P0AFH2 (OPPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligopeptide transport system permease protein oppB

Gene names

Name:	oppB
Ordered Locus Names:	b1244, JW1236

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Peptide transport Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oligopeptide-transporting ATPase activity Inferred from mutant phenotype. Source: EcoCyc tripeptide transporter activity Inferred from mutant phenotype. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Oligopeptide transport system permease protein oppB		PRO_0000060143
Regions
	Topological domain	1 – 9	9	Cytoplasmic Probable
	Transmembrane	10 – 30	21	Helical; Probable
	Topological domain	31 – 99	69	Extracellular Probable
	Transmembrane	100 – 121	22	Helical; Probable
	Topological domain	122 – 137	16	Cytoplasmic Probable
	Transmembrane	138 – 158	21	Helical; Probable
	Topological domain	159 – 172	14	Extracellular Probable
	Transmembrane	173 – 190	18	Helical; Probable
	Topological domain	191 – 226	36	Cytoplasmic Probable
	Transmembrane	227 – 250	24	Helical; Probable
	Topological domain	251 – 271	21	Extracellular Probable
	Transmembrane	272 – 293	22	Helical; Probable
	Topological domain	294 – 306	13	Cytoplasmic Probable
	Domain	94 – 293	200	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AFH2 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: F74579B3F9EA4615	FASTA	306	33,443
	10 20 30 40 50 60 MLKFILRRCL EAIPTLFILI TISFFMMRLA PGSPFTGERT LPPEVMANIE AKYHLNDPIM 70 80 90 100 110 120 TQYFSYLKQL AHGDFGPSFK YKDYSVNDLV ASSFPVSAKL GAAAFFLAVI LGVSAGVIAA 130 140 150 160 170 180 LKQNTKWDYT VMGLAMTGVV IPSFVVAPLL VMIFAIILHW LPGGGWNGGA LKFMILPMVA 190 200 210 220 230 240 LSLAYIASIA RITRGSMIEV LHSNFIRTAR AKGLPMRRII LRHALKPALL PVLSYMGPAF 250 260 270 280 290 300 VGIITGSMVI ETIYGLPGIG QLFVNGALNR DYSLVLSLTI LVGALTILFN AIVDVLYAVI DPKIRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of the polyamine-induced protein as a periplasmic oligopeptide binding protein." Kashiwagi K., Yamaguchi Y., Sakai Y., Kobayashi H., Igarashi K. J. Biol. Chem. 265:8387-8391(1990) [PubMed: 2187863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74326.1. AP009048 Genomic DNA. Translation: BAA14776.1. J05433 Genomic DNA. No translation available.
PIR	B36263. G64871.
RefSeq	NP_415760.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AFH2.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48074N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002310; EBESCP00000002310; EBESCG00000001885. EBESCT00000017296; EBESCP00000016587; EBESCG00000016353.
GeneID	945823.
GenomeReviews	Gene locus JW1236 in contig AP009048_GR. Gene locus b1244 in contig U00096_GR.
KEGG	ecj:JW1236. eco:b1244.
PATRIC	32117748. VBIEscCol129921_1294.
Organism-specific databases
EchoBASE	EB0669.
EcoGene	EG10675. oppB.
Phylogenomic databases
eggNOG	COG0601.
GeneTree	EBGT00070000031776.
HOGENOM	HBG724229.
OMA	FIFRRCL.
PhylomeDB	P0AFH2.
ProtClustDB	PRK09471.
Enzyme and pathway databases
BioCyc	EcoCyc:OPPB-MONOMER.
Gene expression databases
Genevestigator	P0AFH2.
Family and domain databases
InterPro	IPR000515. BPD_transp. [Graphical view]
KO	K15581.
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPPB_ECOLI

Accession

Primary (citable) accession number: P0AFH2
Secondary accession number(s): P31132, P76026, P77550

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program