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Protein

Na(+)/H(+) antiporter NhaB

Gene

nhaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 3 H+ per 2 Na+. Has a high affinity for sodium, but can also transport lithium. Activity is weakly pH-dependent. Essential for regulation of intracellular pH under alkaline conditions.4 Publications

Kineticsi

  1. KM=16.66 mM for Na+ (at pH 7.2)1 Publication
  2. KM=3.44 mM for Na+ (at pH 7.6)1 Publication
  3. KM=1.53 mM for Na+ (at pH 8.5)1 Publication
  1. Vmax=107 µmol/min/mg enzyme (at pH 7.2)1 Publication
  2. Vmax=67.5 µmol/min/mg enzyme (at pH 7.6)1 Publication
  3. Vmax=87.8 µmol/min/mg enzyme (at pH 8.5)1 Publication

GO - Molecular functioni

  • cation:cation antiporter activity Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoliWiki

GO - Biological processi

  • anion transmembrane transport Source: GO_Central
  • intracellular pH reduction Source: EcoCyc
  • proton transport Source: EcoCyc
  • response to lithium ion Source: EcoliWiki
  • sodium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:NHAB-MONOMER.
ECOL316407:JW1175-MONOMER.
MetaCyc:NHAB-MONOMER.

Protein family/group databases

TCDBi2.A.34.1.1. the nhab na(+):h(+) antiporter (nhab) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) antiporter NhaB
Alternative name(s):
Sodium/proton antiporter NhaB
Gene namesi
Name:nhaB
Ordered Locus Names:b1186, JW1175
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11392. nhaB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence analysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence analysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence analysisAdd
BLAST
Transmembranei348 – 36821HelicalSequence analysisAdd
BLAST
Transmembranei391 – 41121HelicalSequence analysisAdd
BLAST
Transmembranei447 – 46721HelicalSequence analysisAdd
BLAST
Transmembranei475 – 49521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Na(+)/H(+) antiporter NhaBPRO_0000052412Add
BLAST

Proteomic databases

PaxDbiP0AFA7.

Interactioni

Protein-protein interaction databases

BioGridi4260101. 13 interactions.
STRINGi511145.b1186.

Structurei

3D structure databases

ProteinModelPortaliP0AFA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DKR. Bacteria.
COG3067. LUCA.
HOGENOMiHOG000218133.
InParanoidiP0AFA7.
KOiK03314.
OMAiQFEMLAV.
OrthoDBiEOG6XDGRV.

Family and domain databases

HAMAPiMF_01599. NhaB.
InterProiIPR004671. Na+/H+_antiporter_NhaB.
[Graphical view]
PfamiPF06450. NhaB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00774. NhaB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISWGRALW RNFLGQSPDW YKLALIIFLI VNPLIFLISP FVAGWLLVAE
60 70 80 90 100
FIFTLAMALK CYPLLPGGLL AIEAVFIGMT SAEHVREEVA ANLEVLLLLM
110 120 130 140 150
FMVAGIYFMK QLLLFIFTRL LLSIRSKMLL SLSFCVAAAF LSAFLDALTV
160 170 180 190 200
VAVVISVAVG FYGIYHRVAS SRTEDTDLQD DSHIDKHYKV VLEQFRGFLR
210 220 230 240 250
SLMMHAGVGT ALGGVMTMVG EPQNLIIAKA AGWHFGDFFL RMSPVTVPVL
260 270 280 290 300
ICGLLTCLLV EKLRWFGYGE TLPEKVREVL QQFDDQSRHQ RTRQDKIRLI
310 320 330 340 350
VQAIIGVWLV TALALHLAEV GLIGLSVIIL ATSLTGVTDE HAIGKAFTES
360 370 380 390 400
LPFTALLTVF FSVVAVIIDQ QLFSPIIQFV LQASEHAQLS LFYIFNGLLS
410 420 430 440 450
SISDNVFVGT IYINEAKAAM ESGAITLKQY ELLAVAINTG TNLPSVATPN
460 470 480 490 500
GQAAFLFLLT SALAPLIRLS YGRMVWMALP YTLVLTLVGL LCVEFTLAPV
510
TEWFMQMGWI ATL
Length:513
Mass (Da):56,728
Last modified:December 20, 2005 - v1
Checksum:iB5D85BD8E761E2BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1111MEISWGRALWR → MA in AAA24218 (PubMed:1317851).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83655 Genomic DNA. Translation: AAA24218.1.
U00096 Genomic DNA. Translation: AAC74270.1.
AP009048 Genomic DNA. Translation: BAA36033.1.
PIRiG64864.
RefSeqiNP_415704.1. NC_000913.3.
WP_000406391.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74270; AAC74270; b1186.
BAA36033; BAA36033; BAA36033.
GeneIDi944822.
KEGGiecj:JW1175.
eco:b1186.
PATRICi32117618. VBIEscCol129921_1231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83655 Genomic DNA. Translation: AAA24218.1.
U00096 Genomic DNA. Translation: AAC74270.1.
AP009048 Genomic DNA. Translation: BAA36033.1.
PIRiG64864.
RefSeqiNP_415704.1. NC_000913.3.
WP_000406391.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260101. 13 interactions.
STRINGi511145.b1186.

Protein family/group databases

TCDBi2.A.34.1.1. the nhab na(+):h(+) antiporter (nhab) family.

Proteomic databases

PaxDbiP0AFA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74270; AAC74270; b1186.
BAA36033; BAA36033; BAA36033.
GeneIDi944822.
KEGGiecj:JW1175.
eco:b1186.
PATRICi32117618. VBIEscCol129921_1231.

Organism-specific databases

EchoBASEiEB1365.
EcoGeneiEG11392. nhaB.

Phylogenomic databases

eggNOGiENOG4105DKR. Bacteria.
COG3067. LUCA.
HOGENOMiHOG000218133.
InParanoidiP0AFA7.
KOiK03314.
OMAiQFEMLAV.
OrthoDBiEOG6XDGRV.

Enzyme and pathway databases

BioCyciEcoCyc:NHAB-MONOMER.
ECOL316407:JW1175-MONOMER.
MetaCyc:NHAB-MONOMER.

Miscellaneous databases

PROiP0AFA7.

Family and domain databases

HAMAPiMF_01599. NhaB.
InterProiIPR004671. Na+/H+_antiporter_NhaB.
[Graphical view]
PfamiPF06450. NhaB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00774. NhaB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli."
    Pinner E., Padan E., Schuldiner S.
    J. Biol. Chem. 267:11064-11068(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli."
    Pinner E., Kotler Y., Padan E., Schuldiner S.
    J. Biol. Chem. 268:1729-1734(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Lithium toxicity and Na+(Li+)/H+ antiporter in Escherichia coli."
    Inaba K., Kuroda T., Shimamoto T., Kayahara T., Tsuda M., Tsuchiya T.
    Biol. Pharm. Bull. 17:395-398(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in Escherichia coli."
    Shimamoto T., Inaba K., Thelen P., Ishikawa T., Goldberg E.B., Tsuda M., Tsuchiya T.
    J. Biochem. 116:285-290(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN INTRACELLULAR PH REGULATION.
    Strain: K12.
  8. "Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli."
    Pinner E., Padan E., Schuldiner S.
    J. Biol. Chem. 269:26274-26279(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, STOICHIOMETRY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K12.
  9. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNHAB_ECOLI
AccessioniPrimary (citable) accession number: P0AFA7
Secondary accession number(s): P27377, P77533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.