Phosphate regulon transcriptional regulatory protein PhoB

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P0AFJ5 (PHOB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphate regulon transcriptional regulatory protein PhoB

Gene names

Name:	phoB
Ordered Locus Names:	b0399, JW0389

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated by PhoR or CreC.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Phosphate transport Transcription Transcription regulation Transport Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC phosphate ion transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of DNA-dependent transcription, initiation Inferred from direct assay PubMed 8648643. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW bacterial-type RNA polymerase holo enzyme binding Inferred from mutant phenotype PubMed 8648643. Source: EcoCyc phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-1116564,EBI-1116564

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 229

229

Phosphate regulon transcriptional regulatory protein PhoB

PRO_0000081186

Regions

Domain

1 – 120

120

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate

Secondary structure

229

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFJ5 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: EB46BA282F1F2C23
Show »

FASTA

229

26,433

        10         20         30         40         50         60 
MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI LLDWMLPGGS 

        70         80         90        100        110        120 
GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD DYITKPFSPK ELVARIKAVM 

       130        140        150        160        170        180 
RRISPMAVEE VIEMQGLSLD PTSHRVMAGE EPLEMGPTEF KLLHFFMTHP ERVYSREQLL 

       190        200        210        220 
NHVWGTNVYV EDRTVDVHIR RLRKALEPGG HDRMVQTVRG TGYRFSTRF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12." Makino K., Shinagawa H., Amemura M., Nakata A. J. Mol. Biol. 190:37-44(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Three-dimensional crystal structure of the transcription factor PhoB receiver domain." Sola M., Gomis-Rueth F.-X., Serrano L., Gonzalez A., Coll M. J. Mol. Biol. 285:675-687(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-126.
[6]	"Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box." Okamura H., Hanaoka S., Nagadoi A., Makino K., Nishimura Y. J. Mol. Biol. 295:1225-1236(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 126-229.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04026 Genomic DNA. Translation: CAA27659.1.
U73857 Genomic DNA. Translation: AAB18123.1.
U00096 Genomic DNA. Translation: AAC73502.1.
AP009048 Genomic DNA. Translation: BAE76179.1.

PIR

RGECFB. A24256.

RefSeq

NP_414933.1. NC_000913.2.
YP_488691.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B00	X-ray	1.88	A/B	1-127	[»]
1GXP	X-ray	2.50	A/B/E/F	124-229	[»]
1GXQ	X-ray	2.00	A	124-229	[»]
1QQI	NMR	-	A	126-229	[»]
1ZES	X-ray	1.90	A/B/C	1-124	[»]
2IYN	X-ray	2.08	A/B/C	1-127	[»]
2JB9	X-ray	1.70	A/B	1-127	[»]
2JBA	X-ray	1.45	A/B	1-127	[»]
2Z33	NMR	-	A	126-229	[»]
3T72	X-ray	4.33	1/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l	128-229	[»]

ProteinModelPortal

P0AFJ5.

SMR

P0AFJ5. Positions 2-123, 125-229.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35852N.

IntAct

P0AFJ5. 20 interactions.

MINT

MINT-1313174.

STRING

511145.b0399.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73502; AAC73502; b0399.
BAE76179; BAE76179; BAE76179.

GeneID

12930822.
945046.

KEGG

ecj:Y75_p0387.
eco:b0399.

PATRIC

32115943. VBIEscCol129921_0413.

Organism-specific databases

EchoBASE

EB0721.

EcoGene

EG10728. phoB.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034819.

K07657.

OMA

QVLEMGP.

ProtClustDB

PRK10161.

Enzyme and pathway databases

BioCyc

EcoCyc:PHOB-MONOMER.
ECOL316407:JW0389-MONOMER.

Gene expression databases

Genevestigator

P0AFJ5.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR011879. Sig_transdc_resp-reg_PhoB.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

TIGRFAMs

TIGR02154. PhoB. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0AFJ5.

Entry information

Entry name

PHOB_ECOLI

Accession

Primary (citable) accession number: P0AFJ5
Secondary accession number(s): P08402, Q2MC27

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents