Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-alanyl-D-alanine endopeptidase

Gene

pbpG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyzes the DD-diaminopimelate-alanine bonds in high-molecular-mass murein sacculi.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Acyl-ester intermediateBy similarity
Active sitei70 – 701Proton acceptorBy similarity
Active sitei124 – 1241By similarity
Binding sitei231 – 2311SubstrateBy similarity

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • serine-type D-Ala-D-Ala carboxypeptidase activity Source: GO_Central

GO - Biological processi

  • cell wall organization Source: EcoCyc
  • FtsZ-dependent cytokinesis Source: EcoCyc
  • peptidoglycan biosynthetic process Source: GO_Central
  • peptidoglycan metabolic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
  • response to drug Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12015-MONOMER.
ECOL316407:JW5355-MONOMER.
MetaCyc:EG12015-MONOMER.

Protein family/group databases

MEROPSiS11.002.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanyl-D-alanine endopeptidase (EC:3.4.21.-)
Short name:
DD-endopeptidase
Alternative name(s):
Penicillin-binding protein 7
Short name:
PBP-7
Gene namesi
Name:pbpG
Synonyms:yohB
Ordered Locus Names:b2134, JW5355
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12015. pbpG.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry

DrugBankiDB01331. Cefoxitin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 310285D-alanyl-D-alanine endopeptidasePRO_0000027237Add
BLAST

Post-translational modificationi

Pbp8 is a proteolytic product of Pbp7.

Proteomic databases

PaxDbiP0AFI5.

Interactioni

Protein-protein interaction databases

BioGridi4260453. 227 interactions.
DIPiDIP-48108N.
IntActiP0AFI5. 8 interactions.
STRINGi511145.b2134.

Structurei

3D structure databases

ProteinModelPortaliP0AFI5.
SMRiP0AFI5. Positions 38-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S11 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG41061WM. Bacteria.
COG1686. LUCA.
HOGENOMiHOG000199620.
InParanoidiP0AFI5.
KOiK07262.
OMAiTIYLALQ.
PhylomeDBiP0AFI5.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR018044. Peptidase_S11.
IPR001967. Peptidase_S11_N.
[Graphical view]
PfamiPF00768. Peptidase_S11. 1 hit.
[Graphical view]
PRINTSiPR00725. DADACBPTASE1.
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKFRVSLFS LALMLAVPFA PQAVAKTAAA TTASQPEIAS GSAMIVDLNT
60 70 80 90 100
NKVIYSNHPD LVRPIASISK LMTAMVVLDA RLPLDEKLKV DISQTPEMKG
110 120 130 140 150
VYSRVRLNSE ISRKDMLLLA LMSSENRAAA SLAHHYPGGY KAFIKAMNAK
160 170 180 190 200
AKSLGMNNTR FVEPTGLSVH NVSTARDLTK LLIASKQYPL IGQLSTTRED
210 220 230 240 250
MATFSNPTYT LPFRNTNHLV YRDNWNIQLT KTGFTNAAGH CLVMRTVINN
260 270 280 290 300
KPVALVVMDA FGKYTHFADA SRLRTWIETG KVMPVPAAAL SYKKQKAAQM
310
AAAGQTAQND
Length:310
Mass (Da):33,887
Last modified:December 4, 2007 - v2
Checksum:i4CD97290757526EE
GO

Sequence cautioni

The sequence AAA60496 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60496.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75195.2.
AP009048 Genomic DNA. Translation: BAE76611.1.
X01067 Genomic DNA. Translation: CAA25532.1.
PIRiE64981.
RefSeqiNP_416638.4. NC_000913.3.
WP_001319943.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75195; AAC75195; b2134.
BAE76611; BAE76611; BAE76611.
GeneIDi946662.
KEGGiecj:JW5355.
eco:b2134.
PATRICi32119609. VBIEscCol129921_2215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60496.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75195.2.
AP009048 Genomic DNA. Translation: BAE76611.1.
X01067 Genomic DNA. Translation: CAA25532.1.
PIRiE64981.
RefSeqiNP_416638.4. NC_000913.3.
WP_001319943.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFI5.
SMRiP0AFI5. Positions 38-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260453. 227 interactions.
DIPiDIP-48108N.
IntActiP0AFI5. 8 interactions.
STRINGi511145.b2134.

Chemistry

DrugBankiDB01331. Cefoxitin.

Protein family/group databases

MEROPSiS11.002.

Proteomic databases

PaxDbiP0AFI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75195; AAC75195; b2134.
BAE76611; BAE76611; BAE76611.
GeneIDi946662.
KEGGiecj:JW5355.
eco:b2134.
PATRICi32119609. VBIEscCol129921_2215.

Organism-specific databases

EchoBASEiEB1952.
EcoGeneiEG12015. pbpG.

Phylogenomic databases

eggNOGiENOG41061WM. Bacteria.
COG1686. LUCA.
HOGENOMiHOG000199620.
InParanoidiP0AFI5.
KOiK07262.
OMAiTIYLALQ.
PhylomeDBiP0AFI5.

Enzyme and pathway databases

BioCyciEcoCyc:EG12015-MONOMER.
ECOL316407:JW5355-MONOMER.
MetaCyc:EG12015-MONOMER.

Miscellaneous databases

PROiP0AFI5.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR018044. Peptidase_S11.
IPR001967. Peptidase_S11_N.
[Graphical view]
PfamiPF00768. Peptidase_S11. 1 hit.
[Graphical view]
PRINTSiPR00725. DADACBPTASE1.
SUPFAMiSSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPBP7_ECOLI
AccessioniPrimary (citable) accession number: P0AFI5
Secondary accession number(s): P33364, Q2MAU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In E.coli there are three murein endopeptidases: two are penicillin sensitive (DacB and PbpG), the other (MepA) not.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.