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P0AFG9

- ODP1_ECO57

UniProt

P0AFG9 - ODP1_ECO57

Protein

Pyruvate dehydrogenase E1 component

Gene

aceE

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.By similarity

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Magnesium.By similarity
    Thiamine pyrophosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi231 – 2311MagnesiumBy similarity
    Metal bindingi261 – 2611MagnesiumBy similarity
    Metal bindingi263 – 2631Magnesium; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding, Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-115-MONOMER.
    ECOO157:ACEE-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component (EC:1.2.4.1)
    Short name:
    PDH E1 component
    Gene namesi
    Name:aceE
    Ordered Locus Names:Z0124, ECs0118
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 887886Pyruvate dehydrogenase E1 componentPRO_0000162244Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei716 – 7161N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP0AFG9.

    Interactioni

    Subunit structurei

    Homodimer. Part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).By similarity

    Protein-protein interaction databases

    IntActiP0AFG9. 3 interactions.
    STRINGi155864.Z0124.

    Structurei

    Secondary structure

    1
    887
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi66 – 683
    Helixi76 – 9924
    Helixi109 – 12416
    Beta strandi131 – 1333
    Beta strandi137 – 1393
    Helixi142 – 1443
    Helixi145 – 15410
    Helixi160 – 1634
    Turni181 – 1833
    Turni185 – 1873
    Helixi197 – 21418
    Beta strandi225 – 2306
    Helixi233 – 2353
    Helixi237 – 2404
    Helixi243 – 2486
    Beta strandi254 – 2607
    Beta strandi265 – 2695
    Beta strandi271 – 2733
    Helixi275 – 28511
    Beta strandi289 – 2935
    Helixi297 – 2993
    Helixi300 – 3056
    Helixi310 – 3178
    Helixi320 – 3267
    Helixi331 – 3377
    Helixi339 – 3413
    Helixi343 – 3464
    Turni347 – 3493
    Beta strandi350 – 3523
    Helixi354 – 3574
    Helixi363 – 3653
    Helixi367 – 37913
    Beta strandi385 – 3906
    Turni393 – 3964
    Helixi416 – 4249
    Helixi431 – 4344
    Helixi447 – 45711
    Turni458 – 4603
    Helixi479 – 4824
    Helixi483 – 4864
    Helixi495 – 50612
    Turni510 – 5156
    Beta strandi516 – 5227
    Helixi525 – 5273
    Helixi530 – 5367
    Beta strandi565 – 5673
    Helixi572 – 58312
    Helixi585 – 5884
    Beta strandi594 – 6007
    Helixi601 – 6033
    Helixi605 – 61713
    Beta strandi623 – 6286
    Turni631 – 6333
    Turni635 – 6373
    Turni639 – 6413
    Helixi646 – 6505
    Beta strandi656 – 6594
    Helixi664 – 67916
    Beta strandi687 – 6915
    Helixi707 – 7126
    Beta strandi714 – 7207
    Beta strandi726 – 7316
    Helixi733 – 7353
    Helixi736 – 75015
    Beta strandi753 – 7608
    Helixi762 – 77817
    Helixi788 – 7925
    Beta strandi798 – 8014
    Helixi807 – 8104
    Helixi811 – 8155
    Beta strandi817 – 8193
    Beta strandi821 – 8244
    Helixi835 – 8417
    Helixi846 – 85914
    Helixi865 – 87410
    Turni884 – 8863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LPLX-ray2.10A/B2-887[»]
    3LQ2X-ray1.96A/B2-887[»]
    3LQ4X-ray1.98A/B2-887[»]
    ProteinModelPortaliP0AFG9.
    SMRiP0AFG9. Positions 57-887.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AFG9.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG2609.
    HOGENOMiHOG000115215.
    KOiK00163.
    OMAiKGIYKLD.
    OrthoDBiEOG6BW4TW.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR004660. 2-oxoA_DH_E1.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005474. Transketolase_N.
    [Graphical view]
    PfamiPF00456. Transketolase_N. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000156. Pyruvate_dh_E1. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    SSF52922. SSF52922. 1 hit.
    TIGRFAMsiTIGR00759. aceE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AFG9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSERFPNDVD PIETRDWLQA IESVIREEGV ERAQYLIDQL LAEARKGGVN    50
    VAAGTGISNY INTIPVEEQP EYPGNLELER RIRSAIRWNA IMTVLRASKK 100
    DLELGGHMAS FQSSATIYDV CFNHFFRARN EQDGGDLVYF QGHISPGVYA 150
    RAFLEGRLTQ EQLDNFRQEV HGNGLSSYPH PKLMPEFWQF PTVSMGLGPI 200
    GAIYQAKFLK YLEHRGLKDT SKQTVYAFLG DGEMDEPESK GAITIATREK 250
    LDNLVFVINC NLQRLDGPVT GNGKIINELE GIFEGAGWNV IKVMWGSRWD 300
    ELLRKDTSGK LIQLMNETVD GDYQTFKSKD GAYVREHFFG KYPETAALVA 350
    DWTDEQIWAL NRGGHDPKKI YAAFKKAQET KGKATVILAH TIKGYGMGDA 400
    AEGKNIAHQV KKMNMDGVRH IRDRFNVPVS DADIEKLPYI TFPEGSEEHT 450
    YLHAQRQKLH GYLPSRQPNF TEKLELPSLQ DFGALLEEQS KEISTTIAFV 500
    RALNVMLKNK SIKDRLVPII ADEARTFGME GLFRQIGIYS PNGQQYTPQD 550
    REQVAYYKED EKGQILQEGI NELGAGCSWL AAATSYSTNN LPMIPFYIYY 600
    SMFGFQRIGD LCWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS 650
    LTIPNCISYD PAYAYEVAVI MHDGLERMYG EKQENVYYYI TTLNENYHMP 700
    AMPEGAEEGI RKGIYKLETI EGSKGKVQLL GSGSILRHVR EAAEILAKDY 750
    GVGSDVYSVT SFTELARDGQ DCERWNMLHP LETPRVPYIA QVMNDAPAVA 800
    STDYMKLFAE QVRTYVPADD YRVLGTDGFG RSDSRENLRH HFEVDASYVV 850
    VAALGELAKR GEIDKKVVAD AIAKFNIDAD KVNPRLA 887
    Length:887
    Mass (Da):99,668
    Last modified:January 23, 2007 - v2
    Checksum:i7FB3811DE11BDD02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54418.1.
    BA000007 Genomic DNA. Translation: BAB33541.1.
    PIRiF85494.
    F90643.
    RefSeqiNP_285810.1. NC_002655.2.
    NP_308145.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG54418; AAG54418; Z0124.
    BAB33541; BAB33541; BAB33541.
    GeneIDi913657.
    956809.
    KEGGiece:Z0124.
    ecs:ECs0118.
    PATRICi18349174. VBIEscCol44059_0117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54418.1 .
    BA000007 Genomic DNA. Translation: BAB33541.1 .
    PIRi F85494.
    F90643.
    RefSeqi NP_285810.1. NC_002655.2.
    NP_308145.1. NC_002695.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LPL X-ray 2.10 A/B 2-887 [» ]
    3LQ2 X-ray 1.96 A/B 2-887 [» ]
    3LQ4 X-ray 1.98 A/B 2-887 [» ]
    ProteinModelPortali P0AFG9.
    SMRi P0AFG9. Positions 57-887.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0AFG9. 3 interactions.
    STRINGi 155864.Z0124.

    Proteomic databases

    PRIDEi P0AFG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG54418 ; AAG54418 ; Z0124 .
    BAB33541 ; BAB33541 ; BAB33541 .
    GeneIDi 913657.
    956809.
    KEGGi ece:Z0124.
    ecs:ECs0118.
    PATRICi 18349174. VBIEscCol44059_0117.

    Phylogenomic databases

    eggNOGi COG2609.
    HOGENOMi HOG000115215.
    KOi K00163.
    OMAi KGIYKLD.
    OrthoDBi EOG6BW4TW.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-115-MONOMER.
    ECOO157:ACEE-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AFG9.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR004660. 2-oxoA_DH_E1.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005474. Transketolase_N.
    [Graphical view ]
    Pfami PF00456. Transketolase_N. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000156. Pyruvate_dh_E1. 1 hit.
    SUPFAMi SSF52518. SSF52518. 2 hits.
    SSF52922. SSF52922. 1 hit.
    TIGRFAMsi TIGR00759. aceE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiODP1_ECO57
    AccessioniPrimary (citable) accession number: P0AFG9
    Secondary accession number(s): P06958, P78049, Q53382
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3