NADH-quinone oxidoreductase subunit B - Escherichia coli (strain K12)

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P0AFC7 (NUOB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit B
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit B
NDH-1 subunit B
NUO2

Gene names

Name:	nuoB
Ordered Locus Names:	b2287, JW5875

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01356
Catalytic activity	NADH + quinone = NAD⁺ + quinol. HAMAP MF_01356
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01356
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side HAMAP MF_01356.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype. Source: EcoCyc anaerobic respiration Inferred from mutant phenotype. Source: EcoliWiki electron transport coupled proton transport Inferred from mutant phenotype. Source: EcoCyc
Cellular component	plasma membrane respiratory chain complex I Inferred from direct assay. Source: EcoCyc
Molecular function	4 iron, 4 sulfur cluster binding Inferred from mutant phenotype. Source: EcoCyc NADH dehydrogenase (ubiquinone) activity Inferred from mutant phenotype. Source: EcoCyc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreduction-driven active transmembrane transporter activity Inferred from direct assay. Source: EcoliWiki quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

NADH-quinone oxidoreductase subunit B HAMAP MF_01356

PRO_0000118765

Sites

Metal binding

Iron-sulfur (4Fe-4S) Potential

Metal binding

Iron-sulfur (4Fe-4S) Potential

Metal binding

129

Iron-sulfur (4Fe-4S) Potential

Metal binding

158

Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict

S → L in CAA48361. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFC7 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: EBD6268505993880
Show »

FASTA

220

25,056

        10         20         30         40         50         60 
MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR KNSIWPYNFG 

        70         80         90        100        110        120 
LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG TCFTKMAPVI QRLYDQMLEP 

       130        140        150        160        170        180 
KWVISMGACA NSGGMYDIYS VVQGVDKFIP VDVYIPGCPP RPEAYMQALM LLQESIGKER 

       190        200        210        220 
RPLSWVVGDQ GVYRANMQSE RERKRGERIA VTNLRTPDEI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[6]	"Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Leif H., Sled V.D., Ohnishi T., Weiss H., Friedrich T. Eur. J. Biochem. 230:538-548(1995) [PubMed: 7607227] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68301 Genomic DNA. Translation: CAA48361.1. U00096 Genomic DNA. Translation: AAC75347.1. AP009048 Genomic DNA. Translation: BAA16121.2.
PIR	E65000.
RefSeq	NP_416790.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AFC7.
SMR	P0AFC7. Positions 34-181.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AFC7. 1 interaction.
Protein family/group databases
TCDB	3.D.1.1.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
2D gel databases
SWISS-2DPAGE	P0AFC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002390; EBESCP00000002390; EBESCG00000001951. EBESCT00000002391; EBESCP00000002391; EBESCG00000001951. EBESCT00000016093; EBESCP00000015384; EBESCG00000015153.
GeneID	946738.
GenomeReviews	Gene locus JW5875 in contig AP009048_GR. Gene locus b2287 in contig U00096_GR.
KEGG	ecj:JW5875. eco:b2287.
PATRIC	32119941. VBIEscCol129921_2380.
Organism-specific databases
EchoBASE	EB2008.
EcoGene	EG12083. nuoB.
Phylogenomic databases
eggNOG	COG0377.
GeneTree	EBGT00050000009829.
HOGENOM	HBG553221.
OMA	PRPEAYI.
PhylomeDB	P0AFC7.
ProtClustDB	PRK06411.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOB-MONOMER. MetaCyc:NUOB-MONOMER.
Gene expression databases
Genevestigator	P0AFC7.
Family and domain databases
HAMAP	MF_01356. NDH1_NuoB. [Tree]
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K00331.
PANTHER	PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOB_ECOLI

Accession

Primary (citable) accession number: P0AFC7
Secondary accession number(s): P33598

P78187

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents