P0AFD4 (NUOH_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 61.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NADH-quinone oxidoreductase subunit H EC=1.6.99.5 Alternative name(s): NADH dehydrogenase I subunit H NDH-1 subunit H NUO8 | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 325 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone. HAMAP MF_01350 |
| Catalytic activity | NADH + quinone = NAD+ + quinol. HAMAP MF_01350 |
| Subunit structure | NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex. |
| Subcellular location | Cell inner membrane; Multi-pass membrane protein HAMAP MF_01350. |
| Sequence similarities | Belongs to the complex I subunit 1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell inner membrane Cell membrane Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | NAD Ubiquinone |
| Molecular function | Oxidoreductase |
| PTM | Quinone |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | aerobic respiration Inferred from mutant phenotype. Source: EcoCyc anaerobic respirationInferred from mutant phenotype. Source: EcoliWiki |
| Cellular component | integral to plasma membrane Inferred from direct assay Ref.5. Source: EcoCyc plasma membrane respiratory chain complex IInferred from direct assay. Source: EcoCyc |
| Molecular function | NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC oxidoreduction-driven active transmembrane transporter activityInferred from direct assay. Source: EcoliWiki quinone bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 325 | 325 | NADH-quinone oxidoreductase subunit H HAMAP MF_01350 | PRO_0000117523 | |||||
Regions | |||||||||
| Transmembrane | 11 – 31 | 21 | Helical; Potential | ||||||
| Transmembrane | 81 – 101 | 21 | Helical; Potential | ||||||
| Transmembrane | 114 – 134 | 21 | Helical; Potential | ||||||
| Transmembrane | 154 – 174 | 21 | Helical; Potential | ||||||
| Transmembrane | 186 – 206 | 21 | Helical; Potential | ||||||
| Transmembrane | 237 – 257 | 21 | Helical; Potential | ||||||
| Transmembrane | 265 – 285 | 21 | Helical; Potential | ||||||
| Transmembrane | 304 – 324 | 21 | Helical; Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 35 – 36 | 2 | GE → AK in CAA48367. Ref.1 | ||||||
| Sequence conflict | 53 | 1 | G → A in CAA48367. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387. |
| [2] | "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T.DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X68301 Genomic DNA. Translation: CAA48367.1. U00096 Genomic DNA. Translation: AAC75342.1. AP009048 Genomic DNA. Translation: BAA16110.1. |
| PIR | H64999. |
| RefSeq | NP_416785.1. NC_000913.2. |
3D structure databases | |
| ProteinModelPortal | P0AFD4. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P0AFD4. 1 interaction. |
Protein family/group databases | |
| TCDB | 3.D.1.1.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000002147; EBESCP00000002147; EBESCG00000001759. EBESCT00000002148; EBESCP00000002148; EBESCG00000001759. EBESCT00000017553; EBESCP00000016844; EBESCG00000016609. |
| GeneID | 946761. |
| GenomeReviews | Gene locus JW2277 in contig AP009048_GR. Gene locus b2282 in contig U00096_GR. |
| KEGG | ecj:JW2277. eco:b2282. |
| PATRIC | 32119931. VBIEscCol129921_2375. |
Organism-specific databases | |
| EchoBASE | EB2012. |
| EcoGene | EG12088. nuoH. |
Phylogenomic databases | |
| eggNOG | COG1005. |
| GeneTree | EBGT00050000009143. |
| HOGENOM | HBG727670. |
| OMA | MSWLTPE. |
| PhylomeDB | P0AFD4. |
| ProtClustDB | PRK06076. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:NUOH-MONOMER. MetaCyc:NUOH-MONOMER. |
Gene expression databases | |
| Genevestigator | P0AFD4. |
Family and domain databases | |
| HAMAP | MF_01350. NDH1_NuoH. [Tree] |
| InterPro | IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view] |
| KO | K00337. |
| PANTHER | PTHR11432. Resp_NADH_DH_1. 1 hit. |
| Pfam | PF00146. NADHdh. 1 hit. [Graphical view] |
| PROSITE | PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NUOH_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0AFD4 Secondary accession number(s): P33603, P78307 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

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