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P0AFK4

- POTB_ECOLI

UniProt

P0AFK4 - POTB_ECOLI

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Protein

Spermidine/putrescine transport system permease protein PotB

Gene

potB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

GO - Molecular functioni

  1. polyamine-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  1. polyamine transmembrane transport Source: GOC
  2. polyamine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine transport system permease protein PotB
Gene namesi
Name:potB
Ordered Locus Names:b1125, JW1111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10750. potB.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: EcoCyc
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Spermidine/putrescine transport system permease protein PotBPRO_0000060174Add
BLAST

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Expressioni

Gene expression databases

GenevestigatoriP0AFK4.

Interactioni

Protein-protein interaction databases

STRINGi511145.b1125.

Structurei

3D structure databases

ProteinModelPortaliP0AFK4.
SMRiP0AFK4. Positions 31-203.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 6039PeriplasmicSequence AnalysisAdd
BLAST
Topological domaini82 – 898CytoplasmicSequence Analysis
Topological domaini111 – 13525PeriplasmicSequence AnalysisAdd
BLAST
Topological domaini157 – 18731CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini209 – 24133PeriplasmicSequence AnalysisAdd
BLAST
Topological domaini263 – 27513CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei61 – 8121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei90 – 11021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei136 – 15621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei188 – 20821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei242 – 26221HelicalPROSITE-ProRule annotationAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 261207ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1176.
HOGENOMiHOG000263581.
InParanoidiP0AFK4.
KOiK11071.
OMAiPLRIMYT.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP0AFK4.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFK4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL
60 70 80 90 100
YFEVLLHSLN MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW
110 120 130 140 150
TNSLIRIYGL KIFLSTKGYL NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI
160 170 180 190 200
LLPFMVMPLY SSIEKLDKPL LEAARDLGAS KLQTFIRIII PLTMPGIIAG
210 220 230 240 250
CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW PFGAATSITL
260 270
TIVMGLMLLV YWRASRLLNK KVELE
Length:275
Mass (Da):31,062
Last modified:December 20, 2005 - v1
Checksum:i39D83EF14531D657
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi12932679.
945692.
KEGGiecj:Y75_p1095.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1 .
U00096 Genomic DNA. Translation: AAC74209.1 .
AP009048 Genomic DNA. Translation: BAA35945.1 .
PIRi B40840.
RefSeqi NP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AFK4.
SMRi P0AFK4. Positions 31-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1125.

Protein family/group databases

TCDBi 3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbi P0AFK4.
PRIDEi P0AFK4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74209 ; AAC74209 ; b1125 .
BAA35945 ; BAA35945 ; BAA35945 .
GeneIDi 12932679.
945692.
KEGGi ecj:Y75_p1095.
eco:b1125.
PATRICi 32117497. VBIEscCol129921_1171.

Organism-specific databases

EchoBASEi EB0743.
EcoGenei EG10750. potB.

Phylogenomic databases

eggNOGi COG1176.
HOGENOMi HOG000263581.
InParanoidi P0AFK4.
KOi K11071.
OMAi PLRIMYT.
OrthoDBi EOG6HMXBZ.
PhylomeDBi P0AFK4.

Enzyme and pathway databases

BioCyci EcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Miscellaneous databases

PROi P0AFK4.

Gene expression databases

Genevestigatori P0AFK4.

Family and domain databases

Gene3Di 1.10.3720.10. 1 hit.
InterProi IPR000515. MetI-like.
[Graphical view ]
Pfami PF00528. BPD_transp_1. 1 hit.
[Graphical view ]
SUPFAMi SSF161098. SSF161098. 1 hit.
PROSITEi PS50928. ABC_TM1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
    Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
    J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AFK4
Secondary accession number(s): P23860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3