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P0AFJ5

- PHOB_ECOLI

UniProt

P0AFJ5 - PHOB_ECOLI

Protein

Phosphate regulon transcriptional regulatory protein PhoB

Gene

phoB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

    GO - Molecular functioni

    1. bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
    2. DNA binding Source: UniProtKB-KW
    3. identical protein binding Source: IntAct
    4. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. phosphate ion transport Source: UniProtKB-KW
    2. regulation of DNA-templated transcription, initiation Source: EcoCyc
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Phosphate transport, Transcription, Transcription regulation, Transport, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOB-MONOMER.
    ECOL316407:JW0389-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphate regulon transcriptional regulatory protein PhoB
    Gene namesi
    Name:phoB
    Ordered Locus Names:b0399, JW0389
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10728. phoB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Phosphate regulon transcriptional regulatory protein PhoBPRO_0000081186Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 5314-aspartylphosphate1 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by PhoR or CreC.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP0AFJ5.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-1116564,EBI-1116564

    Protein-protein interaction databases

    DIPiDIP-35852N.
    IntActiP0AFJ5. 24 interactions.
    MINTiMINT-1313174.
    STRINGi511145.b0399.

    Structurei

    Secondary structure

    1
    229
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Helixi12 – 2413
    Beta strandi28 – 325
    Helixi35 – 395
    Beta strandi43 – 453
    Beta strandi48 – 569
    Helixi61 – 699
    Turni72 – 765
    Beta strandi79 – 846
    Helixi87 – 926
    Beta strandi100 – 1067
    Helixi109 – 12113
    Beta strandi132 – 1343
    Beta strandi137 – 1404
    Turni141 – 1444
    Beta strandi145 – 1484
    Beta strandi151 – 1533
    Helixi157 – 16812
    Beta strandi171 – 1744
    Helixi176 – 1838
    Beta strandi186 – 1883
    Helixi193 – 20614
    Helixi207 – 2093
    Helixi211 – 2144
    Beta strandi215 – 2184
    Turni219 – 2213
    Beta strandi222 – 2254

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B00X-ray1.88A/B1-127[»]
    1GXPX-ray2.50A/B/E/F124-229[»]
    1GXQX-ray2.00A124-229[»]
    1QQINMR-A126-229[»]
    1ZESX-ray1.90A/B/C1-125[»]
    2IYNX-ray2.08A/B/C1-127[»]
    2JB9X-ray1.70A/B1-127[»]
    2JBAX-ray1.45A/B1-127[»]
    2Z33NMR-A126-229[»]
    3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]
    ProteinModelPortaliP0AFJ5.
    SMRiP0AFJ5. Positions 2-123, 125-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AFJ5.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 120120Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034819.
    KOiK07657.
    OMAiSHPERAY.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP0AFJ5.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR011879. Sig_transdc_resp-reg_PhoB.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    TIGRFAMsiTIGR02154. PhoB. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AFJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI    50
    LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD 100
    DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE 150
    EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR 200
    RLRKALEPGG HDRMVQTVRG TGYRFSTRF 229
    Length:229
    Mass (Da):26,433
    Last modified:August 1, 1988 - v1
    Checksum:iEB46BA282F1F2C23
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04026 Genomic DNA. Translation: CAA27659.1.
    U73857 Genomic DNA. Translation: AAB18123.1.
    U00096 Genomic DNA. Translation: AAC73502.1.
    AP009048 Genomic DNA. Translation: BAE76179.1.
    PIRiA24256. RGECFB.
    RefSeqiNP_414933.1. NC_000913.3.
    YP_488691.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73502; AAC73502; b0399.
    BAE76179; BAE76179; BAE76179.
    GeneIDi12930822.
    945046.
    KEGGiecj:Y75_p0387.
    eco:b0399.
    PATRICi32115943. VBIEscCol129921_0413.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04026 Genomic DNA. Translation: CAA27659.1 .
    U73857 Genomic DNA. Translation: AAB18123.1 .
    U00096 Genomic DNA. Translation: AAC73502.1 .
    AP009048 Genomic DNA. Translation: BAE76179.1 .
    PIRi A24256. RGECFB.
    RefSeqi NP_414933.1. NC_000913.3.
    YP_488691.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B00 X-ray 1.88 A/B 1-127 [» ]
    1GXP X-ray 2.50 A/B/E/F 124-229 [» ]
    1GXQ X-ray 2.00 A 124-229 [» ]
    1QQI NMR - A 126-229 [» ]
    1ZES X-ray 1.90 A/B/C 1-125 [» ]
    2IYN X-ray 2.08 A/B/C 1-127 [» ]
    2JB9 X-ray 1.70 A/B 1-127 [» ]
    2JBA X-ray 1.45 A/B 1-127 [» ]
    2Z33 NMR - A 126-229 [» ]
    3T72 X-ray 4.33 1/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l 128-229 [» ]
    ProteinModelPortali P0AFJ5.
    SMRi P0AFJ5. Positions 2-123, 125-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35852N.
    IntActi P0AFJ5. 24 interactions.
    MINTi MINT-1313174.
    STRINGi 511145.b0399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73502 ; AAC73502 ; b0399 .
    BAE76179 ; BAE76179 ; BAE76179 .
    GeneIDi 12930822.
    945046.
    KEGGi ecj:Y75_p0387.
    eco:b0399.
    PATRICi 32115943. VBIEscCol129921_0413.

    Organism-specific databases

    EchoBASEi EB0721.
    EcoGenei EG10728. phoB.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034819.
    KOi K07657.
    OMAi SHPERAY.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P0AFJ5.

    Enzyme and pathway databases

    BioCyci EcoCyc:PHOB-MONOMER.
    ECOL316407:JW0389-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AFJ5.
    PROi P0AFJ5.

    Gene expression databases

    Genevestigatori P0AFJ5.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR011879. Sig_transdc_resp-reg_PhoB.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    TIGRFAMsi TIGR02154. PhoB. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12."
      Makino K., Shinagawa H., Amemura M., Nakata A.
      J. Mol. Biol. 190:37-44(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Three-dimensional crystal structure of the transcription factor PhoB receiver domain."
      Sola M., Gomis-Rueth F.-X., Serrano L., Gonzalez A., Coll M.
      J. Mol. Biol. 285:675-687(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-126, PHOSPHORYLATION AT ASP-53.
    6. "Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box."
      Okamura H., Hanaoka S., Nagadoi A., Makino K., Nishimura Y.
      J. Mol. Biol. 295:1225-1236(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 126-229.

    Entry informationi

    Entry nameiPHOB_ECOLI
    AccessioniPrimary (citable) accession number: P0AFJ5
    Secondary accession number(s): P08402, Q2MC27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3