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P0AFK4

- POTB_ECOLI

UniProt

P0AFK4 - POTB_ECOLI

Protein

Spermidine/putrescine transport system permease protein PotB

Gene

potB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

    GO - Molecular functioni

    1. polyamine-transporting ATPase activity Source: EcoCyc

    GO - Biological processi

    1. polyamine transmembrane transport Source: GOC
    2. polyamine transport Source: EcoCyc

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:POTB-MONOMER.
    ECOL316407:JW1111-MONOMER.
    MetaCyc:POTB-MONOMER.
    RETL1328306-WGS:GSTH-5713-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spermidine/putrescine transport system permease protein PotB
    Gene namesi
    Name:potB
    Ordered Locus Names:b1125, JW1111
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10750. potB.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: EcoCyc
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Spermidine/putrescine transport system permease protein PotBPRO_0000060174Add
    BLAST

    Proteomic databases

    PaxDbiP0AFK4.
    PRIDEiP0AFK4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AFK4.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b1125.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AFK4.
    SMRiP0AFK4. Positions 31-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 6039PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 898CytoplasmicSequence Analysis
    Topological domaini111 – 13525PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini157 – 18731CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini209 – 24133PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini263 – 27513CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei61 – 8121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei90 – 11021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei136 – 15621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei188 – 20821HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei242 – 26221HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 261207ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1176.
    HOGENOMiHOG000263581.
    KOiK11071.
    OMAiPLRIMYT.
    OrthoDBiEOG6HMXBZ.
    PhylomeDBiP0AFK4.

    Family and domain databases

    Gene3Di1.10.3720.10. 1 hit.
    InterProiIPR000515. MetI-like.
    [Graphical view]
    PfamiPF00528. BPD_transp_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF161098. SSF161098. 1 hit.
    PROSITEiPS50928. ABC_TM1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AFK4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL    50
    YFEVLLHSLN MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW 100
    TNSLIRIYGL KIFLSTKGYL NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI 150
    LLPFMVMPLY SSIEKLDKPL LEAARDLGAS KLQTFIRIII PLTMPGIIAG 200
    CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW PFGAATSITL 250
    TIVMGLMLLV YWRASRLLNK KVELE 275
    Length:275
    Mass (Da):31,062
    Last modified:December 20, 2005 - v1
    Checksum:i39D83EF14531D657
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64519 Genomic DNA. Translation: AAC37039.1.
    U00096 Genomic DNA. Translation: AAC74209.1.
    AP009048 Genomic DNA. Translation: BAA35945.1.
    PIRiB40840.
    RefSeqiNP_415643.3. NC_000913.3.
    YP_489393.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74209; AAC74209; b1125.
    BAA35945; BAA35945; BAA35945.
    GeneIDi12932679.
    945692.
    KEGGiecj:Y75_p1095.
    eco:b1125.
    PATRICi32117497. VBIEscCol129921_1171.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64519 Genomic DNA. Translation: AAC37039.1 .
    U00096 Genomic DNA. Translation: AAC74209.1 .
    AP009048 Genomic DNA. Translation: BAA35945.1 .
    PIRi B40840.
    RefSeqi NP_415643.3. NC_000913.3.
    YP_489393.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AFK4.
    SMRi P0AFK4. Positions 31-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b1125.

    Protein family/group databases

    TCDBi 3.A.1.11.1. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    PaxDbi P0AFK4.
    PRIDEi P0AFK4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74209 ; AAC74209 ; b1125 .
    BAA35945 ; BAA35945 ; BAA35945 .
    GeneIDi 12932679.
    945692.
    KEGGi ecj:Y75_p1095.
    eco:b1125.
    PATRICi 32117497. VBIEscCol129921_1171.

    Organism-specific databases

    EchoBASEi EB0743.
    EcoGenei EG10750. potB.

    Phylogenomic databases

    eggNOGi COG1176.
    HOGENOMi HOG000263581.
    KOi K11071.
    OMAi PLRIMYT.
    OrthoDBi EOG6HMXBZ.
    PhylomeDBi P0AFK4.

    Enzyme and pathway databases

    BioCyci EcoCyc:POTB-MONOMER.
    ECOL316407:JW1111-MONOMER.
    MetaCyc:POTB-MONOMER.
    RETL1328306-WGS:GSTH-5713-MONOMER.

    Miscellaneous databases

    PROi P0AFK4.

    Gene expression databases

    Genevestigatori P0AFK4.

    Family and domain databases

    Gene3Di 1.10.3720.10. 1 hit.
    InterProi IPR000515. MetI-like.
    [Graphical view ]
    Pfami PF00528. BPD_transp_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF161098. SSF161098. 1 hit.
    PROSITEi PS50928. ABC_TM1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
      Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
      J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiPOTB_ECOLI
    AccessioniPrimary (citable) accession number: P0AFK4
    Secondary accession number(s): P23860
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3