P0AFA9 (NIKC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nickel transport system permease protein NikC

Gene names

Name:	nikC
Ordered Locus Names:	b3478, JW3443

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane.
Subunit structure	Probably forms a heterodimeric pore with NikB.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Ion transport Nickel transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Nickel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nickel cation binding Inferred from direct assay PubMed 7867647. Source: EcoCyc nickel cation transmembrane transporter activity Inferred from mutant phenotype Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Nickel transport system permease protein NikC		PRO_0000060121
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Potential
	Topological domain	34 – 78	45	Periplasmic Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Topological domain	100 – 120	21	Cytoplasmic Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Topological domain	142 – 183	42	Periplasmic Potential
	Transmembrane	184 – 204	21	Helical; Potential
	Topological domain	205 – 207	3	Cytoplasmic Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Topological domain	229 – 239	11	Periplasmic Potential
	Transmembrane	240 – 260	21	Helical; Potential
	Topological domain	261 – 277	17	Cytoplasmic Potential
	Domain	67 – 260	194	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AFA9 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: F64769E44EEA1D6F	FASTA	277	30,362
	10 20 30 40 50 60 MNFFLSSRWS VRLALIIIAL LALIALTSQW WLPYDPQAID LPSRLLSPDA QHWLGTDHLG 70 80 90 100 110 120 RDIFSRLMAA TRVSLGSVMA CLLLVLTLGL VIGGSAGLIG GRVDQATMRV ADMFMTFPTS 130 140 150 160 170 180 ILSFFMVGVL GTGLTNVIIA IALSHWAWYA RMVRSLVISL RQREFVLASR LSGAGHVRVF 190 200 210 220 230 240 VDHLAGAVIP SLLVLATLDI GHMMLHVAGM SFLGLGVTAP TAEWGVMIND ARQYIWTQPL 250 260 270 QMFWPGLALF ISVMAFNLVG DALRDHLDPH LVTEHAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel." Navarro C., Wu L.-F., Mandrand-Berthelot M.-A. Mol. Microbiol. 9:1181-1191(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73143 Genomic DNA. Translation: CAA51661.1. U00039 Genomic DNA. Translation: AAB18453.1. U00096 Genomic DNA. Translation: AAC76503.1. AP009048 Genomic DNA. Translation: BAE77815.1.
PIR	S39596.
RefSeq	NP_417935.1. NC_000913.2. YP_491956.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFA9.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48065N.
STRING	511145.b3478.
Protein family/group databases
TCDB	3.A.1.5.3. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PaxDb	P0AFA9.
PRIDE	P0AFA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76503; AAC76503; b3478. BAE77815; BAE77815; BAE77815.
GeneID	12934261. 947990.
KEGG	ecj:Y75_p3700. eco:b3478.
PATRIC	32122400. VBIEscCol129921_3577.
Organism-specific databases
EchoBASE	EB2002.
EcoGene	EG12077. nikC.
Phylogenomic databases
eggNOG	COG1173.
HOGENOM	HOG000171368.
KO	K15586.
OMA	GAVMACL.
ProtClustDB	PRK10417.
Enzyme and pathway databases
BioCyc	EcoCyc:NIKC-MONOMER. ECOL316407:JW3443-MONOMER.
Gene expression databases
Genevestigator	P0AFA9.
Family and domain databases
InterPro	IPR000515. BPD_transp. IPR014157. Nickel_NikC. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02790. nickel_nikC. 1 hit.
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIKC_ECOLI

Accession

Primary (citable) accession number: P0AFA9
Secondary accession number(s): P33592, Q2M7E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program