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Protein

Spermidine/putrescine transport system permease protein PotB

Gene

potB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine.

GO - Molecular functioni

  1. polyamine-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  1. polyamine transmembrane transport Source: GOC
  2. polyamine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine transport system permease protein PotB
Gene namesi
Name:potB
Ordered Locus Names:b1125, JW1111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10750. potB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini22 – 6039PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini82 – 898CytoplasmicSequence Analysis
Transmembranei90 – 11021HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini111 – 13525PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei136 – 15621HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini157 – 18731CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini209 – 24133PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei242 – 26221HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini263 – 27513CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: EcoCyc
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Spermidine/putrescine transport system permease protein PotBPRO_0000060174Add
BLAST

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Expressioni

Gene expression databases

GenevestigatoriP0AFK4.

Interactioni

Protein-protein interaction databases

STRINGi511145.b1125.

Structurei

3D structure databases

ProteinModelPortaliP0AFK4.
SMRiP0AFK4. Positions 31-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 261207ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1176.
HOGENOMiHOG000263581.
InParanoidiP0AFK4.
KOiK11071.
OMAiLIIPTWI.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP0AFK4.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL
60 70 80 90 100
YFEVLLHSLN MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW
110 120 130 140 150
TNSLIRIYGL KIFLSTKGYL NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI
160 170 180 190 200
LLPFMVMPLY SSIEKLDKPL LEAARDLGAS KLQTFIRIII PLTMPGIIAG
210 220 230 240 250
CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW PFGAATSITL
260 270
TIVMGLMLLV YWRASRLLNK KVELE
Length:275
Mass (Da):31,062
Last modified:December 20, 2005 - v1
Checksum:i39D83EF14531D657
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi12932679.
945692.
KEGGiecj:Y75_p1095.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64519 Genomic DNA. Translation: AAC37039.1.
U00096 Genomic DNA. Translation: AAC74209.1.
AP009048 Genomic DNA. Translation: BAA35945.1.
PIRiB40840.
RefSeqiNP_415643.3. NC_000913.3.
YP_489393.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0AFK4.
SMRiP0AFK4. Positions 31-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b1125.

Protein family/group databases

TCDBi3.A.1.11.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFK4.
PRIDEiP0AFK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74209; AAC74209; b1125.
BAA35945; BAA35945; BAA35945.
GeneIDi12932679.
945692.
KEGGiecj:Y75_p1095.
eco:b1125.
PATRICi32117497. VBIEscCol129921_1171.

Organism-specific databases

EchoBASEiEB0743.
EcoGeneiEG10750. potB.

Phylogenomic databases

eggNOGiCOG1176.
HOGENOMiHOG000263581.
InParanoidiP0AFK4.
KOiK11071.
OMAiLIIPTWI.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP0AFK4.

Enzyme and pathway databases

BioCyciEcoCyc:POTB-MONOMER.
ECOL316407:JW1111-MONOMER.
MetaCyc:POTB-MONOMER.
RETL1328306-WGS:GSTH-5713-MONOMER.

Miscellaneous databases

PROiP0AFK4.

Gene expression databases

GenevestigatoriP0AFK4.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
    Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
    J. Biol. Chem. 266:20928-20933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AFK4
Secondary accession number(s): P23860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.