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Protein

Nucleoside permease NupC

Gene

nupC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force. Transports cytidine, uridine, thymidine, adenosine and inosine. Can also transport xanthosine, but with a very low affinity.3 Publications

GO - Molecular functioni

  • cytidine transmembrane transporter activity Source: EcoliWiki
  • nucleoside:proton symporter activity Source: EcoCyc
  • uridine transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • nucleoside transmembrane transport Source: EcoliWiki
  • nucleoside transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:NUPC-MONOMER.
ECOL316407:JW2389-MONOMER.
MetaCyc:NUPC-MONOMER.

Protein family/group databases

TCDBi2.A.41.1.1. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside permease NupCCurated
Alternative name(s):
Nucleoside-transport system protein NupCCurated
Gene namesi
Name:nupC1 Publication
Synonyms:cru
Ordered Locus Names:b2393, JW2389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11971. nupC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicCurated
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 3612PeriplasmicCuratedAdd
BLAST
Transmembranei37 – 5721HelicalSequence analysisAdd
BLAST
Topological domaini58 – 8629CytoplasmicCuratedAdd
BLAST
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST
Topological domaini108 – 16861PeriplasmicCuratedAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Topological domaini190 – 1923CytoplasmicCurated
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Topological domaini214 – 25037PeriplasmicCuratedAdd
BLAST
Transmembranei251 – 27121HelicalSequence analysisAdd
BLAST
Topological domaini272 – 28110CytoplasmicCurated
Transmembranei282 – 30221HelicalSequence analysisAdd
BLAST
Topological domaini303 – 34139PeriplasmicCuratedAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 37816CytoplasmicCuratedAdd
BLAST
Transmembranei379 – 39921HelicalSequence analysisAdd
BLAST
Topological domaini400 – 4001Periplasmic1 Publication

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Nucleoside permease NupCPRO_0000070454Add
BLAST

Proteomic databases

PaxDbiP0AFF2.

Interactioni

Protein-protein interaction databases

BioGridi4259184. 8 interactions.
STRINGi511145.b2393.

Structurei

3D structure databases

ProteinModelPortaliP0AFF2.
SMRiP0AFF2. Positions 10-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D9N. Bacteria.
COG1972. LUCA.
HOGENOMiHOG000267657.
InParanoidiP0AFF2.
KOiK11535.
OMAiNEFVAMN.
PhylomeDBiP0AFF2.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRVLHFVLA LAVVAILALL VSSDRKKIRI RYVIQLLVIE VLLAWFFLNS
60 70 80 90 100
DVGLGFVKGF SEMFEKLLGF ANEGTNFVFG SMNDQGLAFF FLKVLCPIVF
110 120 130 140 150
ISALIGILQH IRVLPVIIRA IGFLLSKVNG MGKLESFNAV SSLILGQSEN
160 170 180 190 200
FIAYKDILGK ISRNRMYTMA ATAMSTVSMS IVGAYMTMLE PKYVVAALVL
210 220 230 240 250
NMFSTFIVLS LINPYRVDAS EENIQMSNLH EGQSFFEMLG EYILAGFKVA
260 270 280 290 300
IIVAAMLIGF IALIAALNAL FATVTGWFGY SISFQGILGY IFYPIAWVMG
310 320 330 340 350
VPSSEALQVG SIMATKLVSN EFVAMMDLQK IASTLSPRAE GIISVFLVSF
360 370 380 390 400
ANFSSIGIIA GAVKGLNEEQ GNVVSRFGLK LVYGSTLVSV LSASIAALVL
Length:400
Mass (Da):43,476
Last modified:December 20, 2005 - v1
Checksum:i1EA1DE138C4351CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821Missing in CAA52821 (PubMed:8022285).Curated
Sequence conflicti88 – 881A → AE in CAA52821 (PubMed:8022285).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74825 Genomic DNA. Translation: CAA52821.1.
U00096 Genomic DNA. Translation: AAC75452.1.
AP009048 Genomic DNA. Translation: BAA16263.1.
PIRiF65013.
RefSeqiNP_416894.1. NC_000913.3.
WP_000376337.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75452; AAC75452; b2393.
BAA16263; BAA16263; BAA16263.
GeneIDi946895.
KEGGiecj:JW2389.
eco:b2393.
PATRICi32120163. VBIEscCol129921_2490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74825 Genomic DNA. Translation: CAA52821.1.
U00096 Genomic DNA. Translation: AAC75452.1.
AP009048 Genomic DNA. Translation: BAA16263.1.
PIRiF65013.
RefSeqiNP_416894.1. NC_000913.3.
WP_000376337.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFF2.
SMRiP0AFF2. Positions 10-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259184. 8 interactions.
STRINGi511145.b2393.

Protein family/group databases

TCDBi2.A.41.1.1. the concentrative nucleoside transporter (cnt) family.

Proteomic databases

PaxDbiP0AFF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75452; AAC75452; b2393.
BAA16263; BAA16263; BAA16263.
GeneIDi946895.
KEGGiecj:JW2389.
eco:b2393.
PATRICi32120163. VBIEscCol129921_2490.

Organism-specific databases

EchoBASEiEB1914.
EcoGeneiEG11971. nupC.

Phylogenomic databases

eggNOGiENOG4105D9N. Bacteria.
COG1972. LUCA.
HOGENOMiHOG000267657.
InParanoidiP0AFF2.
KOiK11535.
OMAiNEFVAMN.
PhylomeDBiP0AFF2.

Enzyme and pathway databases

BioCyciEcoCyc:NUPC-MONOMER.
ECOL316407:JW2389-MONOMER.
MetaCyc:NUPC-MONOMER.

Miscellaneous databases

PROiP0AFF2.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUPC_ECOLI
AccessioniPrimary (citable) accession number: P0AFF2
Secondary accession number(s): P33031, P77236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Binding of nucleosides to NupC requires the presence of hydroxyl groups at the C-3' position of ribose.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.