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Protein

Nickel transport system permease protein NikC

Gene

nikC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane.

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • nickel cation transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • nickel cation transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Nickel transport, Transport

Keywords - Ligandi

Nickel

Enzyme and pathway databases

BioCyciEcoCyc:NIKC-MONOMER.
ECOL316407:JW3443-MONOMER.
MetaCyc:NIKC-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel transport system permease protein NikC
Gene namesi
Name:nikC
Ordered Locus Names:b3478, JW3443
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12077. nikC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini34 – 7845PeriplasmicSequence analysisAdd
BLAST
Transmembranei79 – 9921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini100 – 12021CytoplasmicSequence analysisAdd
BLAST
Transmembranei121 – 14121HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini142 – 18342PeriplasmicSequence analysisAdd
BLAST
Transmembranei184 – 20421HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini205 – 2073CytoplasmicSequence analysis
Transmembranei208 – 22821HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini229 – 23911PeriplasmicSequence analysisAdd
BLAST
Transmembranei240 – 26021HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini261 – 27717CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Nickel transport system permease protein NikCPRO_0000060121Add
BLAST

Proteomic databases

PaxDbiP0AFA9.
PRIDEiP0AFA9.

Interactioni

Subunit structurei

Probably forms a heterodimeric pore with NikB.

Protein-protein interaction databases

BioGridi4263255. 7 interactions.
DIPiDIP-48065N.
STRINGi511145.b3478.

Structurei

3D structure databases

ProteinModelPortaliP0AFA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 260194ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2T. Bacteria.
COG1173. LUCA.
HOGENOMiHOG000171368.
KOiK15586.
OMAiLMTATRV.
OrthoDBiEOG6T7N3V.
PhylomeDBiP0AFA9.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
IPR014157. Nickel_NikC.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR02790. nickel_nikC. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFFLSSRWS VRLALIIIAL LALIALTSQW WLPYDPQAID LPSRLLSPDA
60 70 80 90 100
QHWLGTDHLG RDIFSRLMAA TRVSLGSVMA CLLLVLTLGL VIGGSAGLIG
110 120 130 140 150
GRVDQATMRV ADMFMTFPTS ILSFFMVGVL GTGLTNVIIA IALSHWAWYA
160 170 180 190 200
RMVRSLVISL RQREFVLASR LSGAGHVRVF VDHLAGAVIP SLLVLATLDI
210 220 230 240 250
GHMMLHVAGM SFLGLGVTAP TAEWGVMIND ARQYIWTQPL QMFWPGLALF
260 270
ISVMAFNLVG DALRDHLDPH LVTEHAH
Length:277
Mass (Da):30,362
Last modified:December 20, 2005 - v1
Checksum:iF64769E44EEA1D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51661.1.
U00039 Genomic DNA. Translation: AAB18453.1.
U00096 Genomic DNA. Translation: AAC76503.1.
AP009048 Genomic DNA. Translation: BAE77815.1.
PIRiS39596.
RefSeqiNP_417935.1. NC_000913.3.
WP_001008963.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76503; AAC76503; b3478.
BAE77815; BAE77815; BAE77815.
GeneIDi947990.
KEGGiecj:JW3443.
eco:b3478.
PATRICi32122400. VBIEscCol129921_3577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51661.1.
U00039 Genomic DNA. Translation: AAB18453.1.
U00096 Genomic DNA. Translation: AAC76503.1.
AP009048 Genomic DNA. Translation: BAE77815.1.
PIRiS39596.
RefSeqiNP_417935.1. NC_000913.3.
WP_001008963.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263255. 7 interactions.
DIPiDIP-48065N.
STRINGi511145.b3478.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFA9.
PRIDEiP0AFA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76503; AAC76503; b3478.
BAE77815; BAE77815; BAE77815.
GeneIDi947990.
KEGGiecj:JW3443.
eco:b3478.
PATRICi32122400. VBIEscCol129921_3577.

Organism-specific databases

EchoBASEiEB2002.
EcoGeneiEG12077. nikC.

Phylogenomic databases

eggNOGiENOG4105C2T. Bacteria.
COG1173. LUCA.
HOGENOMiHOG000171368.
KOiK15586.
OMAiLMTATRV.
OrthoDBiEOG6T7N3V.
PhylomeDBiP0AFA9.

Enzyme and pathway databases

BioCyciEcoCyc:NIKC-MONOMER.
ECOL316407:JW3443-MONOMER.
MetaCyc:NIKC-MONOMER.

Miscellaneous databases

PROiP0AFA9.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
IPR014157. Nickel_NikC.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR02790. nickel_nikC. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel."
    Navarro C., Wu L.-F., Mandrand-Berthelot M.-A.
    Mol. Microbiol. 9:1181-1191(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNIKC_ECOLI
AccessioniPrimary (citable) accession number: P0AFA9
Secondary accession number(s): P33592, Q2M7E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.