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Protein

NADH-quinone oxidoreductase subunit E

Gene

nuoE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Iron-sulfur (2Fe-2S)Sequence analysis
Metal bindingi97 – 971Iron-sulfur (2Fe-2S)Sequence analysis
Metal bindingi133 – 1331Iron-sulfur (2Fe-2S)Sequence analysis
Metal bindingi137 – 1371Iron-sulfur (2Fe-2S)Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOE-MONOMER.
ECOL316407:JW2280-MONOMER.
MetaCyc:NUOE-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit E (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E
NUO5
Gene namesi
Name:nuoE
Ordered Locus Names:b2285, JW2280
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12086. nuoE.

Subcellular locationi

GO - Cellular componenti

  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 166166NADH-quinone oxidoreductase subunit EPRO_0000118691Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFD1.
PRIDEiP0AFD1.

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
nuoGP336024EBI-1117136,EBI-559737

Protein-protein interaction databases

BioGridi4262977. 15 interactions.
DIPiDIP-35917N.
IntActiP0AFD1. 31 interactions.
STRINGi511145.b2285.

Structurei

3D structure databases

ProteinModelPortaliP0AFD1.
SMRiP0AFD1. Positions 30-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105GKE. Bacteria.
COG1905. LUCA.
HOGENOMiHOG000257749.
InParanoidiP0AFD1.
KOiK00334.
OMAiCDKGPSM.
PhylomeDBiP0AFD1.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHENQQPQTE AFELSAAERE AIEHEMHHYE DPRAASIEAL KIVQKQRGWV
60 70 80 90 100
PDGAIHAIAD VLGIPASDVE GVATFYSQIF RQPVGRHVIR YCDSVVCHIN
110 120 130 140 150
GYQGIQAALE KKLNIKPGQT TFDGRFTLLP TCCLGNCDKG PNMMIDEDTH
160
AHLTPEAIPE LLERYK
Length:166
Mass (Da):18,590
Last modified:December 20, 2005 - v1
Checksum:iA1EA95085B9B9107
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221I → V in CAA48364 (PubMed:7690854).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48364.1.
L25055 Unassigned DNA. Translation: AAA03536.1.
U00096 Genomic DNA. Translation: AAC75345.1.
AP009048 Genomic DNA. Translation: BAA16114.1.
PIRiC65000.
RefSeqiNP_416788.1. NC_000913.3.
WP_000545042.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75345; AAC75345; b2285.
BAA16114; BAA16114; BAA16114.
GeneIDi946746.
KEGGiecj:JW2280.
eco:b2285.
PATRICi32119937. VBIEscCol129921_2378.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48364.1.
L25055 Unassigned DNA. Translation: AAA03536.1.
U00096 Genomic DNA. Translation: AAC75345.1.
AP009048 Genomic DNA. Translation: BAA16114.1.
PIRiC65000.
RefSeqiNP_416788.1. NC_000913.3.
WP_000545042.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFD1.
SMRiP0AFD1. Positions 30-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262977. 15 interactions.
DIPiDIP-35917N.
IntActiP0AFD1. 31 interactions.
STRINGi511145.b2285.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFD1.
PRIDEiP0AFD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75345; AAC75345; b2285.
BAA16114; BAA16114; BAA16114.
GeneIDi946746.
KEGGiecj:JW2280.
eco:b2285.
PATRICi32119937. VBIEscCol129921_2378.

Organism-specific databases

EchoBASEiEB2010.
EcoGeneiEG12086. nuoE.

Phylogenomic databases

eggNOGiENOG4105GKE. Bacteria.
COG1905. LUCA.
HOGENOMiHOG000257749.
InParanoidiP0AFD1.
KOiK00334.
OMAiCDKGPSM.
PhylomeDBiP0AFD1.

Enzyme and pathway databases

BioCyciEcoCyc:NUOE-MONOMER.
ECOL316407:JW2280-MONOMER.
MetaCyc:NUOE-MONOMER.

Miscellaneous databases

PROiP0AFD1.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOE_ECOLI
AccessioniPrimary (citable) accession number: P0AFD1
Secondary accession number(s): P33601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.