P0AFH0 (OGT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	ogt
Ordered Locus Names:	b1335, JW1329

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139365
Sites
	Active site	139	1	Nucleophile; methyl group acceptor Probable
Experimental info
	Mutagenesis	102	1	C → G: Little effect on the activity. Ref.5
	Mutagenesis	139	1	C → G: Eliminates the activity. Ref.5
	Sequence conflict	37	1	R → A in CAA68548. Ref.1
	Sequence conflict	68	1	E → D in CAA68548. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AFH0 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 39EFBF1539F67D26	FASTA	171	19,179
	10 20 30 40 50 60 MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG 70 80 90 100 110 120 GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA 130 140 150 160 170 ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli." Potter P.M., Wilkinson M.C., Fitton J., Carr F.J., Brennand J., Cooper D.P., Margison G.P. Nucleic Acids Res. 15:9177-9193(1987) [PubMed: 2825131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein." Harris L.C., Potter P.M., Margison G.P. Biochem. Biophys. Res. Commun. 187:425-431(1992) [PubMed: 1520330] [Abstract] Cited for: MUTAGENESIS OF CYSTEINE RESIDUES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00495 Genomic DNA. Translation: CAA68548.1. U00096 Genomic DNA. Translation: AAC74417.1. AP009048 Genomic DNA. Translation: BAA14928.1.
PIR	XUECAD. B64883.
RefSeq	NP_415851.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AFH0.
SMR	P0AFH0. Positions 2-170.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48073N.
IntAct	P0AFH0. 6 interactions.
MINT	MINT-1243838.
Proteomic databases
PRIDE	P0AFH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003089; EBESCP00000003089; EBESCG00000002531. EBESCT00000017511; EBESCP00000016802; EBESCG00000016567.
GeneID	945853.
GenomeReviews	Gene locus JW1329 in contig AP009048_GR. Gene locus b1335 in contig U00096_GR.
KEGG	ecj:JW1329. eco:b1335.
PATRIC	32117946. VBIEscCol129921_1393.
Organism-specific databases
EchoBASE	EB0662.
EcoGene	EG10668. ogt.
Phylogenomic databases
eggNOG	COG0350.
GeneTree	EBGT00050000009234.
HOGENOM	HBG752398.
OMA	NLRAVEW.
PhylomeDB	P0AFH0.
ProtClustDB	PRK10286.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10668-MONOMER. MetaCyc:EG10668-MONOMER.
Gene expression databases
Genevestigator	P0AFH0.
Family and domain databases
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR008332. MethylG_MeTrfase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K00567.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. Ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OGT_ECOLI

Accession

Primary (citable) accession number: P0AFH0
Secondary accession number(s): P09168, P78284

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program