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Protein

NADH-quinone oxidoreductase subunit H

Gene

nuoH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOH-MONOMER.
ECOL316407:JW2277-MONOMER.
MetaCyc:NUOH-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit H (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H
NUO8
Gene namesi
Name:nuoH
Ordered Locus Names:b2282, JW2277
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12088. nuoH.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Transmembranei304 – 324HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001175231 – 325NADH-quinone oxidoreductase subunit HAdd BLAST325

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFD4.
PRIDEiP0AFD4.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4260511. 24 interactors.
IntActiP0AFD4. 1 interactor.
STRINGi511145.b2282.

Structurei

3D structure databases

ProteinModelPortaliP0AFD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMZ. Bacteria.
COG1005. LUCA.
HOGENOMiHOG000228276.
InParanoidiP0AFD4.
KOiK00337.
OMAiLDLGWKF.
PhylomeDBiP0AFD4.

Family and domain databases

HAMAPiMF_01350. NDH1_NuoH. 1 hit.
InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWISPELIE ILLTILKAVV ILLVVVTCGA FMSFGERRLL GLFQNRYGPN
60 70 80 90 100
RVGWGGSLQL VADMIKMFFK EDWIPKFSDR VIFTLAPMIA FTSLLLAFAI
110 120 130 140 150
VPVSPGWVVA DLNIGILFFL MMAGLAVYAV LFAGWSSNNK YSLLGAMRAS
160 170 180 190 200
AQTLSYEVFL GLSLMGVVAQ AGSFNMTDIV NSQAHVWNVI PQFFGFITFA
210 220 230 240 250
IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG EYIGIVTISA
260 270 280 290 300
LMVTLFFGGW QGPLLPPFIW FALKTAFFMM MFILIRASLP RPRYDQVMSF
310 320
GWKICLPLTL INLLVTAAVI LWQAQ
Length:325
Mass (Da):36,219
Last modified:December 20, 2005 - v1
Checksum:i0648FD831E1B8FB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36GE → AK in CAA48367 (PubMed:7690854).Curated2
Sequence conflicti53G → A in CAA48367 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48367.1.
U00096 Genomic DNA. Translation: AAC75342.1.
AP009048 Genomic DNA. Translation: BAA16110.1.
PIRiH64999.
RefSeqiNP_416785.1. NC_000913.3.
WP_000118507.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75342; AAC75342; b2282.
BAA16110; BAA16110; BAA16110.
GeneIDi946761.
KEGGiecj:JW2277.
eco:b2282.
PATRICi32119931. VBIEscCol129921_2375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48367.1.
U00096 Genomic DNA. Translation: AAC75342.1.
AP009048 Genomic DNA. Translation: BAA16110.1.
PIRiH64999.
RefSeqiNP_416785.1. NC_000913.3.
WP_000118507.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260511. 24 interactors.
IntActiP0AFD4. 1 interactor.
STRINGi511145.b2282.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFD4.
PRIDEiP0AFD4.

Protocols and materials databases

DNASUi946761.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75342; AAC75342; b2282.
BAA16110; BAA16110; BAA16110.
GeneIDi946761.
KEGGiecj:JW2277.
eco:b2282.
PATRICi32119931. VBIEscCol129921_2375.

Organism-specific databases

EchoBASEiEB2012.
EcoGeneiEG12088. nuoH.

Phylogenomic databases

eggNOGiENOG4105CMZ. Bacteria.
COG1005. LUCA.
HOGENOMiHOG000228276.
InParanoidiP0AFD4.
KOiK00337.
OMAiLDLGWKF.
PhylomeDBiP0AFD4.

Enzyme and pathway databases

BioCyciEcoCyc:NUOH-MONOMER.
ECOL316407:JW2277-MONOMER.
MetaCyc:NUOH-MONOMER.

Miscellaneous databases

PROiP0AFD4.

Family and domain databases

HAMAPiMF_01350. NDH1_NuoH. 1 hit.
InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOH_ECOLI
AccessioniPrimary (citable) accession number: P0AFD4
Secondary accession number(s): P33603, P78307
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.