Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogen regulation protein NR(I)

Gene

glnG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 1758ATPPROSITE-ProRule annotation
Nucleotide bindingi231 – 24010ATPPROSITE-ProRule annotation
DNA bindingi445 – 46420H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-NRI.
ECOL316407:JW3839-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulation protein NR(I)
Gene namesi
Name:glnG
Synonyms:glnT, ntrC
Ordered Locus Names:b3868, JW3839
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10385. glnG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Nitrogen regulation protein NR(I)PRO_0000081165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 5414-aspartylphosphatePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFB8.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
evgAP0ACZ43EBI-1113197,EBI-548694
glnLP0AFB53EBI-1113197,EBI-701156

Protein-protein interaction databases

BioGridi4262628. 31 interactions.
DIPiDIP-9781N.
IntActiP0AFB8. 6 interactions.
STRINGi511145.b3868.

Structurei

3D structure databases

ProteinModelPortaliP0AFB8.
SMRiP0AFB8. Positions 1-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 119115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini140 – 369230Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiP0AFB8.
KOiK07712.
OMAiGHSPVQV.
PhylomeDBiP0AFB8.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR010114. Sig_transdc_resp-reg_NtrC.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01818. ntrC. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGAEVL EALASKTPDV
60 70 80 90 100
LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD
110 120 130 140 150
YLPKPFDIDE AVALVERAIS HYQEQQQPRN VQLNGPTTDI IGEAPAMQDV
160 170 180 190 200
FRIIGRLSRS SISVLINGES GTGKELVAHA LHRHSPRAKA PFIALNMAAI
210 220 230 240 250
PKDLIESELF GHEKGAFTGA NTIRQGRFEQ ADGGTLFLDE IGDMPLDVQT
260 270 280 290 300
RLLRVLADGQ FYRVGGYAPV KVDVRIIAAT HQNLEQRVQE GKFREDLFHR
310 320 330 340 350
LNVIRVHLPP LRERREDIPR LARHFLQVAA RELGVEAKLL HPETEAALTR
360 370 380 390 400
LAWPGNVRQL ENTCRWLTVM AAGQEVLIQD LPGELFESTV AESTSQMQPD
410 420 430 440 450
SWATLLAQWA DRALRSGHQN LLSEAQPELE RTLLTTALRH TQGHKQEAAR
460
LLGWGRNTLT RKLKELGME
Length:469
Mass (Da):52,255
Last modified:December 20, 2005 - v1
Checksum:i645F401D4A10EFA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1443GEA → AK in CAA28808 (PubMed:2882477).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05173 Genomic DNA. Translation: CAA28808.1.
L19201 Genomic DNA. Translation: AAB03002.1.
U00096 Genomic DNA. Translation: AAC76865.1.
AP009048 Genomic DNA. Translation: BAE77441.1.
D16509 Genomic DNA. Translation: BAA03962.1.
PIRiB30377. RGECGG.
RefSeqiNP_418304.1. NC_000913.3.
WP_001188777.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76865; AAC76865; b3868.
BAE77441; BAE77441; BAE77441.
GeneIDi948361.
KEGGiecj:JW3839.
eco:b3868.
PATRICi32123239. VBIEscCol129921_3980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05173 Genomic DNA. Translation: CAA28808.1.
L19201 Genomic DNA. Translation: AAB03002.1.
U00096 Genomic DNA. Translation: AAC76865.1.
AP009048 Genomic DNA. Translation: BAE77441.1.
D16509 Genomic DNA. Translation: BAA03962.1.
PIRiB30377. RGECGG.
RefSeqiNP_418304.1. NC_000913.3.
WP_001188777.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFB8.
SMRiP0AFB8. Positions 1-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262628. 31 interactions.
DIPiDIP-9781N.
IntActiP0AFB8. 6 interactions.
STRINGi511145.b3868.

Proteomic databases

PaxDbiP0AFB8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76865; AAC76865; b3868.
BAE77441; BAE77441; BAE77441.
GeneIDi948361.
KEGGiecj:JW3839.
eco:b3868.
PATRICi32123239. VBIEscCol129921_3980.

Organism-specific databases

EchoBASEiEB0380.
EcoGeneiEG10385. glnG.

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiP0AFB8.
KOiK07712.
OMAiGHSPVQV.
PhylomeDBiP0AFB8.

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-NRI.
ECOL316407:JW3839-MONOMER.

Miscellaneous databases

PROiP0AFB8.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR010114. Sig_transdc_resp-reg_NtrC.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01818. ntrC. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTRC_ECOLI
AccessioniPrimary (citable) accession number: P0AFB8
Secondary accession number(s): P06713, Q2M8G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.