P0AFD4 (NUOH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H
NUO8

Gene names

Name:	nuoH
Ordered Locus Names:	b2282, JW2277

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype. Source: EcoCyc anaerobic respiration Inferred from mutant phenotype. Source: EcoliWiki
Cellular component	integral to plasma membrane Inferred from direct assay Ref.5. Source: EcoCyc plasma membrane respiratory chain complex I Inferred from direct assay. Source: EcoCyc
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC oxidoreduction-driven active transmembrane transporter activity Inferred from direct assay. Source: EcoliWiki quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000117523
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Transmembrane	304 – 324	21	Helical; Potential
Experimental info
	Sequence conflict	35 – 36	2	GE → AK in CAA48367. Ref.1
	Sequence conflict	53	1	G → A in CAA48367. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AFD4 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 0648FD831E1B8FB0	FASTA	325	36,219
	10 20 30 40 50 60 MSWISPELIE ILLTILKAVV ILLVVVTCGA FMSFGERRLL GLFQNRYGPN RVGWGGSLQL 70 80 90 100 110 120 VADMIKMFFK EDWIPKFSDR VIFTLAPMIA FTSLLLAFAI VPVSPGWVVA DLNIGILFFL 130 140 150 160 170 180 MMAGLAVYAV LFAGWSSNNK YSLLGAMRAS AQTLSYEVFL GLSLMGVVAQ AGSFNMTDIV 190 200 210 220 230 240 NSQAHVWNVI PQFFGFITFA IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG 250 260 270 280 290 300 EYIGIVTISA LMVTLFFGGW QGPLLPPFIW FALKTAFFMM MFILIRASLP RPRYDQVMSF 310 320 GWKICLPLTL INLLVTAAVI LWQAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68301 Genomic DNA. Translation: CAA48367.1. U00096 Genomic DNA. Translation: AAC75342.1. AP009048 Genomic DNA. Translation: BAA16110.1.
PIR	H64999.
RefSeq	NP_416785.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AFD4.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AFD4. 1 interaction.
Protein family/group databases
TCDB	3.D.1.1.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002147; EBESCP00000002147; EBESCG00000001759. EBESCT00000002148; EBESCP00000002148; EBESCG00000001759. EBESCT00000017553; EBESCP00000016844; EBESCG00000016609.
GeneID	946761.
GenomeReviews	Gene locus JW2277 in contig AP009048_GR. Gene locus b2282 in contig U00096_GR.
KEGG	ecj:JW2277. eco:b2282.
PATRIC	32119931. VBIEscCol129921_2375.
Organism-specific databases
EchoBASE	EB2012.
EcoGene	EG12088. nuoH.
Phylogenomic databases
eggNOG	COG1005.
GeneTree	EBGT00050000009143.
HOGENOM	HBG727670.
OMA	MSWLTPE.
PhylomeDB	P0AFD4.
ProtClustDB	PRK06076.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOH-MONOMER. MetaCyc:NUOH-MONOMER.
Gene expression databases
Genevestigator	P0AFD4.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K00337.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_ECOLI

Accession

Primary (citable) accession number: P0AFD4
Secondary accession number(s): P33603, P78307

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program