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Protein

NADH-quinone oxidoreductase subunit E

Gene

nuoE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi97Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi133Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi137Iron-sulfur (2Fe-2S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOE-MONOMER
MetaCyc:NUOE-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit E (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E
NUO5
Gene namesi
Name:nuoE
Ordered Locus Names:b2285, JW2280
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12086 nuoE

Subcellular locationi

GO - Cellular componenti

  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001186911 – 166NADH-quinone oxidoreductase subunit EAdd BLAST166

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFD1
PRIDEiP0AFD1

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
nuoGP336024EBI-1117136,EBI-559737

Protein-protein interaction databases

BioGridi4262977, 41 interactors
DIPiDIP-35917N
IntActiP0AFD1, 31 interactors
STRINGi316385.ECDH10B_2447

Structurei

3D structure databases

ProteinModelPortaliP0AFD1
SMRiP0AFD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105GKE Bacteria
COG1905 LUCA
HOGENOMiHOG000257749
InParanoidiP0AFD1
KOiK00334
OMAiYHLFSLK
PhylomeDBiP0AFD1

Family and domain databases

CDDicd03064 TRX_Fd_NuoE, 1 hit
InterProiView protein in InterPro
IPR002023 NuoE-like
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR10371 PTHR10371, 1 hit
PIRSFiPIRSF000216 NADH_DH_24kDa, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01958 nuoE_fam, 1 hit
PROSITEiView protein in PROSITE
PS01099 COMPLEX1_24K, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHENQQPQTE AFELSAAERE AIEHEMHHYE DPRAASIEAL KIVQKQRGWV
60 70 80 90 100
PDGAIHAIAD VLGIPASDVE GVATFYSQIF RQPVGRHVIR YCDSVVCHIN
110 120 130 140 150
GYQGIQAALE KKLNIKPGQT TFDGRFTLLP TCCLGNCDKG PNMMIDEDTH
160
AHLTPEAIPE LLERYK
Length:166
Mass (Da):18,590
Last modified:December 20, 2005 - v1
Checksum:iA1EA95085B9B9107
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22I → V in CAA48364 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48364.1
L25055 Unassigned DNA Translation: AAA03536.1
U00096 Genomic DNA Translation: AAC75345.1
AP009048 Genomic DNA Translation: BAA16114.1
PIRiC65000
RefSeqiNP_416788.1, NC_000913.3
WP_000545042.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75345; AAC75345; b2285
BAA16114; BAA16114; BAA16114
GeneIDi946746
KEGGiecj:JW2280
eco:b2285
PATRICifig|1411691.4.peg.4451

Similar proteinsi

Entry informationi

Entry nameiNUOE_ECOLI
AccessioniPrimary (citable) accession number: P0AFD1
Secondary accession number(s): P33601
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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