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P0AFI2

- PARC_ECOLI

UniProt

P0AFI2 - PARC_ECOLI

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Protein
DNA topoisomerase 4 subunit A
Gene
parC, b3019, JW2987
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.4 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.3 Publications

Enzyme regulationi

Inhibited by quinolones.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei39 – 391Interaction with DNA By similarity
Sitei75 – 751Interaction with DNA By similarity
Sitei77 – 771Interaction with DNA By similarity
Sitei119 – 1191Transition state stabilizer By similarity
Active sitei120 – 1201O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. DNA binding Source: EcoliWiki
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. protein binding Source: IntAct

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. DNA topological change Source: EcoliWiki
  3. DNA unwinding involved in DNA replication Source: RefGenome
  4. chromosome segregation Source: EcoliWiki
  5. plasmid partitioning Source: EcoliWiki
  6. sister chromatid cohesion Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10686-MONOMER.
ECOL316407:JW2987-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit A (EC:5.99.1.3)
Alternative name(s):
Topoisomerase IV subunit A
Gene namesi
Name:parC
Ordered Locus Names:b3019, JW2987
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10686. parC.

Subcellular locationi

GO - Cellular componenti

  1. DNA topoisomerase complex (ATP-hydrolyzing) Source: EcoliWiki
  2. chromosome Source: InterPro
  3. cytoplasm Source: EcoliWiki
  4. extrinsic component of plasma membrane Source: EcoliWiki
  5. nucleoid Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi80 – 801S → L: Confers resistance to quinolones. No effect on catalytic activity. 1 Publication
Mutagenesisi84 – 841E → K: Strongly reduced enzyme activity. Increases stability of covalent reaction intermediate with DNA. Confers resistance to quinolones. 1 Publication
Mutagenesisi84 – 841E → P: Confers resistance to quinolones. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752DNA topoisomerase 4 subunit AUniRule annotation
PRO_0000145397Add
BLAST

Proteomic databases

PaxDbiP0AFI2.
PRIDEiP0AFI2.

Expressioni

Gene expression databases

GenevestigatoriP0AFI2.

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE. Interacts with MukB.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mukBP225237EBI-878544,EBI-542943

Protein-protein interaction databases

DIPiDIP-36030N.
IntActiP0AFI2. 23 interactions.
MINTiMINT-1227290.
STRINGi511145.b3019.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni34 – 363
Helixi40 – 4910
Helixi67 – 715
Turni72 – 743
Helixi80 – 8910
Beta strandi99 – 1024
Beta strandi107 – 1115
Helixi120 – 1223
Beta strandi123 – 1253
Helixi127 – 1293
Helixi130 – 1334
Turni134 – 1374
Beta strandi143 – 1453
Beta strandi149 – 1568
Helixi163 – 1675
Helixi185 – 19713
Helixi203 – 2064
Turni207 – 2093
Beta strandi216 – 2216
Helixi227 – 2348
Beta strandi236 – 2427
Beta strandi244 – 2485
Beta strandi251 – 2566
Helixi263 – 27513
Beta strandi282 – 2876
Beta strandi291 – 2933
Beta strandi297 – 3059
Helixi308 – 31811
Beta strandi322 – 3287
Beta strandi330 – 3323
Beta strandi338 – 3403
Helixi343 – 38442
Helixi386 – 39510
Beta strandi396 – 3983
Helixi399 – 4057
Helixi413 – 4186
Helixi422 – 4254
Helixi427 – 45226
Helixi454 – 47219
Beta strandi499 – 5057
Beta strandi508 – 5169
Beta strandi520 – 5234
Beta strandi531 – 5388
Beta strandi539 – 5413
Beta strandi543 – 5475
Beta strandi550 – 5556
Helixi557 – 5593
Beta strandi563 – 5653
Helixi570 – 5723
Beta strandi582 – 5865
Beta strandi593 – 5986
Beta strandi601 – 6077
Helixi608 – 6114
Beta strandi642 – 6476
Beta strandi650 – 6567
Helixi657 – 6593
Beta strandi664 – 6674
Beta strandi669 – 6724
Helixi676 – 6805
Beta strandi686 – 6927
Beta strandi697 – 7026
Beta strandi705 – 7095
Helixi711 – 7155
Beta strandi736 – 7405

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZVTX-ray1.70A/B497-752[»]
1ZVUX-ray3.00A27-742[»]
4MN4X-ray2.30A/B497-752[»]
ProteinModelPortaliP0AFI2.
SMRiP0AFI2. Positions 28-742.

Miscellaneous databases

EvolutionaryTraceiP0AFI2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni497 – 752256Sufficient for MukB bindingUniRule annotation
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0188.
HOGENOMiHOG000076277.
KOiK02621.
OMAiILSEMGW.
OrthoDBiEOG661H5V.
PhylomeDBiP0AFI2.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00936. ParC_type1.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
IPR005742. TopoIV_A_Gneg.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 2 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFI2-1 [UniParc]FASTAAdd to Basket

« Hide

MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS    50
ELGLNASAKF KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV 100
DGQGNWGAPD DPKSFAAMRY TESRLSKYSE LLLSELGQGT ADWVPNFDGT 150
LQEPKMLPAR LPNILLNGTT GIAVGMATDI PPHNLREVAQ AAIALIDQPK 200
TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR MRAVWKKEDG 250
AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV 300
PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW 350
LVFRRDTVRR RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK 400
PALMSRFGLT ETQAEAILEL KLRHLAKLEE MKIRGEQSEL EKERDQLQGI 450
LASERKMNNL LKKELQADAQ AYGDDRRSPL QEREEAKAMS EHDMLPSEPV 500
TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN QPVVFVDSTG 550
RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA 600
GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG 650
RMLMFPVSDL PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI 700
HVGKRKIKLR PEELQKVTGE RGRRGTLMRG LQRIDRVEID SPRRASSGDS 750
EE 752
Length:752
Mass (Da):83,831
Last modified:December 20, 2005 - v1
Checksum:i0D4907E96CEE7086
GO

Sequence cautioni

The sequence AAA24297.1 differs from that shown. Reason: Frameshift at position 10.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58408 Genomic DNA. Translation: AAA24297.1. Frameshift.
M63491 Genomic DNA. Translation: AAA24396.1.
U28377 Genomic DNA. Translation: AAA69187.1.
U00096 Genomic DNA. Translation: AAC76055.1.
AP009048 Genomic DNA. Translation: BAE77075.1.
L22025 Unassigned DNA. Translation: AAC36840.1.
PIRiA65089.
RefSeqiNP_417491.1. NC_000913.3.
YP_491211.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76055; AAC76055; b3019.
BAE77075; BAE77075; BAE77075.
GeneIDi12933371.
947499.
KEGGiecj:Y75_p2945.
eco:b3019.
PATRICi32121452. VBIEscCol129921_3113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58408 Genomic DNA. Translation: AAA24297.1 . Frameshift.
M63491 Genomic DNA. Translation: AAA24396.1 .
U28377 Genomic DNA. Translation: AAA69187.1 .
U00096 Genomic DNA. Translation: AAC76055.1 .
AP009048 Genomic DNA. Translation: BAE77075.1 .
L22025 Unassigned DNA. Translation: AAC36840.1 .
PIRi A65089.
RefSeqi NP_417491.1. NC_000913.3.
YP_491211.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZVT X-ray 1.70 A/B 497-752 [» ]
1ZVU X-ray 3.00 A 27-742 [» ]
4MN4 X-ray 2.30 A/B 497-752 [» ]
ProteinModelPortali P0AFI2.
SMRi P0AFI2. Positions 28-742.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36030N.
IntActi P0AFI2. 23 interactions.
MINTi MINT-1227290.
STRINGi 511145.b3019.

Chemistry

BindingDBi P0AFI2.
ChEMBLi CHEMBL2363076.
DrugBanki DB00817. Rosoxacin.

Proteomic databases

PaxDbi P0AFI2.
PRIDEi P0AFI2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76055 ; AAC76055 ; b3019 .
BAE77075 ; BAE77075 ; BAE77075 .
GeneIDi 12933371.
947499.
KEGGi ecj:Y75_p2945.
eco:b3019.
PATRICi 32121452. VBIEscCol129921_3113.

Organism-specific databases

EchoBASEi EB0680.
EcoGenei EG10686. parC.

Phylogenomic databases

eggNOGi COG0188.
HOGENOMi HOG000076277.
KOi K02621.
OMAi ILSEMGW.
OrthoDBi EOG661H5V.
PhylomeDBi P0AFI2.

Enzyme and pathway databases

BioCyci EcoCyc:EG10686-MONOMER.
ECOL316407:JW2987-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFI2.
PROi P0AFI2.

Gene expression databases

Genevestigatori P0AFI2.

Family and domain databases

Gene3Di 3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPi MF_00936. ParC_type1.
InterProi IPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
IPR005742. TopoIV_A_Gneg.
[Graphical view ]
Pfami PF03989. DNA_gyraseA_C. 2 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view ]
SMARTi SM00434. TOP4c. 1 hit.
[Graphical view ]
SUPFAMi SSF56719. SSF56719. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "New topoisomerase essential for chromosome segregation in E. coli."
    Kato J., Nishimura Y., Imamura R., Niki H., Hiraga S., Suzuki H.
    Cell 63:393-404(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Erratum
    Kato J., Nishimura Y., Imamura R., Niki H., Hiraga S., Suzuki H.
    Cell 65:1289-1290(1991)
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction."
    Li Y., Stewart N.K., Berger A.J., Vos S., Schoeffler A.J., Berger J.M., Chait B.T., Oakley M.G.
    Proc. Natl. Acad. Sci. U.S.A. 107:18832-18837(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-43; 97-113; 201-225 AND 552-564, FUNCTION MODIFIED BY MUKB, INTERACTION WITH MUKB.
  6. "Characterization of the Escherichia coli gene for 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC)."
    Coleman J.
    Mol. Gen. Genet. 232:295-303(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 724-752.
  7. "Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions."
    Peng H., Marians K.J.
    J. Biol. Chem. 268:24481-24490(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION, SUBUNIT, CHARACTERIZATION.
    Strain: K12.
  8. "The Glu-84 of the ParC subunit plays critical roles in both topoisomerase IV-quinolone and topoisomerase IV-DNA interactions."
    Hiasa H.
    Biochemistry 41:11779-11785(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-80 AND GLU-84, FUNCTION, ENZYME REGULATION, CATALYTIC ACTIVITY.
  9. "Use of divalent metal ions in the DNA cleavage reaction of topoisomerase IV."
    Pitts S.L., Liou G.F., Mitchenall L.A., Burgin A.B., Maxwell A., Neuman K.C., Osheroff N.
    Nucleic Acids Res. 39:4808-4817(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, CATALYTIC ACTIVITY.
  10. "The structural basis for substrate specificity in DNA topoisomerase IV."
    Corbett K.D., Schoeffler A.J., Thomsen N.D., Berger J.M.
    J. Mol. Biol. 351:545-561(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-752, FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH PARE, SUBUNIT.

Entry informationi

Entry nameiPARC_ECOLI
AccessioniPrimary (citable) accession number: P0AFI2
Secondary accession number(s): O69154, P20082, Q2M9I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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