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P0AFK4

- POTB_ECOLI

UniProt

P0AFK4 - POTB_ECOLI

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Protein
Spermidine/putrescine transport system permease protein PotB
Gene
potB, b1125, JW1111
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

GO - Molecular functioni

  1. polyamine-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  1. polyamine transmembrane transport Source: GOC
  2. polyamine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine transport system permease protein PotB
Gene namesi
Name:potB
Ordered Locus Names:b1125, JW1111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10750. potB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed prediction
Add
BLAST
Topological domaini22 – 6039Periplasmic Reviewed prediction
Add
BLAST
Transmembranei61 – 8121Helical; Reviewed prediction
Add
BLAST
Topological domaini82 – 898Cytoplasmic Reviewed prediction
Transmembranei90 – 11021Helical; Reviewed prediction
Add
BLAST
Topological domaini111 – 13525Periplasmic Reviewed prediction
Add
BLAST
Transmembranei136 – 15621Helical; Reviewed prediction
Add
BLAST
Topological domaini157 – 18731Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei188 – 20821Helical; Reviewed prediction
Add
BLAST
Topological domaini209 – 24133Periplasmic Reviewed prediction
Add
BLAST
Transmembranei242 – 26221Helical; Reviewed prediction
Add
BLAST
Topological domaini263 – 27513Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: EcoCyc
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Spermidine/putrescine transport system permease protein PotB
PRO_0000060174Add
BLAST

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Expressioni

Gene expression databases

GenevestigatoriP0AFK4.

Interactioni

Protein-protein interaction databases

STRINGi511145.b1125.

Structurei

3D structure databases

ProteinModelPortaliP0AFK4.
SMRiP0AFK4. Positions 31-203.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 261207ABC transmembrane type-1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1176.
HOGENOMiHOG000263581.
KOiK11071.
OMAiPLRIMYT.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP0AFK4.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFK4-1 [UniParc]FASTAAdd to Basket

« Hide

MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL    50
YFEVLLHSLN MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW 100
TNSLIRIYGL KIFLSTKGYL NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI 150
LLPFMVMPLY SSIEKLDKPL LEAARDLGAS KLQTFIRIII PLTMPGIIAG 200
CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW PFGAATSITL 250
TIVMGLMLLV YWRASRLLNK KVELE 275
Length:275
Mass (Da):31,062
Last modified:December 20, 2005 - v1
Checksum:i39D83EF14531D657
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi12932679.
945692.
KEGGiecj:Y75_p1095.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1 .
U00096 Genomic DNA. Translation: AAC74209.1 .
AP009048 Genomic DNA. Translation: BAA35945.1 .
PIRi B40840.
RefSeqi NP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AFK4.
SMRi P0AFK4. Positions 31-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1125.

Protein family/group databases

TCDBi 3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbi P0AFK4.
PRIDEi P0AFK4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74209 ; AAC74209 ; b1125 .
BAA35945 ; BAA35945 ; BAA35945 .
GeneIDi 12932679.
945692.
KEGGi ecj:Y75_p1095.
eco:b1125.
PATRICi 32117497. VBIEscCol129921_1171.

Organism-specific databases

EchoBASEi EB0743.
EcoGenei EG10750. potB.

Phylogenomic databases

eggNOGi COG1176.
HOGENOMi HOG000263581.
KOi K11071.
OMAi PLRIMYT.
OrthoDBi EOG6HMXBZ.
PhylomeDBi P0AFK4.

Enzyme and pathway databases

BioCyci EcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Miscellaneous databases

PROi P0AFK4.

Gene expression databases

Genevestigatori P0AFK4.

Family and domain databases

Gene3Di 1.10.3720.10. 1 hit.
InterProi IPR000515. MetI-like.
[Graphical view ]
Pfami PF00528. BPD_transp_1. 1 hit.
[Graphical view ]
SUPFAMi SSF161098. SSF161098. 1 hit.
PROSITEi PS50928. ABC_TM1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
    Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
    J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AFK4
Secondary accession number(s): P23860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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