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Protein

Nitrogen regulation protein NR(I)

Gene

glnG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 175ATPPROSITE-ProRule annotation8
Nucleotide bindingi231 – 240ATPPROSITE-ProRule annotation10
DNA bindingi445 – 464H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processNitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-NRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulation protein NR(I)
Gene namesi
Name:glnG
Synonyms:glnT, ntrC
Ordered Locus Names:b3868, JW3839
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10385. glnG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811651 – 469Nitrogen regulation protein NR(I)Add BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFB8.
PRIDEiP0AFB8.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262628. 31 interactors.
DIPiDIP-9781N.
IntActiP0AFB8. 6 interactors.
STRINGi316385.ECDH10B_4058.

Structurei

3D structure databases

ProteinModelPortaliP0AFB8.
SMRiP0AFB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 119Response regulatoryPROSITE-ProRule annotationAdd BLAST115
Domaini140 – 369Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST230

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiP0AFB8.
KOiK07712.
PhylomeDBiP0AFB8.

Family and domain databases

CDDicd00156. REC. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeobox-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR010114. Sig_transdc_resp-reg_NtrC.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
PANTHERiPTHR32071:SF45. PTHR32071:SF45. 1 hit.
PfamiView protein in Pfam
PF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
PRINTSiPR01590. HTHFIS.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01818. ntrC. 1 hit.
PROSITEiView protein in PROSITE
PS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGAEVL EALASKTPDV
60 70 80 90 100
LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD
110 120 130 140 150
YLPKPFDIDE AVALVERAIS HYQEQQQPRN VQLNGPTTDI IGEAPAMQDV
160 170 180 190 200
FRIIGRLSRS SISVLINGES GTGKELVAHA LHRHSPRAKA PFIALNMAAI
210 220 230 240 250
PKDLIESELF GHEKGAFTGA NTIRQGRFEQ ADGGTLFLDE IGDMPLDVQT
260 270 280 290 300
RLLRVLADGQ FYRVGGYAPV KVDVRIIAAT HQNLEQRVQE GKFREDLFHR
310 320 330 340 350
LNVIRVHLPP LRERREDIPR LARHFLQVAA RELGVEAKLL HPETEAALTR
360 370 380 390 400
LAWPGNVRQL ENTCRWLTVM AAGQEVLIQD LPGELFESTV AESTSQMQPD
410 420 430 440 450
SWATLLAQWA DRALRSGHQN LLSEAQPELE RTLLTTALRH TQGHKQEAAR
460
LLGWGRNTLT RKLKELGME
Length:469
Mass (Da):52,255
Last modified:December 20, 2005 - v1
Checksum:i645F401D4A10EFA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142 – 144GEA → AK in CAA28808 (PubMed:2882477).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05173 Genomic DNA. Translation: CAA28808.1.
L19201 Genomic DNA. Translation: AAB03002.1.
U00096 Genomic DNA. Translation: AAC76865.1.
AP009048 Genomic DNA. Translation: BAE77441.1.
D16509 Genomic DNA. Translation: BAA03962.1.
PIRiB30377. RGECGG.
RefSeqiNP_418304.1. NC_000913.3.
WP_001188777.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76865; AAC76865; b3868.
BAE77441; BAE77441; BAE77441.
GeneIDi948361.
KEGGiecj:JW3839.
eco:b3868.
PATRICifig|1411691.4.peg.2843.

Similar proteinsi

Entry informationi

Entry nameiNTRC_ECOLI
AccessioniPrimary (citable) accession number: P0AFB8
Secondary accession number(s): P06713, Q2M8G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 20, 2005
Last modified: August 30, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene