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Protein

NADH-quinone oxidoreductase subunit A

Gene

nuoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOA-MONOMER.
ECOL316407:JW2283-MONOMER.
MetaCyc:NUOA-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit AUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit AUniRule annotation
NDH-1 subunit AUniRule annotation
NUO1UniRule annotation
Gene namesi
Name:nuoAUniRule annotation
Ordered Locus Names:b2288, JW2283
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12082. nuoA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalUniRule annotationAdd
BLAST
Transmembranei68 – 8821HelicalUniRule annotationAdd
BLAST
Transmembranei98 – 11821HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147NADH-quinone oxidoreductase subunit APRO_0000117862Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP0AFC3.
PRIDEiP0AFC3.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

BioGridi4262974. 23 interactions.
DIPiDIP-59257N.
STRINGi511145.b2288.

Structurei

3D structure databases

ProteinModelPortaliP0AFC3.
SMRiP0AFC3. Positions 15-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 3 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105KWU. Bacteria.
COG0838. LUCA.
HOGENOMiHOG000100121.
InParanoidiP0AFC3.
KOiK00330.
OMAiWVGFIEA.
PhylomeDBiP0AFC3.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA. 1 hit.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF
60 70 80 90 100
ESGIDSVGSA RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF
110 120 130 140
VEAAIFIFVL LAGLVYLVRI GALDWTPARS RRERMNPETN SIANRQR
Length:147
Mass (Da):16,457
Last modified:December 20, 2005 - v1
Checksum:i1F9E0804B2B0D1B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601A → G in CAA48360 (PubMed:7690854).Curated
Sequence conflicti82 – 9110ALYLFAWSTS → GAVSVRMVLL in CAA48360 (PubMed:7690854).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48360.1.
U00096 Genomic DNA. Translation: AAC75348.1.
AP009048 Genomic DNA. Translation: BAA16123.1.
PIRiF65000.
RefSeqiNP_416791.3. NC_000913.3.
WP_000062997.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75348; AAC75348; b2288.
BAA16123; BAA16123; BAA16123.
GeneIDi946764.
KEGGiecj:JW2283.
eco:b2288.
PATRICi32119943. VBIEscCol129921_2381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48360.1.
U00096 Genomic DNA. Translation: AAC75348.1.
AP009048 Genomic DNA. Translation: BAA16123.1.
PIRiF65000.
RefSeqiNP_416791.3. NC_000913.3.
WP_000062997.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFC3.
SMRiP0AFC3. Positions 15-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262974. 23 interactions.
DIPiDIP-59257N.
STRINGi511145.b2288.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AFC3.
PRIDEiP0AFC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75348; AAC75348; b2288.
BAA16123; BAA16123; BAA16123.
GeneIDi946764.
KEGGiecj:JW2283.
eco:b2288.
PATRICi32119943. VBIEscCol129921_2381.

Organism-specific databases

EchoBASEiEB2007.
EcoGeneiEG12082. nuoA.

Phylogenomic databases

eggNOGiENOG4105KWU. Bacteria.
COG0838. LUCA.
HOGENOMiHOG000100121.
InParanoidiP0AFC3.
KOiK00330.
OMAiWVGFIEA.
PhylomeDBiP0AFC3.

Enzyme and pathway databases

BioCyciEcoCyc:NUOA-MONOMER.
ECOL316407:JW2283-MONOMER.
MetaCyc:NUOA-MONOMER.

Miscellaneous databases

PROiP0AFC3.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA. 1 hit.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOA_ECOLI
AccessioniPrimary (citable) accession number: P0AFC3
Secondary accession number(s): P33597, P77159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.