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Protein

D-alanyl-D-alanine endopeptidase

Gene

pbpG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyzes the DD-diaminopimelate-alanine bonds in high-molecular-mass murein sacculi.

Miscellaneous

In E.coli there are three murein endopeptidases: two are penicillin sensitive (DacB and PbpG), the other (MepA) not.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Acyl-ester intermediateBy similarity1
Active sitei70Proton acceptorBy similarity1
Active sitei124By similarity1
Binding sitei231SubstrateBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • serine-type D-Ala-D-Ala carboxypeptidase activity Source: InterPro

GO - Biological processi

  • cell wall organization Source: EcoCyc
  • FtsZ-dependent cytokinesis Source: EcoCyc
  • peptidoglycan biosynthetic process Source: UniProtKB-KW
  • peptidoglycan metabolic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
  • response to drug Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12015-MONOMER
MetaCyc:EG12015-MONOMER

Protein family/group databases

MEROPSiS11.002

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanyl-D-alanine endopeptidase (EC:3.4.21.-)
Short name:
DD-endopeptidase
Alternative name(s):
Penicillin-binding protein 7
Short name:
PBP-7
Gene namesi
Name:pbpG
Synonyms:yohB
Ordered Locus Names:b2134, JW5355
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12015 pbpG

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB01331 Cefoxitin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000002723726 – 310D-alanyl-D-alanine endopeptidaseAdd BLAST285

Post-translational modificationi

Pbp8 is a proteolytic product of Pbp7.

Proteomic databases

PaxDbiP0AFI5
PRIDEiP0AFI5

Interactioni

Protein-protein interaction databases

BioGridi4260453, 247 interactors
DIPiDIP-48108N
IntActiP0AFI5, 9 interactors
STRINGi316385.ECDH10B_2290

Structurei

3D structure databases

ProteinModelPortaliP0AFI5
SMRiP0AFI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S11 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG41061WM Bacteria
COG1686 LUCA
HOGENOMiHOG000199620
InParanoidiP0AFI5
KOiK07262
OMAiYNLVFVN
PhylomeDBiP0AFI5

Family and domain databases

InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR018044 Peptidase_S11
IPR001967 Peptidase_S11_N
PfamiView protein in Pfam
PF00768 Peptidase_S11, 1 hit
PRINTSiPR00725 DADACBPTASE1
SUPFAMiSSF56601 SSF56601, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKFRVSLFS LALMLAVPFA PQAVAKTAAA TTASQPEIAS GSAMIVDLNT
60 70 80 90 100
NKVIYSNHPD LVRPIASISK LMTAMVVLDA RLPLDEKLKV DISQTPEMKG
110 120 130 140 150
VYSRVRLNSE ISRKDMLLLA LMSSENRAAA SLAHHYPGGY KAFIKAMNAK
160 170 180 190 200
AKSLGMNNTR FVEPTGLSVH NVSTARDLTK LLIASKQYPL IGQLSTTRED
210 220 230 240 250
MATFSNPTYT LPFRNTNHLV YRDNWNIQLT KTGFTNAAGH CLVMRTVINN
260 270 280 290 300
KPVALVVMDA FGKYTHFADA SRLRTWIETG KVMPVPAAAL SYKKQKAAQM
310
AAAGQTAQND
Length:310
Mass (Da):33,887
Last modified:December 4, 2007 - v2
Checksum:i4CD97290757526EE
GO

Sequence cautioni

The sequence AAA60496 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA Translation: AAA60496.1 Different initiation.
U00096 Genomic DNA Translation: AAC75195.2
AP009048 Genomic DNA Translation: BAE76611.1
X01067 Genomic DNA Translation: CAA25532.1
PIRiE64981
RefSeqiNP_416638.4, NC_000913.3
WP_001319943.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75195; AAC75195; b2134
BAE76611; BAE76611; BAE76611
GeneIDi946662
KEGGiecj:JW5355
eco:b2134
PATRICifig|1411691.4.peg.109

Similar proteinsi

Entry informationi

Entry nameiPBP7_ECOLI
AccessioniPrimary (citable) accession number: P0AFI5
Secondary accession number(s): P33364, Q2MAU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health