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Protein

DNA topoisomerase 4 subunit A

Gene

parC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322). It relaxes supercoiled DNA (PubMed:12269820, PuMed:16023670, PubMed:21300644). MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation (PubMed:20921377).5 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation3 Publications

Enzyme regulationi

Inhibited by quinolones.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Transition state stabilizerUniRule annotation1
Active sitei120O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

  • ATP binding Source: InterPro
  • DNA binding Source: EcoliWiki
  • DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  • chromosome segregation Source: EcoliWiki
  • DNA topological change Source: EcoliWiki
  • plasmid partitioning Source: EcoliWiki
  • sister chromatid cohesion Source: EcoliWiki

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Enzyme and pathway databases

BioCyciEcoCyc:EG10686-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit AUniRule annotation
Gene namesi
Name:parCUniRule annotation
Ordered Locus Names:b3019, JW2987
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10686 parC

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: InterPro
  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • DNA topoisomerase complex (ATP-hydrolyzing) Source: EcoliWiki
  • extrinsic component of plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi80S → L: Confers resistance to quinolones. No effect on catalytic activity. 1 Publication1
Mutagenesisi84E → K: Strongly reduced enzyme activity. Increases stability of covalent reaction intermediate with DNA. Confers resistance to quinolones. 1 Publication1
Mutagenesisi84E → P: Confers resistance to quinolones. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1895
DrugBankiDB00817 Rosoxacin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001453971 – 752DNA topoisomerase 4 subunit AAdd BLAST752

Proteomic databases

EPDiP0AFI2
PaxDbiP0AFI2
PRIDEiP0AFI2

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE. Interacts with MukB.UniRule annotation4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39Interaction with DNAUniRule annotation1
Sitei75Interaction with DNAUniRule annotation1
Sitei77Interaction with DNAUniRule annotation1

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4262365, 18 interactors
DIPiDIP-36030N
IntActiP0AFI2, 24 interactors
MINTiP0AFI2
STRINGi316385.ECDH10B_3193

Chemistry databases

BindingDBiP0AFI2

Structurei

Secondary structure

1752
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni34 – 36Combined sources3
Helixi40 – 49Combined sources10
Helixi67 – 71Combined sources5
Turni72 – 74Combined sources3
Helixi80 – 89Combined sources10
Beta strandi99 – 102Combined sources4
Beta strandi107 – 111Combined sources5
Helixi120 – 122Combined sources3
Beta strandi123 – 125Combined sources3
Helixi127 – 129Combined sources3
Helixi130 – 133Combined sources4
Turni134 – 137Combined sources4
Beta strandi143 – 145Combined sources3
Beta strandi149 – 156Combined sources8
Helixi163 – 167Combined sources5
Helixi185 – 197Combined sources13
Helixi203 – 206Combined sources4
Turni207 – 209Combined sources3
Beta strandi216 – 221Combined sources6
Helixi227 – 234Combined sources8
Beta strandi236 – 242Combined sources7
Beta strandi244 – 248Combined sources5
Beta strandi251 – 256Combined sources6
Helixi263 – 275Combined sources13
Beta strandi282 – 287Combined sources6
Beta strandi291 – 293Combined sources3
Beta strandi297 – 305Combined sources9
Helixi308 – 318Combined sources11
Beta strandi322 – 328Combined sources7
Beta strandi330 – 332Combined sources3
Beta strandi338 – 340Combined sources3
Helixi343 – 384Combined sources42
Helixi386 – 395Combined sources10
Beta strandi396 – 398Combined sources3
Helixi399 – 405Combined sources7
Helixi413 – 418Combined sources6
Helixi422 – 425Combined sources4
Helixi427 – 452Combined sources26
Helixi454 – 472Combined sources19
Beta strandi499 – 505Combined sources7
Beta strandi508 – 516Combined sources9
Beta strandi520 – 523Combined sources4
Beta strandi531 – 538Combined sources8
Beta strandi539 – 541Combined sources3
Beta strandi543 – 547Combined sources5
Beta strandi550 – 555Combined sources6
Helixi557 – 559Combined sources3
Beta strandi563 – 565Combined sources3
Helixi570 – 572Combined sources3
Beta strandi582 – 586Combined sources5
Beta strandi593 – 598Combined sources6
Beta strandi601 – 607Combined sources7
Helixi608 – 611Combined sources4
Beta strandi642 – 647Combined sources6
Beta strandi650 – 656Combined sources7
Helixi657 – 659Combined sources3
Beta strandi664 – 667Combined sources4
Beta strandi669 – 672Combined sources4
Helixi676 – 680Combined sources5
Beta strandi686 – 692Combined sources7
Beta strandi697 – 702Combined sources6
Beta strandi705 – 709Combined sources5
Helixi711 – 715Combined sources5
Beta strandi736 – 740Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZVTX-ray1.70A/B497-752[»]
1ZVUX-ray3.00A27-742[»]
4MN4X-ray2.30A/B497-752[»]
ProteinModelPortaliP0AFI2
SMRiP0AFI2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFI2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni497 – 752Sufficient for MukB bindingAdd BLAST256

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24 Bacteria
COG0188 LUCA
HOGENOMiHOG000076277
InParanoidiP0AFI2
KOiK02621
OMAiPRSNRID
PhylomeDBiP0AFI2

Family and domain databases

Gene3Di1.10.268.10, 1 hit
2.120.10.90, 1 hit
3.90.199.10, 1 hit
HAMAPiMF_00936 ParC_type1, 1 hit
InterProiView protein in InterPro
IPR006691 GyrA/parC_rep
IPR035516 Gyrase/topoIV_suA_C
IPR013760 Topo_IIA-like_dom_sf
IPR002205 Topo_IIA_A/C
IPR013758 Topo_IIA_A/C_ab
IPR013757 Topo_IIA_A_a_sf
IPR005742 TopoIV_A_Gneg
PfamiView protein in Pfam
PF03989 DNA_gyraseA_C, 2 hits
PF00521 DNA_topoisoIV, 1 hit
SMARTiView protein in SMART
SM00434 TOP4c, 1 hit
SUPFAMiSSF101904 SSF101904, 1 hit
SSF56719 SSF56719, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS
60 70 80 90 100
ELGLNASAKF KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV
110 120 130 140 150
DGQGNWGAPD DPKSFAAMRY TESRLSKYSE LLLSELGQGT ADWVPNFDGT
160 170 180 190 200
LQEPKMLPAR LPNILLNGTT GIAVGMATDI PPHNLREVAQ AAIALIDQPK
210 220 230 240 250
TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR MRAVWKKEDG
260 270 280 290 300
AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV
310 320 330 340 350
PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW
360 370 380 390 400
LVFRRDTVRR RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK
410 420 430 440 450
PALMSRFGLT ETQAEAILEL KLRHLAKLEE MKIRGEQSEL EKERDQLQGI
460 470 480 490 500
LASERKMNNL LKKELQADAQ AYGDDRRSPL QEREEAKAMS EHDMLPSEPV
510 520 530 540 550
TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN QPVVFVDSTG
560 570 580 590 600
RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA
610 620 630 640 650
GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG
660 670 680 690 700
RMLMFPVSDL PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI
710 720 730 740 750
HVGKRKIKLR PEELQKVTGE RGRRGTLMRG LQRIDRVEID SPRRASSGDS

EE
Length:752
Mass (Da):83,831
Last modified:December 20, 2005 - v1
Checksum:i0D4907E96CEE7086
GO

Sequence cautioni

The sequence AAA24297 differs from that shown. Reason: Frameshift at position 10.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58408 Genomic DNA Translation: AAA24297.1 Frameshift.
M63491 Genomic DNA Translation: AAA24396.1
U28377 Genomic DNA Translation: AAA69187.1
U00096 Genomic DNA Translation: AAC76055.1
AP009048 Genomic DNA Translation: BAE77075.1
L22025 Unassigned DNA Translation: AAC36840.1
PIRiA65089
RefSeqiNP_417491.1, NC_000913.3
WP_001281881.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76055; AAC76055; b3019
BAE77075; BAE77075; BAE77075
GeneIDi947499
KEGGiecj:JW2987
eco:b3019
PATRICifig|1411691.4.peg.3711

Similar proteinsi

Entry informationi

Entry nameiPARC_ECOLI
AccessioniPrimary (citable) accession number: P0AFI2
Secondary accession number(s): O69154, P20082, Q2M9I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health