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Protein

NADH-quinone oxidoreductase subunit K

Gene

nuoK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy-5-methyl-6-decyl-1,4-benzoquinone) as substrates.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOK-MONOMER.
ECOL316407:JW2274-MONOMER.
MetaCyc:NUOK-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit KUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit KUniRule annotation
NDH-1 subunit KUniRule annotation
NUO11
Gene namesi
Name:nuoKUniRule annotation
Ordered Locus Names:b2279, JW2274
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12091. nuoK.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3PeriplasmicSequence analysis3
Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21
Topological domaini25 – 27CytoplasmicSequence analysis3
Transmembranei28 – 48HelicalUniRule annotationAdd BLAST21
Topological domaini49 – 59PeriplasmicSequence analysisAdd BLAST11
Transmembranei60 – 80HelicalUniRule annotationAdd BLAST21
Topological domaini81 – 100CytoplasmicSequence analysisAdd BLAST20

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25 – 26RR → AA: Retains 14% dNADH oxidase and dNADH-DB reductase activity. 1 Publication2
Mutagenesisi36E → A: Retains 2% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1
Mutagenesisi36E → Q: Retains 2% dNADH oxidase and dNADH-DB reductase activity, neither a membrane potential nor a proton gradient can be formed by isolated membranes. 1 Publication1
Mutagenesisi72E → A: Retains 43% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1
Mutagenesisi72E → Q: Retains 22% dNADH oxidase and dNADH-DB reductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185241 – 100NADH-quinone oxidoreductase subunit KAdd BLAST100

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFE4.
PRIDEiP0AFE4.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260508. 39 interactors.
DIPiDIP-51165N.
IntActiP0AFE4. 1 interactor.
STRINGi511145.b2279.

Structurei

3D structure databases

ProteinModelPortaliP0AFE4.
SMRiP0AFE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4L family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0713. LUCA.
HOGENOMiHOG000066429.
InParanoidiP0AFE4.
KOiK00340.
OMAiNFVAFSY.
PhylomeDBiP0AFE4.

Family and domain databases

HAMAPiMF_01456. NDH1_NuoK. 1 hit.
InterProiIPR001133. NADH_UbQ_OxRdtase_chain4L/K.
[Graphical view]
PANTHERiPTHR11434. PTHR11434. 1 hit.
PfamiPF00420. Oxidored_q2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPLQHGLIL AAILFVLGLT GLVIRRNLLF MLIGLEIMIN ASALAFVVAG
60 70 80 90 100
SYWGQTDGQV MYILAISLAA AEASIGLALL LQLHRRRQNL NIDSVSEMRG
Length:100
Mass (Da):10,845
Last modified:December 20, 2005 - v1
Checksum:iCC5AF3946A879383
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 41NA → RP in CAA48370 (PubMed:7690854).Curated2
Sequence conflicti46 – 59FVVAG…QTDGQ → LRGRRKLLGPRPTVK in CAA48370 (PubMed:7690854).CuratedAdd BLAST14
Sequence conflicti67S → T in CAA48370 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48370.1.
U00096 Genomic DNA. Translation: AAC75339.1.
AP009048 Genomic DNA. Translation: BAA16107.2.
PIRiE64999.
S38320. S37068.
RefSeqiNP_416782.1. NC_000913.3.
WP_000612644.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75339; AAC75339; b2279.
BAA16107; BAA16107; BAA16107.
GeneIDi947580.
KEGGiecj:JW2274.
eco:b2279.
PATRICi32119925. VBIEscCol129921_2372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48370.1.
U00096 Genomic DNA. Translation: AAC75339.1.
AP009048 Genomic DNA. Translation: BAA16107.2.
PIRiE64999.
S38320. S37068.
RefSeqiNP_416782.1. NC_000913.3.
WP_000612644.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFE4.
SMRiP0AFE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260508. 39 interactors.
DIPiDIP-51165N.
IntActiP0AFE4. 1 interactor.
STRINGi511145.b2279.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFE4.
PRIDEiP0AFE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75339; AAC75339; b2279.
BAA16107; BAA16107; BAA16107.
GeneIDi947580.
KEGGiecj:JW2274.
eco:b2279.
PATRICi32119925. VBIEscCol129921_2372.

Organism-specific databases

EchoBASEiEB2015.
EcoGeneiEG12091. nuoK.

Phylogenomic databases

eggNOGiCOG0713. LUCA.
HOGENOMiHOG000066429.
InParanoidiP0AFE4.
KOiK00340.
OMAiNFVAFSY.
PhylomeDBiP0AFE4.

Enzyme and pathway databases

BioCyciEcoCyc:NUOK-MONOMER.
ECOL316407:JW2274-MONOMER.
MetaCyc:NUOK-MONOMER.

Miscellaneous databases

PROiP0AFE4.

Family and domain databases

HAMAPiMF_01456. NDH1_NuoK. 1 hit.
InterProiIPR001133. NADH_UbQ_OxRdtase_chain4L/K.
[Graphical view]
PANTHERiPTHR11434. PTHR11434. 1 hit.
PfamiPF00420. Oxidored_q2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOK_ECOLI
AccessioniPrimary (citable) accession number: P0AFE4
Secondary accession number(s): P33606, P76487, P78182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.