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Protein

NADH-quinone oxidoreductase subunit I

Gene

nuoI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi66Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi70Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi99Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi102Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi105Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi109Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOI-MONOMER.
ECOL316407:JW2276-MONOMER.
MetaCyc:NUOI-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit I (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit I
NDH-1 subunit I
NUO9
Gene namesi
Name:nuoI
Ordered Locus Names:b2281, JW2276
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12089. nuoI.

Subcellular locationi

GO - Cellular componenti

  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187221 – 180NADH-quinone oxidoreductase subunit IAdd BLAST180

Keywords - PTMi

Quinone

Proteomic databases

EPDiP0AFD6.
PaxDbiP0AFD6.
PRIDEiP0AFD6.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-35918N.
IntActiP0AFD6. 7 interactors.
STRINGi511145.b2281.

Structurei

3D structure databases

ProteinModelPortaliP0AFD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 804Fe-4S ferredoxin-type 1Add BLAST31
Domaini90 – 1194Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1143. LUCA.
HOGENOMiHOG000228291.
InParanoidiP0AFD6.
KOiK00338.
OMAiWYPDFFR.
PhylomeDBiP0AFD6.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKELLVGF GTQVRSIWMI GLHAFAKRET RMYPEEPVYL PPRYRGRIVL
60 70 80 90 100
TRDPDGEERC VACNLCAVAC PVGCISLQKA ETKDGRWYPE FFRINFSRCI
110 120 130 140 150
FCGLCEEACP TTAIQLTPDF EMGEYKRQDL VYEKEDLLIS GPGKYPEYNF
160 170 180
YRMAGMAIDG KDKGEAENEA KPIDVKSLLP
Length:180
Mass (Da):20,538
Last modified:December 20, 2005 - v1
Checksum:iB31860402432C171
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 29AFAKRE → GSPNQ in CAA48368 (PubMed:7690854).Curated6
Sequence conflicti165 – 175EAENEAKPIDV → DRRTKPSLSTF in CAA48368 (PubMed:7690854).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48368.1.
U00096 Genomic DNA. Translation: AAC75341.1.
AP009048 Genomic DNA. Translation: BAA16109.2.
PIRiG64999.
RefSeqiNP_416784.1. NC_000913.3.
WP_000172749.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75341; AAC75341; b2281.
BAA16109; BAA16109; BAA16109.
GeneIDi946757.
KEGGiecj:JW2276.
eco:b2281.
PATRICi32119929. VBIEscCol129921_2374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48368.1.
U00096 Genomic DNA. Translation: AAC75341.1.
AP009048 Genomic DNA. Translation: BAA16109.2.
PIRiG64999.
RefSeqiNP_416784.1. NC_000913.3.
WP_000172749.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35918N.
IntActiP0AFD6. 7 interactors.
STRINGi511145.b2281.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

EPDiP0AFD6.
PaxDbiP0AFD6.
PRIDEiP0AFD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75341; AAC75341; b2281.
BAA16109; BAA16109; BAA16109.
GeneIDi946757.
KEGGiecj:JW2276.
eco:b2281.
PATRICi32119929. VBIEscCol129921_2374.

Organism-specific databases

EchoBASEiEB2013.
EcoGeneiEG12089. nuoI.

Phylogenomic databases

eggNOGiCOG1143. LUCA.
HOGENOMiHOG000228291.
InParanoidiP0AFD6.
KOiK00338.
OMAiWYPDFFR.
PhylomeDBiP0AFD6.

Enzyme and pathway databases

BioCyciEcoCyc:NUOI-MONOMER.
ECOL316407:JW2276-MONOMER.
MetaCyc:NUOI-MONOMER.

Miscellaneous databases

PROiP0AFD6.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOI_ECOLI
AccessioniPrimary (citable) accession number: P0AFD6
Secondary accession number(s): P33604, P76488, P78183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.