Reviewed, UniProtKB/Swiss-Prot P0AFC3 (NUOA_ECOLI)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A
    NDH-1 subunit A
    NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	b2288, JW2283

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex. HAMAP MF_01394
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.5
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000117862
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	68 – 88	21	Potential
	Transmembrane	98 – 118	21	Potential
Experimental info
	Sequence conflict	60	1	A → G in CAA48360. Ref.1
	Sequence conflict	82 – 91	10	ALYLFAWSTS → GAVSVRMVLL in CAA48360. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AFC3-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 1F9E0804B2B0D1B5	FASTA	147	16,457
	10 20 30 40 50 60 MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF ESGIDSVGSA 70 80 90 100 110 120 RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF VEAAIFIFVL LAGLVYLVRI 130 140 GALDWTPARS RRERMNPETN SIANRQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68301 Genomic DNA. Translation: CAA48360.1. U00096 Genomic DNA. Translation: AAC75348.1. AP009048 Genomic DNA. Translation: BAA16123.1.
PIR	F65000.
RefSeq	AP_002886.1. NP_416791.3.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.1.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	946764.
GenomeReviews	Gene locus JW2283 in contig AP009048_GR. Gene locus b2288 in contig U00096_GR.
KEGG	ecj:JW2283. eco:b2288.
Organism-specific databases
EchoBASE	EB2007.
EcoGene	EG12082. nuoA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AFC3.
OMA	P0AFC3. FIEATIF.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOA-MON. MetaCyc:NUOA-MON.
Family and domain databases
HAMAP	MF_01394. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_ECOLI

Accession

Primary (citable) accession number: P0AFC3
Secondary accession number(s): P33597, P77159

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)