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P0AFF4 (NUPG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nucleoside permease nupG
Alternative name(s):
Nucleoside-transport system protein nupG
Gene names
Name:nupG
Ordered Locus Names:b2964, JW2932
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transports nucleosides with a high affinity. Driven by a proton motive force.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

To E.coli xapB and yegT.

Sequence caution

The sequence AAA69132.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to plasma membrane

Inferred from direct assay. Source: EcoliWiki

   Molecular functionuridine transmembrane transporter activity

Inferred from direct assay. Source: EcoliWiki

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Nucleoside permease nupG
PRO_0000058005

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2921Helical; Potential
Topological domain30 – 3910Periplasmic Potential
Transmembrane40 – 6021Helical; Potential
Topological domain61 – 699Cytoplasmic Potential
Transmembrane70 – 9021Helical; Potential
Topological domain91 – 955Periplasmic Potential
Transmembrane96 – 11621Helical; Potential
Topological domain117 – 13620Cytoplasmic Potential
Transmembrane137 – 15721Helical; Potential
Topological domain158 – 1614Periplasmic Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 20927Cytoplasmic Potential
Transmembrane210 – 23021Helical; Potential
Topological domain231 – 25424Periplasmic Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 2827Cytoplasmic Potential
Transmembrane283 – 30321Helical; Potential
Topological domain3041Periplasmic Potential
Transmembrane305 – 32521Helical; Potential
Topological domain326 – 34823Cytoplasmic Potential
Transmembrane349 – 36921Helical; Potential
Topological domain370 – 38112Periplasmic Potential
Transmembrane382 – 40221Helical; Potential
Topological domain403 – 41816Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P0AFF4 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 01337ECEFC369266

FASTA41846,389
        10         20         30         40         50         60 
MNLKLQLKIL SFLQFCLWGS WLTTLGSYMF VTLKFDGASI GAVYSSLGIA AVFMPALLGI 

        70         80         90        100        110        120 
VADKWLSAKW VYAICHTIGA ITLFMAAQVT TPEAMFLVIL INSFAYMPTL GLINTISYYR 

       130        140        150        160        170        180 
LQNAGMDIVT DFPPIRIWGT IGFIMAMWVV SLSGFELSHM QLYIGAALSA ILVLFTLTLP 

       190        200        210        220        230        240 
HIPVAKQQAN QSWTTLLGLD AFALFKNKRM AIFFIFSMLL GAELQITNMF GNTFLHSFDK 

       250        260        270        280        290        300 
DPMFASSFIV QHASIIMSIS QISETLFILT IPFFLSRYGI KNVMMISIVA WILRFALFAY 

       310        320        330        340        350        360 
GDPTPFGTVL LVLSMIVYGC AFDFFNISGS VFVEKEVSPA IRASAQGMFL MMTNGFGCIL 

       370        380        390        400        410 
GGIVSGKVVE MYTQNGITDW QTVWLIFAGY SVVLAFAFMA MFKYKHVRVP TGTQTVSH

« Hide

References

« Hide 'large scale' references
[1]"Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system."
Westh Hansen S.E., Jensen N., Munch-Petersen A.
Eur. J. Biochem. 168:385-391(1987) [PubMed: 3311747] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X06174 Genomic DNA. Translation: CAA29541.1.
U28377 Genomic DNA. Translation: AAA69132.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76001.2.
AP009048 Genomic DNA. Translation: BAE77027.1.
RefSeqNP_417439.4. NC_000913.2.

3D structure databases

ProteinModelPortalP0AFF4.
ModBaseSearch...

Protein family/group databases

TCDB2.A.1.10.1. major facilitator superfamily (MFS).

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002361; EBESCP00000002361; EBESCG00000001928.
EBESCT00000002362; EBESCP00000002362; EBESCG00000001928.
EBESCT00000002363; EBESCP00000002363; EBESCG00000001928.
EBESCT00000002364; EBESCP00000002364; EBESCG00000001928.
EBESCT00000016941; EBESCP00000016232; EBESCG00000016000.
GeneID946282.
GenomeReviewsGene locus JW2932 in contig AP009048_GR.
Gene locus b2964 in contig U00096_GR.
KEGGecj:JW2932.
eco:b2964.
PATRIC32121344. VBIEscCol129921_3060.

Organism-specific databases

EchoBASEEB0658.
EcoGeneEG10664. nupG.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeEBGT00050000008944.
HOGENOMHBG347023.
OMAFIVEHAS.
PhylomeDBP0AFF4.
ProtClustDBCLSK880543.

Enzyme and pathway databases

BioCycEcoCyc:NUPG-MONOMER.

Gene expression databases

GenevestigatorP0AFF4.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004740. Nuc_H_symport.
[Graphical view]
KOK03289.
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMsTIGR00889. 2A0110. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUPG_ECOLI
AccessionPrimary (citable) accession number: P0AFF4
Secondary accession number(s): P09452, P76653, Q2M9M9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents