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P0AFK4 (POTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spermidine/putrescine transport system permease protein PotB
Gene names
Name:potB
Ordered Locus Names:b1125, JW1111
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.

Contains 1 ABC transmembrane type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Spermidine/putrescine transport system permease protein PotB
PRO_0000060174

Regions

Transmembrane1 – 2121Helical; Potential
Topological domain22 – 6039Periplasmic Potential
Transmembrane61 – 8121Helical; Potential
Topological domain82 – 898Cytoplasmic Potential
Transmembrane90 – 11021Helical; Potential
Topological domain111 – 13525Periplasmic Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 18731Cytoplasmic Potential
Transmembrane188 – 20821Helical; Potential
Topological domain209 – 24133Periplasmic Potential
Transmembrane242 – 26221Helical; Potential
Topological domain263 – 27513Cytoplasmic Potential
Domain55 – 261207ABC transmembrane type-1

Sequences

Sequence LengthMass (Da)Tools
P0AFK4 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 39D83EF14531D657

FASTA27531,062
        10         20         30         40         50         60 
MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL YFEVLLHSLN 

        70         80         90        100        110        120 
MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW TNSLIRIYGL KIFLSTKGYL 

       130        140        150        160        170        180 
NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI LLPFMVMPLY SSIEKLDKPL LEAARDLGAS 

       190        200        210        220        230        240 
KLQTFIRIII PLTMPGIIAG CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW 

       250        260        270 
PFGAATSITL TIVMGLMLLV YWRASRLLNK KVELE 

« Hide

References

« Hide 'large scale' references
[1]"Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRB40840.
RefSeqNP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AFK4.
SMRP0AFK4. Positions 31-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b1125.

Protein family/group databases

TCDB3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbP0AFK4.
PRIDEP0AFK4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneID12932679.
945692.
KEGGecj:Y75_p1095.
eco:b1125.
PATRIC32117497. VBIEscCol129921_1171.

Organism-specific databases

EchoBASEEB0743.
EcoGeneEG10750. potB.

Phylogenomic databases

eggNOGCOG1176.
HOGENOMHOG000263581.
KOK11071.
OMANSFWYAF.
OrthoDBEOG6HMXBZ.
PhylomeDBP0AFK4.
ProtClustDBPRK09497.

Enzyme and pathway databases

BioCycEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.

Gene expression databases

GenevestigatorP0AFK4.

Family and domain databases

Gene3D1.10.3720.10. 1 hit.
InterProIPR000515. MetI-like.
[Graphical view]
PfamPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMSSF161098. SSF161098. 1 hit.
PROSITEPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AFK4.

Entry information

Entry namePOTB_ECOLI
AccessionPrimary (citable) accession number: P0AFK4
Secondary accession number(s): P23860
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene