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Protein

Nucleoside permease NupG

Gene

nupG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad-specificity transporter of purine and pyrimidine nucleosides. Driven by a proton motive force. Can transport uridine, adenosine, inosine, guanosine, thymidine and cytidine. Can also transport xanthosine, but with a very low affinity.5 Publications

Enzyme regulationi

Inhibited by the protonophore uncouplers 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by valinomycin.1 Publication

Kineticsi

  1. KM=23.6 µM for uridine1 Publication
  2. KM=20.6 µM for adenosine1 Publication
  1. Vmax=67.2 nmol/min/mg enzyme with uridine as substrate1 Publication
  2. Vmax=56.8 nmol/min/mg enzyme with adenosine as substrate1 Publication

GO - Molecular functioni

  • cytidine transmembrane transporter activity Source: EcoliWiki
  • nucleoside:proton symporter activity Source: UniProtKB-HAMAP
  • pyrimidine nucleoside transmembrane transporter activity Source: EcoliWiki
  • uridine transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • adenosine transport Source: EcoliWiki
  • purine nucleoside transmembrane transport Source: EcoliWiki
  • pyrimidine nucleoside transport Source: EcoliWiki
  • uridine transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:NUPG-MONOMER.
ECOL316407:JW2932-MONOMER.
MetaCyc:NUPG-MONOMER.

Protein family/group databases

TCDBi2.A.1.10.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside permease NupGUniRule annotationCurated
Alternative name(s):
Nucleoside-transport system protein NupGCurated
Gene namesi
Name:nupG1 PublicationUniRule annotation
Ordered Locus Names:b2964, JW2932
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10664. nupG.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicCurated8
Transmembranei9 – 29HelicalUniRule annotationAdd BLAST21
Topological domaini30 – 39PeriplasmicCurated10
Transmembranei40 – 60HelicalUniRule annotationAdd BLAST21
Topological domaini61 – 69CytoplasmicCurated9
Transmembranei70 – 90HelicalUniRule annotationAdd BLAST21
Topological domaini91 – 95PeriplasmicCurated5
Transmembranei96 – 116HelicalUniRule annotationAdd BLAST21
Topological domaini117 – 136CytoplasmicCuratedAdd BLAST20
Transmembranei137 – 157HelicalUniRule annotationAdd BLAST21
Topological domaini158 – 161PeriplasmicCurated4
Transmembranei162 – 182HelicalUniRule annotationAdd BLAST21
Topological domaini183 – 209CytoplasmicCuratedAdd BLAST27
Transmembranei210 – 230HelicalUniRule annotationAdd BLAST21
Topological domaini231 – 254PeriplasmicCuratedAdd BLAST24
Transmembranei255 – 275HelicalUniRule annotationAdd BLAST21
Topological domaini276 – 282CytoplasmicCurated7
Transmembranei283 – 303HelicalUniRule annotationAdd BLAST21
Topological domaini304PeriplasmicCurated1
Transmembranei305 – 325HelicalUniRule annotationAdd BLAST21
Topological domaini326 – 348CytoplasmicCuratedAdd BLAST23
Transmembranei349 – 369HelicalUniRule annotationAdd BLAST21
Topological domaini370 – 381PeriplasmicCuratedAdd BLAST12
Transmembranei382 – 402HelicalUniRule annotationAdd BLAST21
Topological domaini403 – 418Cytoplasmic1 PublicationAdd BLAST16

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580051 – 418Nucleoside permease NupGAdd BLAST418

Proteomic databases

PaxDbiP0AFF4.
PRIDEiP0AFF4.

Expressioni

Inductioni

Transcriptionally regulated by CytR and DeoR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262362. 111 interactors.
STRINGi511145.b2964.

Structurei

3D structure databases

ProteinModelPortaliP0AFF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily. Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family. [View classification]UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAA. Bacteria.
ENOG410ZVFU. LUCA.
HOGENOMiHOG000026502.
InParanoidiP0AFF4.
KOiK03289.
OMAiQGLFMMM.
PhylomeDBiP0AFF4.

Family and domain databases

HAMAPiMF_02049. NupG. 1 hit.
InterProiIPR020846. MFS_dom.
IPR004740. Nuc_H_symport.
IPR033667. NupG.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00889. 2A0110. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKLQLKIL SFLQFCLWGS WLTTLGSYMF VTLKFDGASI GAVYSSLGIA
60 70 80 90 100
AVFMPALLGI VADKWLSAKW VYAICHTIGA ITLFMAAQVT TPEAMFLVIL
110 120 130 140 150
INSFAYMPTL GLINTISYYR LQNAGMDIVT DFPPIRIWGT IGFIMAMWVV
160 170 180 190 200
SLSGFELSHM QLYIGAALSA ILVLFTLTLP HIPVAKQQAN QSWTTLLGLD
210 220 230 240 250
AFALFKNKRM AIFFIFSMLL GAELQITNMF GNTFLHSFDK DPMFASSFIV
260 270 280 290 300
QHASIIMSIS QISETLFILT IPFFLSRYGI KNVMMISIVA WILRFALFAY
310 320 330 340 350
GDPTPFGTVL LVLSMIVYGC AFDFFNISGS VFVEKEVSPA IRASAQGMFL
360 370 380 390 400
MMTNGFGCIL GGIVSGKVVE MYTQNGITDW QTVWLIFAGY SVVLAFAFMA
410
MFKYKHVRVP TGTQTVSH
Length:418
Mass (Da):46,389
Last modified:December 20, 2005 - v1
Checksum:i01337ECEFC369266
GO

Sequence cautioni

The sequence AAA69132 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3L → S AA sequence (PubMed:15513740).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06174 Genomic DNA. Translation: CAA29541.1.
U28377 Genomic DNA. Translation: AAA69132.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76001.2.
AP009048 Genomic DNA. Translation: BAE77027.1.
RefSeqiNP_417439.4. NC_000913.3.
WP_001049791.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76001; AAC76001; b2964.
BAE77027; BAE77027; BAE77027.
GeneIDi946282.
KEGGiecj:JW2932.
eco:b2964.
PATRICi32121344. VBIEscCol129921_3060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06174 Genomic DNA. Translation: CAA29541.1.
U28377 Genomic DNA. Translation: AAA69132.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76001.2.
AP009048 Genomic DNA. Translation: BAE77027.1.
RefSeqiNP_417439.4. NC_000913.3.
WP_001049791.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262362. 111 interactors.
STRINGi511145.b2964.

Protein family/group databases

TCDBi2.A.1.10.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0AFF4.
PRIDEiP0AFF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76001; AAC76001; b2964.
BAE77027; BAE77027; BAE77027.
GeneIDi946282.
KEGGiecj:JW2932.
eco:b2964.
PATRICi32121344. VBIEscCol129921_3060.

Organism-specific databases

EchoBASEiEB0658.
EcoGeneiEG10664. nupG.

Phylogenomic databases

eggNOGiENOG4105EAA. Bacteria.
ENOG410ZVFU. LUCA.
HOGENOMiHOG000026502.
InParanoidiP0AFF4.
KOiK03289.
OMAiQGLFMMM.
PhylomeDBiP0AFF4.

Enzyme and pathway databases

BioCyciEcoCyc:NUPG-MONOMER.
ECOL316407:JW2932-MONOMER.
MetaCyc:NUPG-MONOMER.

Miscellaneous databases

PROiP0AFF4.

Family and domain databases

HAMAPiMF_02049. NupG. 1 hit.
InterProiIPR020846. MFS_dom.
IPR004740. Nuc_H_symport.
IPR033667. NupG.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00889. 2A0110. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUPG_ECOLI
AccessioniPrimary (citable) accession number: P0AFF4
Secondary accession number(s): P09452, P76653, Q2M9M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Binding of nucleosides to NupG requires the presence of hydroxyl groups at each of the C-3' and C-5' positions of ribose.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.