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Protein

Spermidine/putrescine transport system permease protein PotB

Gene

potB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

GO - Molecular functioni

  • polyamine-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • polyamine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine transport system permease protein PotB
Gene namesi
Name:potB
Ordered Locus Names:b1125, JW1111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10750. potB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini22 – 60PeriplasmicSequence analysisAdd BLAST39
Transmembranei61 – 81HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini82 – 89CytoplasmicSequence analysis8
Transmembranei90 – 110HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini111 – 135PeriplasmicSequence analysisAdd BLAST25
Transmembranei136 – 156HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini157 – 187CytoplasmicSequence analysisAdd BLAST31
Transmembranei188 – 208HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini209 – 241PeriplasmicSequence analysisAdd BLAST33
Transmembranei242 – 262HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini263 – 275CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000601741 – 275Spermidine/putrescine transport system permease protein PotBAdd BLAST275

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Interactioni

Protein-protein interaction databases

BioGridi4261673. 9 interactors.
STRINGi511145.b1125.

Structurei

3D structure databases

ProteinModelPortaliP0AFK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 261ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST207

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F38. Bacteria.
COG1176. LUCA.
HOGENOMiHOG000263581.
InParanoidiP0AFK4.
KOiK11071.
OMAiQDWGMGA.
PhylomeDBiP0AFK4.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL
60 70 80 90 100
YFEVLLHSLN MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW
110 120 130 140 150
TNSLIRIYGL KIFLSTKGYL NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI
160 170 180 190 200
LLPFMVMPLY SSIEKLDKPL LEAARDLGAS KLQTFIRIII PLTMPGIIAG
210 220 230 240 250
CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW PFGAATSITL
260 270
TIVMGLMLLV YWRASRLLNK KVELE
Length:275
Mass (Da):31,062
Last modified:December 20, 2005 - v1
Checksum:i39D83EF14531D657
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi945692.
KEGGiecj:JW1111.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AFK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261673. 9 interactors.
STRINGi511145.b1125.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi945692.
KEGGiecj:JW1111.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Organism-specific databases

EchoBASEiEB0743.
EcoGeneiEG10750. potB.

Phylogenomic databases

eggNOGiENOG4105F38. Bacteria.
COG1176. LUCA.
HOGENOMiHOG000263581.
InParanoidiP0AFK4.
KOiK11071.
OMAiQDWGMGA.
PhylomeDBiP0AFK4.

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.

Miscellaneous databases

PROiP0AFK4.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AFK4
Secondary accession number(s): P23860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.