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Protein

NADH-quinone oxidoreductase subunit N

Gene

nuoN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • ATP synthesis coupled electron transport Source: InterPro
  • electron transport coupled proton transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUON-MONOMER.
ECOL316407:JW2271-MONOMER.
MetaCyc:NUON-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit NUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit NUniRule annotation
NDH-1 subunit NUniRule annotation
NUO14
Gene namesi
Name:nuoNUniRule annotation
Ordered Locus Names:b2276, JW2271
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12093. nuoN.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77PeriplasmicSequence analysis
Transmembranei8 – 2821HelicalUniRule annotationAdd
BLAST
Topological domaini29 – 346CytoplasmicSequence analysis
Transmembranei35 – 5521HelicalUniRule annotationAdd
BLAST
Topological domaini56 – 7015PeriplasmicSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalUniRule annotationAdd
BLAST
Topological domaini92 – 10413CytoplasmicSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalUniRule annotationAdd
BLAST
Topological domaini126 – 1261PeriplasmicSequence analysis
Transmembranei127 – 14721HelicalUniRule annotationAdd
BLAST
Topological domaini148 – 15811CytoplasmicSequence analysisAdd
BLAST
Transmembranei159 – 17921HelicalUniRule annotationAdd
BLAST
Topological domaini180 – 20223PeriplasmicSequence analysisAdd
BLAST
Transmembranei203 – 22321HelicalUniRule annotationAdd
BLAST
Topological domaini224 – 23411CytoplasmicSequence analysisAdd
BLAST
Transmembranei235 – 25521HelicalUniRule annotationAdd
BLAST
Topological domaini256 – 27015PeriplasmicSequence analysisAdd
BLAST
Transmembranei271 – 29121HelicalUniRule annotationAdd
BLAST
Topological domaini292 – 2965CytoplasmicSequence analysis
Transmembranei297 – 31721HelicalUniRule annotationAdd
BLAST
Topological domaini318 – 3258PeriplasmicSequence analysis
Transmembranei326 – 34621HelicalUniRule annotationAdd
BLAST
Topological domaini347 – 37226CytoplasmicSequence analysisAdd
BLAST
Transmembranei373 – 39321HelicalUniRule annotationAdd
BLAST
Topological domaini394 – 40714PeriplasmicSequence analysisAdd
BLAST
Transmembranei408 – 43023HelicalUniRule annotationAdd
BLAST
Topological domaini431 – 45424CytoplasmicSequence analysisAdd
BLAST
Transmembranei455 – 47521HelicalUniRule annotationAdd
BLAST
Topological domaini476 – 48510PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells gene grow very poorly on M65 minimal medium containing acetate but normally on rich medium (LB).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → H: Shows 20% of the wild-type rate of deamino-NADH oxidase. 1 Publication
Mutagenesisi158 – 1581K → C: Shows 50% of the wild-type rate of deamino-NADH oxidase. Inhibited by 30-50% upon addition of 0.25 mM of decylubiquinone. 1 Publication
Mutagenesisi217 – 2171K → C: Loss of activity. 1 Publication
Mutagenesisi217 – 2171K → R: Shows 40% of the wild-type rate of deamino-NADH oxidase. 1 Publication
Mutagenesisi224 – 2241H → K: Shows 40% of the wild-type rate of deamino-NADH oxidase. Inhibited by 20-30% upon addition of 0.25 mM of decylubiquinone. 1 Publication
Mutagenesisi247 – 2471K → C: Shows 7% of the wild-type rate of deamino-NADH oxidase. 1 Publication
Mutagenesisi391 – 3911G → S: Shows 90% of the wild-type rate of deamino-NADH oxidase. 1 Publication
Mutagenesisi395 – 3951K → C: Shows 5% of the wild-type rate of deamino-NADH oxidase. 1 Publication
Mutagenesisi395 – 3951K → R: Shows 30% of the wild-type rate of deamino-NADH oxidase. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485NADH-quinone oxidoreductase subunit NPRO_0000117688Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFF0.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260885. 25 interactions.
DIPiDIP-59256N.
STRINGi511145.b2276.

Structurei

3D structure databases

ProteinModelPortaliP0AFF0.
SMRiP0AFF0. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNR. Bacteria.
COG1007. LUCA.
HOGENOMiHOG000100796.
InParanoidiP0AFF0.
KOiK00343.
OMAiMLAIAWK.
OrthoDBiEOG64JFNZ.
PhylomeDBiP0AFF0.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITPQNLIA LLPLLIVGLT VVVVMLSIAW RRNHFLNATL SVIGLNAALV
60 70 80 90 100
SLWFVGQAGA MDVTPLMRVD GFAMLYTGLV LLASLATCTF AYPWLEGYND
110 120 130 140 150
NKDEFYLLVL IAALGGILLA NANHLASLFL GIELISLPLF GLVGYAFRQK
160 170 180 190 200
RSLEASIKYT ILSAAASSFL LFGMALVYAQ SGDLSFVALG KNLGDGMLNE
210 220 230 240 250
PLLLAGFGLM IVGLGFKLSL VPFHLWTPDV YQGAPAPVST FLATASKIAI
260 270 280 290 300
FGVVMRLFLY APVGDSEAIR VVLAIIAFAS IIFGNLMALS QTNIKRLLGY
310 320 330 340 350
SSISHLGYLL VALIALQTGE MSMEAVGVYL AGYLFSSLGA FGVVSLMSSP
360 370 380 390 400
YRGPDADSLF SYRGLFWHRP ILAAVMTVMM LSLAGIPMTL GFIGKFYVLA
410 420 430 440 450
VGVQAHLWWL VGAVVVGSAI GLYYYLRVAV SLYLHAPEQP GRDAPSNWQY
460 470 480
SAGGIVVLIS ALLVLVLGVW PQPLISIVRL AMPLM
Length:485
Mass (Da):52,044
Last modified:September 5, 2006 - v2
Checksum:iA15F4875B7D19D09
GO

Sequence cautioni

The sequence BAA16103.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA48373.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1264NHLA → TIWR in CAA48373 (PubMed:7690854).Curated
Sequence conflicti200 – 2012EP → DA in CAA48373 (PubMed:7690854).Curated
Sequence conflicti268 – 27710AIRVVLAIIA → QVRRGAGDYR in CAA48373 (PubMed:7690854).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48373.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75336.2.
AP009048 Genomic DNA. Translation: BAA16103.1. Different initiation.
PIRiB64999.
S38323.
RefSeqiNP_416779.2. NC_000913.3.
WP_000156701.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75336; AAC75336; b2276.
BAA16103; BAA16103; BAA16103.
GeneIDi945136.
KEGGiecj:JW2271.
eco:b2276.
PATRICi32119919. VBIEscCol129921_2369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48373.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75336.2.
AP009048 Genomic DNA. Translation: BAA16103.1. Different initiation.
PIRiB64999.
S38323.
RefSeqiNP_416779.2. NC_000913.3.
WP_000156701.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFF0.
SMRiP0AFF0. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260885. 25 interactions.
DIPiDIP-59256N.
STRINGi511145.b2276.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75336; AAC75336; b2276.
BAA16103; BAA16103; BAA16103.
GeneIDi945136.
KEGGiecj:JW2271.
eco:b2276.
PATRICi32119919. VBIEscCol129921_2369.

Organism-specific databases

EchoBASEiEB2017.
EcoGeneiEG12093. nuoN.

Phylogenomic databases

eggNOGiENOG4105CNR. Bacteria.
COG1007. LUCA.
HOGENOMiHOG000100796.
InParanoidiP0AFF0.
KOiK00343.
OMAiMLAIAWK.
OrthoDBiEOG64JFNZ.
PhylomeDBiP0AFF0.

Enzyme and pathway databases

BioCyciEcoCyc:NUON-MONOMER.
ECOL316407:JW2271-MONOMER.
MetaCyc:NUON-MONOMER.

Miscellaneous databases

PROiP0AFF0.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I."
    Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H.
    J. Mol. Biol. 233:109-122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / AN387.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone."
    Amarneh B., Vik S.B.
    Biochemistry 42:4800-4808(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF START CODON, MUTAGENESIS OF MET-1; LYS-158; LYS-217; HIS-224; LYS-247; GLY-391 AND LYS-395, DISRUPTION PHENOTYPE.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNUON_ECOLI
AccessioniPrimary (citable) accession number: P0AFF0
Secondary accession number(s): P33608, P78281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 5, 2006
Last modified: January 20, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A construct which produces a protein lacking the first amino acids has only 5% of the wild-type rate of deamino-NADH oxidase.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.