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Protein

NADH-quinone oxidoreductase subunit A

Gene

nuoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processTransport
LigandNAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOA-MONOMER
MetaCyc:NUOA-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit AUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit AUniRule annotation
NDH-1 subunit AUniRule annotation
NUO1UniRule annotation
Gene namesi
Name:nuoAUniRule annotation
Ordered Locus Names:b2288, JW2283
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12082 nuoA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalUniRule annotationAdd BLAST21
Transmembranei68 – 88HelicalUniRule annotationAdd BLAST21
Transmembranei98 – 118HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001178621 – 147NADH-quinone oxidoreductase subunit AAdd BLAST147

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFC3
PRIDEiP0AFC3

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4262974, 132 interactors
DIPiDIP-59257N
IntActiP0AFC3, 5 interactors
STRINGi316385.ECDH10B_2450

Structurei

3D structure databases

ProteinModelPortaliP0AFC3
SMRiP0AFC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 3 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105KWU Bacteria
COG0838 LUCA
HOGENOMiHOG000100121
InParanoidiP0AFC3
KOiK00330
OMAiKGALEWH
PhylomeDBiP0AFC3

Family and domain databases

Gene3Di1.20.58.1610, 1 hit
HAMAPiMF_01394 NDH1_NuoA, 1 hit
InterProiView protein in InterPro
IPR023043 NAD(P)H_OxRDtase_bac/plastid
IPR000440 NADH_UbQ/plastoQ_OxRdtase_su3
IPR038430 NDAH_ubi_oxred_su3_sf
PANTHERiPTHR11058 PTHR11058, 1 hit
PfamiView protein in Pfam
PF00507 Oxidored_q4, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF
60 70 80 90 100
ESGIDSVGSA RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF
110 120 130 140
VEAAIFIFVL LAGLVYLVRI GALDWTPARS RRERMNPETN SIANRQR
Length:147
Mass (Da):16,457
Last modified:December 20, 2005 - v1
Checksum:i1F9E0804B2B0D1B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60A → G in CAA48360 (PubMed:7690854).Curated1
Sequence conflicti82 – 91ALYLFAWSTS → GAVSVRMVLL in CAA48360 (PubMed:7690854).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48360.1
U00096 Genomic DNA Translation: AAC75348.1
AP009048 Genomic DNA Translation: BAA16123.1
PIRiF65000
RefSeqiNP_416791.3, NC_000913.3
WP_000062997.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75348; AAC75348; b2288
BAA16123; BAA16123; BAA16123
GeneIDi946764
KEGGiecj:JW2283
eco:b2288
PATRICifig|1411691.4.peg.4448

Similar proteinsi

Entry informationi

Entry nameiNUOA_ECOLI
AccessioniPrimary (citable) accession number: P0AFC3
Secondary accession number(s): P33597, P77159
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health