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Protein

NADH-quinone oxidoreductase subunit J

Gene

nuoJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOJ-MONOMER.
ECOL316407:JW2275-MONOMER.
MetaCyc:NUOJ-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit J (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit J
NDH-1 subunit J
NUO10
Gene namesi
Name:nuoJ
Ordered Locus Names:b2280, JW2275
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12090. nuoJ.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Topological domaini22 – 27CytoplasmicSequence analysis6
Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
Topological domaini49 – 53PeriplasmicSequence analysis5
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 91CytoplasmicSequence analysisAdd BLAST17
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21
Topological domaini113 – 137PeriplasmicSequence analysisAdd BLAST25
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 184CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001183701 – 184NADH-quinone oxidoreductase subunit JAdd BLAST184

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFE0.
PRIDEiP0AFE0.

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260509. 26 interactors.
DIPiDIP-59258N.
STRINGi511145.b2280.

Structurei

3D structure databases

ProteinModelPortaliP0AFE0.
SMRiP0AFE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 6 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107UFS. Bacteria.
COG0839. LUCA.
HOGENOMiHOG000262591.
InParanoidiP0AFE0.
KOiK00339.
OMAiIGVRHIG.
PhylomeDBiP0AFE0.

Family and domain databases

InterProiIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
[Graphical view]
PfamiPF00499. Oxidored_q3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFAFYICGL IAILATLRVI THTNPVHALL YLIISLLAIS GVFFSLGAYF
60 70 80 90 100
AGALEIIVYA GAIMVLFVFV VMMLNLGGSE IEQERQWLKP QVWIGPAILS
110 120 130 140 150
AIMLVVIVYA ILGVNDQGID GTPISAKAVG ITLFGPYVLA VELASMLLLA
160 170 180
GLVVAFHVGR EERAGEVLSN RKDDSAKRKT EEHA
Length:184
Mass (Da):19,875
Last modified:December 20, 2005 - v1
Checksum:i9C9D0C9ABAEC7755
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85R → T in CAA48369 (PubMed:7690854).Curated1
Sequence conflicti126A → R in CAA48369 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48369.1.
U00096 Genomic DNA. Translation: AAC75340.1.
AP009048 Genomic DNA. Translation: BAA16108.1.
PIRiF64999.
RefSeqiNP_416783.1. NC_000913.3.
WP_000393511.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75340; AAC75340; b2280.
BAA16108; BAA16108; BAA16108.
GeneIDi946756.
KEGGiecj:JW2275.
eco:b2280.
PATRICi32119927. VBIEscCol129921_2373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48369.1.
U00096 Genomic DNA. Translation: AAC75340.1.
AP009048 Genomic DNA. Translation: BAA16108.1.
PIRiF64999.
RefSeqiNP_416783.1. NC_000913.3.
WP_000393511.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFE0.
SMRiP0AFE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260509. 26 interactors.
DIPiDIP-59258N.
STRINGi511145.b2280.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFE0.
PRIDEiP0AFE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75340; AAC75340; b2280.
BAA16108; BAA16108; BAA16108.
GeneIDi946756.
KEGGiecj:JW2275.
eco:b2280.
PATRICi32119927. VBIEscCol129921_2373.

Organism-specific databases

EchoBASEiEB2014.
EcoGeneiEG12090. nuoJ.

Phylogenomic databases

eggNOGiENOG4107UFS. Bacteria.
COG0839. LUCA.
HOGENOMiHOG000262591.
InParanoidiP0AFE0.
KOiK00339.
OMAiIGVRHIG.
PhylomeDBiP0AFE0.

Enzyme and pathway databases

BioCyciEcoCyc:NUOJ-MONOMER.
ECOL316407:JW2275-MONOMER.
MetaCyc:NUOJ-MONOMER.

Miscellaneous databases

PROiP0AFE0.

Family and domain databases

InterProiIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
[Graphical view]
PfamiPF00499. Oxidored_q3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOJ_ECOLI
AccessioniPrimary (citable) accession number: P0AFE0
Secondary accession number(s): P33605, P78236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.