Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogen regulation protein NR(II)

Gene

glnL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of NtrC. In nitrogen limitation NtrB activates NtrC by phosphorylating it, while in nitrogen excess NtrC is dephosphorylated and consequently inactivated by NtrB.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • nitrogen fixation Source: UniProtKB-KW
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • protein dephosphorylation Source: GOC
  • regulation of nitrogen compound metabolic process Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
  • signal transduction by protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nitrogen fixation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-NRII.
ECOL316407:JW3840-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulation protein NR(II) (EC:2.7.13.3)
Gene namesi
Name:glnL
Synonyms:glnR, ntrB
Ordered Locus Names:b3869, JW3840
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10387. glnL.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Nitrogen regulation protein NR(II)PRO_0000074820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFB5.

PTM databases

iPTMnetiP0AFB5.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-701156,EBI-701156
glnBP0A9Z15EBI-701156,EBI-551053
glnGP0AFB83EBI-701156,EBI-1113197
glnKP0AC555EBI-701156,EBI-559503
tfaEP091534EBI-701156,EBI-9133821

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262629. 6 interactions.
DIPiDIP-9784N.
IntActiP0AFB5. 10 interactions.
STRINGi511145.b3869.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi195 – 1973Combined sources
Helixi199 – 21012Combined sources
Turni211 – 2133Combined sources
Beta strandi218 – 2236Combined sources
Beta strandi231 – 2333Combined sources
Helixi235 – 25319Combined sources
Helixi254 – 2563Combined sources
Beta strandi258 – 27215Combined sources
Beta strandi275 – 28814Combined sources
Helixi314 – 32512Combined sources
Beta strandi329 – 3357Combined sources
Beta strandi338 – 34811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R62X-ray1.60A190-349[»]
ProteinModelPortaliP0AFB5.
SMRiP0AFB5. Positions 12-108, 137-349.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFB5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7874PASPROSITE-ProRule annotationAdd
BLAST
Domaini136 – 349214Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CUX. Bacteria.
COG3852. LUCA.
HOGENOMiHOG000262169.
InParanoidiP0AFB5.
KOiK07708.
OMAiEMRKIDQ.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AFB5.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGTQPDAG QILNSLINSI LLIDDNLAIH YANPAAQQLL AQSSRKLFGT
60 70 80 90 100
PLPELLSYFS LNIELMQESL EAGQGFTDNE VTLVIDGRSH ILSVTAQRMP
110 120 130 140 150
DGMILLEMAP MDNQRRLSQE QLQHAQQVAA RDLVRGLAHE IKNPLGGLRG
160 170 180 190 200
AAQLLSKALP DPSLLEYTKV IIEQADRLRN LVDRLLGPQL PGTRVTESIH
210 220 230 240 250
KVAERVVTLV SMELPDNVRL IRDYDPSLPE LAHDPDQIEQ VLLNIVRNAL
260 270 280 290 300
QALGPEGGEI ILRTRTAFQL TLHGERYRLA ARIDVEDNGP GIPPHLQDTL
310 320 330 340
FYPMVSGREG GTGLGLSIAR NLIDQHSGKI EFTSWPGHTE FSVYLPIRK
Length:349
Mass (Da):38,556
Last modified:January 1, 1988 - v1
Checksum:i8B6B3BB18B2E2E98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05173 Genomic DNA. Translation: CAA28807.1.
L19201 Genomic DNA. Translation: AAB03003.1.
U00096 Genomic DNA. Translation: AAC76866.1.
AP009048 Genomic DNA. Translation: BAE77440.1.
K02176 Genomic DNA. Translation: AAA23881.1.
PIRiA30377. RGECGL.
RefSeqiNP_418305.1. NC_000913.3.
WP_000190577.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76866; AAC76866; b3869.
BAE77440; BAE77440; BAE77440.
GeneIDi948360.
KEGGiecj:JW3840.
eco:b3869.
PATRICi32123241. VBIEscCol129921_3981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05173 Genomic DNA. Translation: CAA28807.1.
L19201 Genomic DNA. Translation: AAB03003.1.
U00096 Genomic DNA. Translation: AAC76866.1.
AP009048 Genomic DNA. Translation: BAE77440.1.
K02176 Genomic DNA. Translation: AAA23881.1.
PIRiA30377. RGECGL.
RefSeqiNP_418305.1. NC_000913.3.
WP_000190577.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R62X-ray1.60A190-349[»]
ProteinModelPortaliP0AFB5.
SMRiP0AFB5. Positions 12-108, 137-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262629. 6 interactions.
DIPiDIP-9784N.
IntActiP0AFB5. 10 interactions.
STRINGi511145.b3869.

PTM databases

iPTMnetiP0AFB5.

Proteomic databases

PaxDbiP0AFB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76866; AAC76866; b3869.
BAE77440; BAE77440; BAE77440.
GeneIDi948360.
KEGGiecj:JW3840.
eco:b3869.
PATRICi32123241. VBIEscCol129921_3981.

Organism-specific databases

EchoBASEiEB0382.
EcoGeneiEG10387. glnL.

Phylogenomic databases

eggNOGiENOG4105CUX. Bacteria.
COG3852. LUCA.
HOGENOMiHOG000262169.
InParanoidiP0AFB5.
KOiK07708.
OMAiEMRKIDQ.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AFB5.

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-NRII.
ECOL316407:JW3840-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFB5.
PROiP0AFB5.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete nucleotide sequence of the glnALG operon of Escherichia coli K12."
    Miranda-Rios J., Sanchez-Pescador R., Urdea M., Covarrubias A.A.
    Nucleic Acids Res. 15:2757-2770(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification and regulation of the glnL operator-promoter of the complex glnALG operon of Escherichia coli."
    Ueno-Nishio S., Mango S., Reitzer L.J., Magasanik B.
    J. Bacteriol. 160:379-384(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
  6. "Nucleotide sequence of the glnA-glnL intercistronic region of Escherichia coli."
    Rocha M., Vazquez M., Garciarrubio A., Covarrubias A.A.
    Gene 37:91-99(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
  7. "Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII."
    Ninfa A.J., Bennett R.L.
    J. Biol. Chem. 266:6888-6893(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT HIS-139.

Entry informationi

Entry nameiNTRB_ECOLI
AccessioniPrimary (citable) accession number: P0AFB5
Secondary accession number(s): P06712, Q2M8G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: February 17, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.