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Protein

Nicotinamide riboside transporter PnuC

Gene

pnuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for nicotinamide riboside transport across the inner membrane.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei188 – 1881Nicotinamide ribosideBy similarity
Binding sitei192 – 1921Nicotinamide ribosideBy similarity

GO - Molecular functioni

  • nicotinamide riboside transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • nicotinamide riboside transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:PNUC-MONOMER.
ECOL316407:JW0734-MONOMER.
MetaCyc:PNUC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide riboside transporter PnuC
Gene namesi
Name:pnuC
Ordered Locus Names:b0751, JW0734
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11700. pnuC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Topological domaini43 – 486PeriplasmicSequence analysis
Transmembranei49 – 6820HelicalSequence analysisAdd
BLAST
Topological domaini69 – 713CytoplasmicSequence analysis
Transmembranei72 – 8918HelicalSequence analysisAdd
BLAST
Topological domaini90 – 10920PeriplasmicSequence analysisAdd
BLAST
Transmembranei110 – 12718HelicalSequence analysisAdd
BLAST
Topological domaini128 – 15730CytoplasmicSequence analysisAdd
BLAST
Transmembranei158 – 17720HelicalSequence analysisAdd
BLAST
Topological domaini178 – 1836PeriplasmicSequence analysis
Transmembranei184 – 20623HelicalSequence analysisAdd
BLAST
Topological domaini207 – 23933Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Nicotinamide riboside transporter PnuCPRO_0000058488Add
BLAST

Proteomic databases

PaxDbiP0AFK2.

Expressioni

Inductioni

Repressed by NadR.

Interactioni

Protein-protein interaction databases

BioGridi4259938. 2 interactions.
STRINGi511145.b0751.

Structurei

3D structure databases

ProteinModelPortaliP0AFK2.
SMRiP0AFK2. Positions 23-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106Z1T. Bacteria.
COG3201. LUCA.
HOGENOMiHOG000144393.
InParanoidiP0AFK2.
KOiK03811.
OMAiGGYAMSW.
PhylomeDBiP0AFK2.

Family and domain databases

InterProiIPR006419. NMN_transpt_PnuC.
[Graphical view]
PfamiPF04973. NMN_transporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01528. NMN_trans_PnuC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFFSVQNIL VHIPIGAGGY DLSWIEAVGT IAGLLCIGLA SLEKISNYFF
60 70 80 90 100
GLINVTLFGI IFFQIQLYAS LLLQVFFFAA NIYGWYAWSR QTSQNEAELK
110 120 130 140 150
IRWLPLPKAL SWLAVCVVSI GLMTVFINPV FAFLTRVAVM IMQALGLQVV
160 170 180 190 200
MPELQPDAFP FWDSCMMVLS IVAMILMTRK YVENWLLWVI INVISVVIFA
210 220 230
LQGVYAMSLE YIILTFIALN GSRMWINSAR ERGSRALSH
Length:239
Mass (Da):26,996
Last modified:December 20, 2005 - v1
Checksum:i5A01FC261A16CF61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 72VQ → TH (PubMed:2841129).Curated
Sequence conflicti11 – 111V → I (PubMed:2841129).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73838.1.
AP009048 Genomic DNA. Translation: BAA35413.1.
X12713 Genomic DNA. No translation available.
PIRiG64810.
RefSeqiNP_415272.1. NC_000913.3.
WP_000345410.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73838; AAC73838; b0751.
BAA35413; BAA35413; BAA35413.
GeneIDi945350.
KEGGiecj:JW0734.
eco:b0751.
PATRICi32116701. VBIEscCol129921_0776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73838.1.
AP009048 Genomic DNA. Translation: BAA35413.1.
X12713 Genomic DNA. No translation available.
PIRiG64810.
RefSeqiNP_415272.1. NC_000913.3.
WP_000345410.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFK2.
SMRiP0AFK2. Positions 23-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259938. 2 interactions.
STRINGi511145.b0751.

Proteomic databases

PaxDbiP0AFK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73838; AAC73838; b0751.
BAA35413; BAA35413; BAA35413.
GeneIDi945350.
KEGGiecj:JW0734.
eco:b0751.
PATRICi32116701. VBIEscCol129921_0776.

Organism-specific databases

EchoBASEiEB1651.
EcoGeneiEG11700. pnuC.

Phylogenomic databases

eggNOGiENOG4106Z1T. Bacteria.
COG3201. LUCA.
HOGENOMiHOG000144393.
InParanoidiP0AFK2.
KOiK03811.
OMAiGGYAMSW.
PhylomeDBiP0AFK2.

Enzyme and pathway databases

BioCyciEcoCyc:PNUC-MONOMER.
ECOL316407:JW0734-MONOMER.
MetaCyc:PNUC-MONOMER.

Miscellaneous databases

PROiP0AFK2.

Family and domain databases

InterProiIPR006419. NMN_transpt_PnuC.
[Graphical view]
PfamiPF04973. NMN_transporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01528. NMN_trans_PnuC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNUC_ECOLI
AccessioniPrimary (citable) accession number: P0AFK2
Secondary accession number(s): P31215, P77227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.