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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Nucleophile; methyl group acceptorCurated1

GO - Molecular functioni

  • methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • DNA dealkylation involved in DNA repair Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG10668-MONOMER
MetaCyc:EG10668-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:b1335, JW1329
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10668 ogt

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102C → G: Little effect on the activity. 1 Publication1
Mutagenesisi139C → G: Eliminates the activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393651 – 171Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST171

Proteomic databases

PaxDbiP0AFH0
PRIDEiP0AFH0

Interactioni

Protein-protein interaction databases

BioGridi4262876, 110 interactors
DIPiDIP-48073N
IntActiP0AFH0, 6 interactors
STRINGi316385.ECDH10B_1455

Structurei

3D structure databases

ProteinModelPortaliP0AFH0
SMRiP0AFH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K85 Bacteria
COG0350 LUCA
HOGENOMiHOG000244137
InParanoidiP0AFH0
KOiK00567
OMAiTLGGFRY
PhylomeDBiP0AFH0

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR008332 MethylG_MeTrfase_N
IPR023546 MGMT
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
PF02870 Methyltransf_1N, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG
60 70 80 90 100
YERISATNPG GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI
110 120 130 140 150
PCGQVMHYGQ LAEQLGRPGA ARAVGAANGS NPISIVVPCH RVIGRNGTMT
160 170
GYAGGVQRKE WLLRHEGYLL L
Length:171
Mass (Da):19,179
Last modified:December 20, 2005 - v1
Checksum:i39EFBF1539F67D26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37R → A in CAA68548 (PubMed:2825131).Curated1
Sequence conflicti68E → D in CAA68548 (PubMed:2825131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00495 Genomic DNA Translation: CAA68548.1
U00096 Genomic DNA Translation: AAC74417.1
AP009048 Genomic DNA Translation: BAA14928.1
PIRiB64883 XUECAD
RefSeqiNP_415851.1, NC_000913.3
WP_000945011.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74417; AAC74417; b1335
BAA14928; BAA14928; BAA14928
GeneIDi945853
KEGGiecj:JW1329
eco:b1335
PATRICifig|1411691.4.peg.942

Similar proteinsi

Entry informationi

Entry nameiOGT_ECOLI
AccessioniPrimary (citable) accession number: P0AFH0
Secondary accession number(s): P09168, P78284
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health