P0AFK4 (POTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine transport system permease protein PotB

Gene names

Name:	potB
Ordered Locus Names:	b1125, JW1111

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from direct assay Ref.1. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	polyamine-transporting ATPase activity Inferred from mutant phenotype PubMed 8366082. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Spermidine/putrescine transport system permease protein PotB		PRO_0000060174
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Topological domain	22 – 60	39	Periplasmic Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Topological domain	82 – 89	8	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 135	25	Periplasmic Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Topological domain	157 – 187	31	Cytoplasmic Potential
	Transmembrane	188 – 208	21	Helical; Potential
	Topological domain	209 – 241	33	Periplasmic Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Topological domain	263 – 275	13	Cytoplasmic Potential
	Domain	55 – 261	207	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AFK4 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 39D83EF14531D657	FASTA	275	31,062
	10 20 30 40 50 60 MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL YFEVLLHSLN 70 80 90 100 110 120 MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW TNSLIRIYGL KIFLSTKGYL 130 140 150 160 170 180 NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI LLPFMVMPLY SSIEKLDKPL LEAARDLGAS 190 200 210 220 230 240 KLQTFIRIII PLTMPGIIAG CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW 250 260 270 PFGAATSITL TIVMGLMLLV YWRASRLLNK KVELE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome." Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K. J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64519 Genomic DNA. Translation: AAC37039.1. U00096 Genomic DNA. Translation: AAC74209.1. AP009048 Genomic DNA. Translation: BAA35945.1.
PIR	B40840.
RefSeq	NP_415643.3. NC_000913.2. YP_489393.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFK4.
SMR	P0AFK4. Positions 31-203.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1125.
Protein family/group databases
TCDB	3.A.1.11.1. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PaxDb	P0AFK4.
PRIDE	P0AFK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74209; AAC74209; b1125. BAA35945; BAA35945; BAA35945.
GeneID	12932679. 945692.
KEGG	ecj:Y75_p1095. eco:b1125.
PATRIC	32117497. VBIEscCol129921_1171.
Organism-specific databases
EchoBASE	EB0743.
EcoGene	EG10750. potB.
Phylogenomic databases
eggNOG	COG1176.
HOGENOM	HOG000263581.
KO	K11071.
OMA	EVAVIIG.
ProtClustDB	PRK09497.
Enzyme and pathway databases
BioCyc	EcoCyc:POTB-MONOMER. ECOL316407:JW1111-MONOMER.
Gene expression databases
Genevestigator	P0AFK4.
Family and domain databases
InterPro	IPR000515. BPD_transp. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTB_ECOLI

Accession

Primary (citable) accession number: P0AFK4
Secondary accession number(s): P23860

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program