Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi64Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi129Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi158Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • electron transport coupled proton transport Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processTransport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOB-MONOMER
MetaCyc:NUOB-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit BUniRule annotation
NDH-1 subunit BUniRule annotation
NUO2
Gene namesi
Name:nuoBUniRule annotation
Ordered Locus Names:b2287, JW5875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12083 nuoB

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187651 – 220NADH-quinone oxidoreductase subunit BAdd BLAST220

Keywords - PTMi

Quinone

Proteomic databases

EPDiP0AFC7
PaxDbiP0AFC7
PRIDEiP0AFC7

2D gel databases

SWISS-2DPAGEiP0AFC7

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

BioGridi4262975, 59 interactors
IntActiP0AFC7, 1 interactor
STRINGi316385.ECDH10B_2449

Structurei

3D structure databases

ProteinModelPortaliP0AFC7
SMRiP0AFC7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ES3 Bacteria
COG0377 LUCA
HOGENOMiHOG000228249
InParanoidiP0AFC7
KOiK00331
OMAiGPYWEHG
PhylomeDBiP0AFC7

Family and domain databases

HAMAPiMF_01356 NDH1_NuoB, 1 hit
InterProiView protein in InterPro
IPR006137 NADH_UbQ_OxRdtase-like_20kDa
IPR006138 NADH_UQ_OxRdtase_20Kd_su
PfamiView protein in Pfam
PF01058 Oxidored_q6, 1 hit
TIGRFAMsiTIGR01957 nuoB_fam, 1 hit
PROSITEiView protein in PROSITE
PS01150 COMPLEX1_20K, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR
60 70 80 90 100
KNSIWPYNFG LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG
110 120 130 140 150
TCFTKMAPVI QRLYDQMLEP KWVISMGACA NSGGMYDIYS VVQGVDKFIP
160 170 180 190 200
VDVYIPGCPP RPEAYMQALM LLQESIGKER RPLSWVVGDQ GVYRANMQSE
210 220
RERKRGERIA VTNLRTPDEI
Length:220
Mass (Da):25,056
Last modified:December 20, 2005 - v1
Checksum:iEBD6268505993880
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71S → L in CAA48361 (PubMed:7690854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48361.1
U00096 Genomic DNA Translation: AAC75347.1
AP009048 Genomic DNA Translation: BAA16121.2
PIRiE65000
RefSeqiNP_416790.1, NC_000913.3
WP_000386733.1, NZ_CP014272.1
WP_046608242.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75347; AAC75347; b2287
BAA16121; BAA16121; BAA16121
GeneIDi946738
KEGGiecj:JW5875
eco:b2287
PATRICifig|1411691.4.peg.4449

Similar proteinsi

Entry informationi

Entry nameiNUOB_ECOLI
AccessioniPrimary (citable) accession number: P0AFC7
Secondary accession number(s): P33598
, P78090, P78186, P78187
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health