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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Nucleophile; methyl group acceptorCurated1

GO - Molecular functioni

GO - Biological processi

  • DNA dealkylation involved in DNA repair Source: GO_Central
  • DNA repair Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG10668-MONOMER.
ECOL316407:JW1329-MONOMER.
MetaCyc:EG10668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:b1335, JW1329
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10668. ogt.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102C → G: Little effect on the activity. 1 Publication1
Mutagenesisi139C → G: Eliminates the activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393651 – 171Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST171

Proteomic databases

PaxDbiP0AFH0.
PRIDEiP0AFH0.

Interactioni

Protein-protein interaction databases

BioGridi4262876. 110 interactors.
DIPiDIP-48073N.
IntActiP0AFH0. 6 interactors.
MINTiMINT-1243838.
STRINGi511145.b1335.

Structurei

3D structure databases

ProteinModelPortaliP0AFH0.
SMRiP0AFH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K85. Bacteria.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiP0AFH0.
KOiK00567.
OMAiEDKIATP.
PhylomeDBiP0AFH0.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG
60 70 80 90 100
YERISATNPG GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI
110 120 130 140 150
PCGQVMHYGQ LAEQLGRPGA ARAVGAANGS NPISIVVPCH RVIGRNGTMT
160 170
GYAGGVQRKE WLLRHEGYLL L
Length:171
Mass (Da):19,179
Last modified:December 20, 2005 - v1
Checksum:i39EFBF1539F67D26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37R → A in CAA68548 (PubMed:2825131).Curated1
Sequence conflicti68E → D in CAA68548 (PubMed:2825131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00495 Genomic DNA. Translation: CAA68548.1.
U00096 Genomic DNA. Translation: AAC74417.1.
AP009048 Genomic DNA. Translation: BAA14928.1.
PIRiB64883. XUECAD.
RefSeqiNP_415851.1. NC_000913.3.
WP_000945011.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74417; AAC74417; b1335.
BAA14928; BAA14928; BAA14928.
GeneIDi945853.
KEGGiecj:JW1329.
eco:b1335.
PATRICi32117946. VBIEscCol129921_1393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00495 Genomic DNA. Translation: CAA68548.1.
U00096 Genomic DNA. Translation: AAC74417.1.
AP009048 Genomic DNA. Translation: BAA14928.1.
PIRiB64883. XUECAD.
RefSeqiNP_415851.1. NC_000913.3.
WP_000945011.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFH0.
SMRiP0AFH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262876. 110 interactors.
DIPiDIP-48073N.
IntActiP0AFH0. 6 interactors.
MINTiMINT-1243838.
STRINGi511145.b1335.

Proteomic databases

PaxDbiP0AFH0.
PRIDEiP0AFH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74417; AAC74417; b1335.
BAA14928; BAA14928; BAA14928.
GeneIDi945853.
KEGGiecj:JW1329.
eco:b1335.
PATRICi32117946. VBIEscCol129921_1393.

Organism-specific databases

EchoBASEiEB0662.
EcoGeneiEG10668. ogt.

Phylogenomic databases

eggNOGiENOG4105K85. Bacteria.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiP0AFH0.
KOiK00567.
OMAiEDKIATP.
PhylomeDBiP0AFH0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10668-MONOMER.
ECOL316407:JW1329-MONOMER.
MetaCyc:EG10668-MONOMER.

Miscellaneous databases

PROiP0AFH0.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGT_ECOLI
AccessioniPrimary (citable) accession number: P0AFH0
Secondary accession number(s): P09168, P78284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.