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Protein

Nicotinamide riboside transporter PnuC

Gene

pnuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for nicotinamide riboside transport across the inner membrane.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188Nicotinamide ribosideBy similarity1
Binding sitei192Nicotinamide ribosideBy similarity1

GO - Molecular functioni

  • nicotinamide riboside transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • nicotinamide riboside transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:PNUC-MONOMER.
ECOL316407:JW0734-MONOMER.
MetaCyc:PNUC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide riboside transporter PnuC
Gene namesi
Name:pnuC
Ordered Locus Names:b0751, JW0734
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11700. pnuC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 48PeriplasmicSequence analysis6
Transmembranei49 – 68HelicalSequence analysisAdd BLAST20
Topological domaini69 – 71CytoplasmicSequence analysis3
Transmembranei72 – 89HelicalSequence analysisAdd BLAST18
Topological domaini90 – 109PeriplasmicSequence analysisAdd BLAST20
Transmembranei110 – 127HelicalSequence analysisAdd BLAST18
Topological domaini128 – 157CytoplasmicSequence analysisAdd BLAST30
Transmembranei158 – 177HelicalSequence analysisAdd BLAST20
Topological domaini178 – 183PeriplasmicSequence analysis6
Transmembranei184 – 206HelicalSequence analysisAdd BLAST23
Topological domaini207 – 239Cytoplasmic1 PublicationAdd BLAST33

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584881 – 239Nicotinamide riboside transporter PnuCAdd BLAST239

Proteomic databases

PaxDbiP0AFK2.
PRIDEiP0AFK2.

Expressioni

Inductioni

Repressed by NadR.

Interactioni

Protein-protein interaction databases

BioGridi4259938. 2 interactors.
STRINGi511145.b0751.

Structurei

3D structure databases

ProteinModelPortaliP0AFK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106Z1T. Bacteria.
COG3201. LUCA.
HOGENOMiHOG000144393.
InParanoidiP0AFK2.
KOiK03811.
OMAiGGYAMSW.
PhylomeDBiP0AFK2.

Family and domain databases

InterProiIPR006419. NMN_transpt_PnuC.
[Graphical view]
PfamiPF04973. NMN_transporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01528. NMN_trans_PnuC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFFSVQNIL VHIPIGAGGY DLSWIEAVGT IAGLLCIGLA SLEKISNYFF
60 70 80 90 100
GLINVTLFGI IFFQIQLYAS LLLQVFFFAA NIYGWYAWSR QTSQNEAELK
110 120 130 140 150
IRWLPLPKAL SWLAVCVVSI GLMTVFINPV FAFLTRVAVM IMQALGLQVV
160 170 180 190 200
MPELQPDAFP FWDSCMMVLS IVAMILMTRK YVENWLLWVI INVISVVIFA
210 220 230
LQGVYAMSLE YIILTFIALN GSRMWINSAR ERGSRALSH
Length:239
Mass (Da):26,996
Last modified:December 20, 2005 - v1
Checksum:i5A01FC261A16CF61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7VQ → TH (PubMed:2841129).Curated2
Sequence conflicti11V → I (PubMed:2841129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73838.1.
AP009048 Genomic DNA. Translation: BAA35413.1.
X12713 Genomic DNA. No translation available.
PIRiG64810.
RefSeqiNP_415272.1. NC_000913.3.
WP_000345410.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73838; AAC73838; b0751.
BAA35413; BAA35413; BAA35413.
GeneIDi945350.
KEGGiecj:JW0734.
eco:b0751.
PATRICi32116701. VBIEscCol129921_0776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73838.1.
AP009048 Genomic DNA. Translation: BAA35413.1.
X12713 Genomic DNA. No translation available.
PIRiG64810.
RefSeqiNP_415272.1. NC_000913.3.
WP_000345410.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259938. 2 interactors.
STRINGi511145.b0751.

Proteomic databases

PaxDbiP0AFK2.
PRIDEiP0AFK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73838; AAC73838; b0751.
BAA35413; BAA35413; BAA35413.
GeneIDi945350.
KEGGiecj:JW0734.
eco:b0751.
PATRICi32116701. VBIEscCol129921_0776.

Organism-specific databases

EchoBASEiEB1651.
EcoGeneiEG11700. pnuC.

Phylogenomic databases

eggNOGiENOG4106Z1T. Bacteria.
COG3201. LUCA.
HOGENOMiHOG000144393.
InParanoidiP0AFK2.
KOiK03811.
OMAiGGYAMSW.
PhylomeDBiP0AFK2.

Enzyme and pathway databases

BioCyciEcoCyc:PNUC-MONOMER.
ECOL316407:JW0734-MONOMER.
MetaCyc:PNUC-MONOMER.

Miscellaneous databases

PROiP0AFK2.

Family and domain databases

InterProiIPR006419. NMN_transpt_PnuC.
[Graphical view]
PfamiPF04973. NMN_transporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01528. NMN_trans_PnuC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNUC_ECOLI
AccessioniPrimary (citable) accession number: P0AFK2
Secondary accession number(s): P31215, P77227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.