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Protein

NADH-quinone oxidoreductase subunit K

Gene

nuoK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy-5-methyl-6-decyl-1,4-benzoquinone) as substrates.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTransport
LigandNAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOK-MONOMER
MetaCyc:NUOK-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit KUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit KUniRule annotation
NDH-1 subunit KUniRule annotation
NUO11
Gene namesi
Name:nuoKUniRule annotation
Ordered Locus Names:b2279, JW2274
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12091 nuoK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3PeriplasmicSequence analysis3
Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21
Topological domaini25 – 27CytoplasmicSequence analysis3
Transmembranei28 – 48HelicalUniRule annotationAdd BLAST21
Topological domaini49 – 59PeriplasmicSequence analysisAdd BLAST11
Transmembranei60 – 80HelicalUniRule annotationAdd BLAST21
Topological domaini81 – 100CytoplasmicSequence analysisAdd BLAST20

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25 – 26RR → AA: Retains 14% dNADH oxidase and dNADH-DB reductase activity. 1 Publication2
Mutagenesisi36E → A: Retains 2% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1
Mutagenesisi36E → Q: Retains 2% dNADH oxidase and dNADH-DB reductase activity, neither a membrane potential nor a proton gradient can be formed by isolated membranes. 1 Publication1
Mutagenesisi72E → A: Retains 43% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1
Mutagenesisi72E → Q: Retains 22% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185241 – 100NADH-quinone oxidoreductase subunit KAdd BLAST100

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFE4
PRIDEiP0AFE4

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260508, 69 interactors
DIPiDIP-51165N
IntActiP0AFE4, 2 interactors
STRINGi316385.ECDH10B_2441

Structurei

3D structure databases

ProteinModelPortaliP0AFE4
SMRiP0AFE4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4L family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0713 LUCA
HOGENOMiHOG000066429
InParanoidiP0AFE4
KOiK00340
OMAiMLNACNL
PhylomeDBiP0AFE4

Family and domain databases

HAMAPiMF_01456 NDH1_NuoK, 1 hit
InterProiView protein in InterPro
IPR001133 NADH_UbQ_OxRdtase_chain4L/K
PANTHERiPTHR11434 PTHR11434, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPLQHGLIL AAILFVLGLT GLVIRRNLLF MLIGLEIMIN ASALAFVVAG
60 70 80 90 100
SYWGQTDGQV MYILAISLAA AEASIGLALL LQLHRRRQNL NIDSVSEMRG
Length:100
Mass (Da):10,845
Last modified:December 20, 2005 - v1
Checksum:iCC5AF3946A879383
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 41NA → RP in CAA48370 (PubMed:7690854).Curated2
Sequence conflicti46 – 59FVVAG…QTDGQ → LRGRRKLLGPRPTVK in CAA48370 (PubMed:7690854).CuratedAdd BLAST14
Sequence conflicti67S → T in CAA48370 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48370.1
U00096 Genomic DNA Translation: AAC75339.1
AP009048 Genomic DNA Translation: BAA16107.2
PIRiE64999
S38320 S37068
RefSeqiNP_416782.1, NC_000913.3
WP_000612644.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75339; AAC75339; b2279
BAA16107; BAA16107; BAA16107
GeneIDi947580
KEGGiecj:JW2274
eco:b2279
PATRICifig|1411691.4.peg.4457

Similar proteinsi

Entry informationi

Entry nameiNUOK_ECOLI
AccessioniPrimary (citable) accession number: P0AFE4
Secondary accession number(s): P33606, P76487, P78182
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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