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Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi64 – 641Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi129 – 1291Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi158 – 1581Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron ion binding Source: UniProtKB-HAMAP
  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • electron transport coupled proton transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOB-MONOMER.
ECOL316407:JW5875-MONOMER.
MetaCyc:NUOB-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit BUniRule annotation
NDH-1 subunit BUniRule annotation
NUO2
Gene namesi
Name:nuoBUniRule annotation
Ordered Locus Names:b2287, JW5875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12083. nuoB.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220NADH-quinone oxidoreductase subunit BPRO_0000118765Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

EPDiP0AFC7.
PaxDbiP0AFC7.
PRIDEiP0AFC7.

2D gel databases

SWISS-2DPAGEP0AFC7.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

BioGridi4262975. 12 interactions.
IntActiP0AFC7. 1 interaction.
STRINGi511145.b2287.

Structurei

3D structure databases

ProteinModelPortaliP0AFC7.
SMRiP0AFC7. Positions 35-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ES3. Bacteria.
COG0377. LUCA.
HOGENOMiHOG000228249.
InParanoidiP0AFC7.
KOiK00331.
OMAiPRPEAYI.
OrthoDBiEOG62K20C.
PhylomeDBiP0AFC7.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR
60 70 80 90 100
KNSIWPYNFG LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG
110 120 130 140 150
TCFTKMAPVI QRLYDQMLEP KWVISMGACA NSGGMYDIYS VVQGVDKFIP
160 170 180 190 200
VDVYIPGCPP RPEAYMQALM LLQESIGKER RPLSWVVGDQ GVYRANMQSE
210 220
RERKRGERIA VTNLRTPDEI
Length:220
Mass (Da):25,056
Last modified:December 20, 2005 - v1
Checksum:iEBD6268505993880
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711S → L in CAA48361 (PubMed:7690854).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48361.1.
U00096 Genomic DNA. Translation: AAC75347.1.
AP009048 Genomic DNA. Translation: BAA16121.2.
PIRiE65000.
RefSeqiNP_416790.1. NC_000913.3.
WP_000386733.1. NZ_CP014272.1.
WP_046608242.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75347; AAC75347; b2287.
BAA16121; BAA16121; BAA16121.
GeneIDi946738.
KEGGiecj:JW5875.
eco:b2287.
PATRICi32119941. VBIEscCol129921_2380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48361.1.
U00096 Genomic DNA. Translation: AAC75347.1.
AP009048 Genomic DNA. Translation: BAA16121.2.
PIRiE65000.
RefSeqiNP_416790.1. NC_000913.3.
WP_000386733.1. NZ_CP014272.1.
WP_046608242.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFC7.
SMRiP0AFC7. Positions 35-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262975. 12 interactions.
IntActiP0AFC7. 1 interaction.
STRINGi511145.b2287.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

2D gel databases

SWISS-2DPAGEP0AFC7.

Proteomic databases

EPDiP0AFC7.
PaxDbiP0AFC7.
PRIDEiP0AFC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75347; AAC75347; b2287.
BAA16121; BAA16121; BAA16121.
GeneIDi946738.
KEGGiecj:JW5875.
eco:b2287.
PATRICi32119941. VBIEscCol129921_2380.

Organism-specific databases

EchoBASEiEB2008.
EcoGeneiEG12083. nuoB.

Phylogenomic databases

eggNOGiENOG4105ES3. Bacteria.
COG0377. LUCA.
HOGENOMiHOG000228249.
InParanoidiP0AFC7.
KOiK00331.
OMAiPRPEAYI.
OrthoDBiEOG62K20C.
PhylomeDBiP0AFC7.

Enzyme and pathway databases

BioCyciEcoCyc:NUOB-MONOMER.
ECOL316407:JW5875-MONOMER.
MetaCyc:NUOB-MONOMER.

Miscellaneous databases

PROiP0AFC7.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I."
    Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H.
    J. Mol. Biol. 233:109-122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / AN387.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  6. "Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli."
    Leif H., Sled V.D., Ohnishi T., Weiss H., Friedrich T.
    Eur. J. Biochem. 230:538-548(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-6.

Entry informationi

Entry nameiNUOB_ECOLI
AccessioniPrimary (citable) accession number: P0AFC7
Secondary accession number(s): P33598
, P78090, P78186, P78187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.