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Protein

NADH-quinone oxidoreductase subunit H

Gene

nuoH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOH-MONOMER
MetaCyc:NUOH-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit H (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H
NUO8
Gene namesi
Name:nuoH
Ordered Locus Names:b2282, JW2277
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12088 nuoH

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Transmembranei304 – 324HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001175231 – 325NADH-quinone oxidoreductase subunit HAdd BLAST325

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFD4
PRIDEiP0AFD4

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260511, 40 interactors
IntActiP0AFD4, 1 interactor
STRINGi316385.ECDH10B_2444

Structurei

3D structure databases

ProteinModelPortaliP0AFD4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMZ Bacteria
COG1005 LUCA
HOGENOMiHOG000228276
InParanoidiP0AFD4
KOiK00337
OMAiWNIWRQP
PhylomeDBiP0AFD4

Family and domain databases

HAMAPiMF_01350 NDH1_NuoH, 1 hit
InterProiView protein in InterPro
IPR001694 NADH_UbQ_OxRdtase_su1/FPO
IPR018086 NADH_UbQ_OxRdtase_su1_CS
PANTHERiPTHR11432 PTHR11432, 1 hit
PfamiView protein in Pfam
PF00146 NADHdh, 1 hit
PROSITEiView protein in PROSITE
PS00667 COMPLEX1_ND1_1, 1 hit
PS00668 COMPLEX1_ND1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWISPELIE ILLTILKAVV ILLVVVTCGA FMSFGERRLL GLFQNRYGPN
60 70 80 90 100
RVGWGGSLQL VADMIKMFFK EDWIPKFSDR VIFTLAPMIA FTSLLLAFAI
110 120 130 140 150
VPVSPGWVVA DLNIGILFFL MMAGLAVYAV LFAGWSSNNK YSLLGAMRAS
160 170 180 190 200
AQTLSYEVFL GLSLMGVVAQ AGSFNMTDIV NSQAHVWNVI PQFFGFITFA
210 220 230 240 250
IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG EYIGIVTISA
260 270 280 290 300
LMVTLFFGGW QGPLLPPFIW FALKTAFFMM MFILIRASLP RPRYDQVMSF
310 320
GWKICLPLTL INLLVTAAVI LWQAQ
Length:325
Mass (Da):36,219
Last modified:December 20, 2005 - v1
Checksum:i0648FD831E1B8FB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36GE → AK in CAA48367 (PubMed:7690854).Curated2
Sequence conflicti53G → A in CAA48367 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48367.1
U00096 Genomic DNA Translation: AAC75342.1
AP009048 Genomic DNA Translation: BAA16110.1
PIRiH64999
RefSeqiNP_416785.1, NC_000913.3
WP_000118507.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75342; AAC75342; b2282
BAA16110; BAA16110; BAA16110
GeneIDi946761
KEGGiecj:JW2277
eco:b2282
PATRICifig|1411691.4.peg.4454

Similar proteinsi

Entry informationi

Entry nameiNUOH_ECOLI
AccessioniPrimary (citable) accession number: P0AFD4
Secondary accession number(s): P33603, P78307
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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