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P0AFH8 (OSMY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Osmotically-inducible protein Y
Gene names
Name:osmY
Ordered Locus Names:b4376, JW4338
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length201 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Periplasm.

Induction

By elevated osmotic pressure in the growth medium.

Sequence similarities

Contains 2 BON domains.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainRepeat
Signal
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to osmotic stress

Inferred from mutant phenotype Ref.1. Source: EcoCyc

   Cellular_componentperiplasmic space

Inferred from direct assay Ref.1. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.1 Ref.6 Ref.7
Chain29 – 201173Osmotically-inducible protein Y
PRO_0000021953

Regions

Domain55 – 12369BON 1
Domain134 – 20168BON 2

Sequences

Sequence LengthMass (Da)Tools
P0AFH8 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: BF74D4388D984B93

FASTA20121,074
        10         20         30         40         50         60 
MTMTRLKISK TLLAVMLTSA VATGSAYAEN NAQTTNESAG QKVDSSMNKV GNFMDDSAIT 

        70         80         90        100        110        120 
AKVKAALVDH DNIKSTDISV KTDQKVVTLS GFVESQAQAE EAVKVAKGVE GVTSVSDKLH 

       130        140        150        160        170        180 
VRDAKEGSVK GYAGDTATTS EIKAKLLADD IVPSRHVKVE TTDGVVQLSG TVDSQAQSDR 

       190        200 
AESIAKAVDG VKSVKNDLKT K 

« Hide

References

« Hide 'large scale' references
[1]"osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli."
Yim H.H., Villarejo M.
J. Bacteriol. 174:3637-3644(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-43.
Strain: K12.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli."
Lange R., Barth M., Hengge-Aronis R.
J. Bacteriol. 175:7910-7917(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-40.
Strain: K12 / EMG2.
[7]"Protein identification with N and C-terminal sequence tags in proteome projects."
Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F.
J. Mol. Biol. 278:599-608(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-32.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M89635 Unassigned DNA. Translation: AAA16621.1.
U14003 Genomic DNA. Translation: AAA97272.1.
U00096 Genomic DNA. Translation: AAC77329.1.
AP009048 Genomic DNA. Translation: BAE78364.1.
S67278 Genomic DNA. Translation: AAP14005.1.
PIRA41899.
RefSeqNP_418793.1. NC_000913.3.
YP_492505.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AFH8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47968N.
IntActP0AFH8. 3 interactions.
STRING511145.b4376.

Proteomic databases

PaxDbP0AFH8.
PRIDEP0AFH8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77329; AAC77329; b4376.
BAE78364; BAE78364; BAE78364.
GeneID12933797.
948895.
KEGGecj:Y75_p4259.
eco:b4376.
PATRIC32124362. VBIEscCol129921_4521.

Organism-specific databases

EchoBASEEB1364.
EcoGeneEG11391. osmY.

Phylogenomic databases

eggNOGCOG2823.
HOGENOMHOG000127831.
KOK04065.
OMAGYMDDSG.
OrthoDBEOG6B3669.
PhylomeDBP0AFH8.

Enzyme and pathway databases

BioCycEcoCyc:EG11391-MONOMER.
ECOL316407:JW4338-MONOMER.

Gene expression databases

GenevestigatorP0AFH8.

Family and domain databases

InterProIPR007055. BON_dom.
IPR014004. Transpt-assoc_nodulatn_dom_bac.
[Graphical view]
PfamPF04972. BON. 2 hits.
[Graphical view]
SMARTSM00749. BON. 2 hits.
[Graphical view]
PROSITEPS50914. BON. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

PROP0AFH8.

Entry information

Entry nameOSMY_ECOLI
AccessionPrimary (citable) accession number: P0AFH8
Secondary accession number(s): P27291, Q2M5U2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene