P0AFC3 (NUOA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	b2288, JW2283

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP-Rule MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01394
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 21925501. Source: EcoCyc plasma membrane respiratory chain complex I Inferred from direct assay PubMed 11997136. Source: EcoCyc
Molecular_function	NADH dehydrogenase (ubiquinone) activity Inferred from mutant phenotype PubMed 8366049. Source: EcoCyc quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	NADH-quinone oxidoreductase subunit A HAMAP-Rule MF_01394		PRO_0000117862
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
Experimental info
	Sequence conflict	60	1	A → G in CAA48360. Ref.1
	Sequence conflict	82 – 91	10	ALYLFAWSTS → GAVSVRMVLL in CAA48360. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AFC3 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 1F9E0804B2B0D1B5	FASTA	147	16,457
	10 20 30 40 50 60 MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF ESGIDSVGSA 70 80 90 100 110 120 RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF VEAAIFIFVL LAGLVYLVRI 130 140 GALDWTPARS RRERMNPETN SIANRQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68301 Genomic DNA. Translation: CAA48360.1. U00096 Genomic DNA. Translation: AAC75348.1. AP009048 Genomic DNA. Translation: BAA16123.1.
PIR	F65000.
RefSeq	NP_416791.3. NC_000913.2. YP_490528.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFC3.
SMR	P0AFC3. Positions 15-126.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59257N.
STRING	511145.b2288.
Protein family/group databases
TCDB	3.D.1.1.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PaxDb	P0AFC3.
PRIDE	P0AFC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75348; AAC75348; b2288. BAA16123; BAA16123; BAA16123.
GeneID	12933980. 946764.
KEGG	ecj:Y75_p2252. eco:b2288.
PATRIC	32119943. VBIEscCol129921_2381.
Organism-specific databases
EchoBASE	EB2007.
EcoGene	EG12082. nuoA.
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HOG000100121.
KO	K00330.
OMA	WVGFIEA.
ProtClustDB	PRK06602.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOA-MONOMER. ECOL316407:JW2283-MONOMER. MetaCyc:NUOA-MONOMER.
Gene expression databases
Genevestigator	P0AFC3.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA.
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. PTHR11058. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_ECOLI

Accession

Primary (citable) accession number: P0AFC3
Secondary accession number(s): P33597, P77159

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program