20, 2005 - v1
Checksum:i1E6EB7A6B4EC63AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37041.1.
U00096 Genomic DNA. Translation: AAC74207.1.
AP009048 Genomic DNA. Translation: BAA35943.1.
PIRiD40840.
RefSeqiNP_415641.1. NC_000913.3.
WP_000759317.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74207; AAC74207; b1123.
BAA35943; BAA35943; BAA35943.
GeneIDi945682.
KEGGiecj:JW1109.
eco:b1123.
PATRICi32117493. VBIEscCol129921_1169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37041.1.
U00096 Genomic DNA. Translation: AAC74207.1.
AP009048 Genomic DNA. Translation: BAA35943.1.
PIRiD40840.
RefSeqiNP_415641.1. NC_000913.3.
WP_000759317.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POTX-ray1.80A24-348[»]
1POYX-ray2.501/2/3/426-348[»]
ProteinModelPortaliP0AFK9.
SMRiP0AFK9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261797. 10 interactors.
IntActiP0AFK9. 7 interactors.
STRINGi511145.b1123.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AFK9.

Proteomic databases

EPDiP0AFK9.
PaxDbiP0AFK9.
PRIDEiP0AFK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74207; AAC74207; b1123.
BAA35943; BAA35943; BAA35943.
GeneIDi945682.
KEGGiecj:JW1109.
eco:b1123.
PATRICi32117493. VBIEscCol129921_1169.

Organism-specific databases

EchoBASEiEB0745.
EcoGeneiEG10752. potD.

Phylogenomic databases

eggNOGiENOG4107RUE. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263814.
InParanoidiP0AFK9.
KOiK11069.
OMAiMDDSREV.
PhylomeDBiP0AFK9.

Enzyme and pathway databases

BioCyciEcoCyc:POTD-MONOMER.
ECOL316407:JW1109-MONOMER.
MetaCyc:POTD-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFK9.
PROiP0AFK9.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.
ProtoNetiSearch...

Entry informationi

Entry nameiPOTD_ECOLI
AccessioniPrimary (citable) accession number: P0AFK9
Secondary accession number(s): P23861
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.