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Protein

Phosphate regulon transcriptional regulatory protein PhoB

Gene

phoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi129 – 227OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST99

GO - Molecular functioni

  • bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • phosphorelay response regulator activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Phosphate transport, Transcription, Transcription regulation, Transport, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PHOB-MONOMER.
ECOL316407:JW0389-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate regulon transcriptional regulatory protein PhoB
Gene namesi
Name:phoB
Ordered Locus Names:b0399, JW0389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10728. phoB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811861 – 229Phosphate regulon transcriptional regulatory protein PhoBAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei534-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Phosphorylated by PhoR or CreC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFJ5.
PRIDEiP0AFJ5.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1116564,EBI-1116564

GO - Molecular functioni

  • bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259816. 15 interactors.
DIPiDIP-35852N.
IntActiP0AFJ5. 24 interactors.
MINTiMINT-1313174.
STRINGi511145.b0399.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi12 – 24Combined sources13
Beta strandi28 – 32Combined sources5
Helixi35 – 39Combined sources5
Beta strandi43 – 45Combined sources3
Beta strandi48 – 56Combined sources9
Helixi61 – 69Combined sources9
Turni72 – 76Combined sources5
Beta strandi79 – 84Combined sources6
Helixi87 – 92Combined sources6
Beta strandi93 – 95Combined sources3
Beta strandi100 – 106Combined sources7
Helixi109 – 121Combined sources13
Beta strandi132 – 134Combined sources3
Beta strandi137 – 140Combined sources4
Turni141 – 144Combined sources4
Beta strandi145 – 148Combined sources4
Beta strandi151 – 153Combined sources3
Helixi157 – 168Combined sources12
Beta strandi171 – 174Combined sources4
Helixi176 – 183Combined sources8
Beta strandi186 – 188Combined sources3
Helixi193 – 206Combined sources14
Helixi207 – 209Combined sources3
Helixi211 – 214Combined sources4
Beta strandi215 – 218Combined sources4
Turni219 – 221Combined sources3
Beta strandi222 – 225Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B00X-ray1.88A/B1-127[»]
1GXPX-ray2.50A/B/E/F124-229[»]
1GXQX-ray2.00A124-229[»]
1QQINMR-A126-229[»]
1ZESX-ray1.90A/B/C1-125[»]
2IYNX-ray2.08A/B/C1-127[»]
2JB9X-ray1.70A/B1-127[»]
2JBAX-ray1.45A/B1-127[»]
2Z33NMR-A126-229[»]
3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]
ProteinModelPortaliP0AFJ5.
SMRiP0AFJ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFJ5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 120Response regulatoryPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CK6. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP0AFJ5.
KOiK07657.
OMAiMAMRYEG.
PhylomeDBiP0AFJ5.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR011879. Sig_transdc_resp-reg_PhoB.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02154. PhoB. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI
60 70 80 90 100
LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD
110 120 130 140 150
DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE
160 170 180 190 200
EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR
210 220
RLRKALEPGG HDRMVQTVRG TGYRFSTRF
Length:229
Mass (Da):26,433
Last modified:August 1, 1988 - v1
Checksum:iEB46BA282F1F2C23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04026 Genomic DNA. Translation: CAA27659.1.
U73857 Genomic DNA. Translation: AAB18123.1.
U00096 Genomic DNA. Translation: AAC73502.1.
AP009048 Genomic DNA. Translation: BAE76179.1.
PIRiA24256. RGECFB.
RefSeqiNP_414933.1. NC_000913.3.
WP_000113933.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73502; AAC73502; b0399.
BAE76179; BAE76179; BAE76179.
GeneIDi945046.
KEGGiecj:JW0389.
eco:b0399.
PATRICi32115943. VBIEscCol129921_0413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04026 Genomic DNA. Translation: CAA27659.1.
U73857 Genomic DNA. Translation: AAB18123.1.
U00096 Genomic DNA. Translation: AAC73502.1.
AP009048 Genomic DNA. Translation: BAE76179.1.
PIRiA24256. RGECFB.
RefSeqiNP_414933.1. NC_000913.3.
WP_000113933.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B00X-ray1.88A/B1-127[»]
1GXPX-ray2.50A/B/E/F124-229[»]
1GXQX-ray2.00A124-229[»]
1QQINMR-A126-229[»]
1ZESX-ray1.90A/B/C1-125[»]
2IYNX-ray2.08A/B/C1-127[»]
2JB9X-ray1.70A/B1-127[»]
2JBAX-ray1.45A/B1-127[»]
2Z33NMR-A126-229[»]
3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]
ProteinModelPortaliP0AFJ5.
SMRiP0AFJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259816. 15 interactors.
DIPiDIP-35852N.
IntActiP0AFJ5. 24 interactors.
MINTiMINT-1313174.
STRINGi511145.b0399.

Proteomic databases

PaxDbiP0AFJ5.
PRIDEiP0AFJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73502; AAC73502; b0399.
BAE76179; BAE76179; BAE76179.
GeneIDi945046.
KEGGiecj:JW0389.
eco:b0399.
PATRICi32115943. VBIEscCol129921_0413.

Organism-specific databases

EchoBASEiEB0721.
EcoGeneiEG10728. phoB.

Phylogenomic databases

eggNOGiENOG4105CK6. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP0AFJ5.
KOiK07657.
OMAiMAMRYEG.
PhylomeDBiP0AFJ5.

Enzyme and pathway databases

BioCyciEcoCyc:PHOB-MONOMER.
ECOL316407:JW0389-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFJ5.
PROiP0AFJ5.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR011879. Sig_transdc_resp-reg_PhoB.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02154. PhoB. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHOB_ECOLI
AccessioniPrimary (citable) accession number: P0AFJ5
Secondary accession number(s): P08402, Q2MC27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.