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Protein

Na(+)/H(+) antiporter NhaB

Gene

nhaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 3 H+ per 2 Na+. Has a high affinity for sodium, but can also transport lithium. Activity is weakly pH-dependent. Essential for regulation of intracellular pH under alkaline conditions.4 Publications

Kineticsi

  1. KM=16.66 mM for Na+ (at pH 7.2)1 Publication
  2. KM=3.44 mM for Na+ (at pH 7.6)1 Publication
  3. KM=1.53 mM for Na+ (at pH 8.5)1 Publication
  1. Vmax=107 µmol/min/mg enzyme (at pH 7.2)1 Publication
  2. Vmax=67.5 µmol/min/mg enzyme (at pH 7.6)1 Publication
  3. Vmax=87.8 µmol/min/mg enzyme (at pH 8.5)1 Publication

GO - Molecular functioni

  • cation:cation antiporter activity Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoliWiki

GO - Biological processi

  • intracellular pH reduction Source: EcoCyc
  • response to lithium ion Source: EcoliWiki
  • sodium ion transport Source: EcoCyc

Keywordsi

Biological processAntiport, Ion transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

BioCyciEcoCyc:NHAB-MONOMER
MetaCyc:NHAB-MONOMER

Protein family/group databases

TCDBi2.A.34.1.1 the nhab na(+):h(+) antiporter (nhab) family

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) antiporter NhaB
Alternative name(s):
Sodium/proton antiporter NhaB
Gene namesi
Name:nhaB
Ordered Locus Names:b1186, JW1175
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG11392 nhaB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
Transmembranei475 – 495HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000524121 – 513Na(+)/H(+) antiporter NhaBAdd BLAST513

Proteomic databases

PaxDbiP0AFA7
PRIDEiP0AFA7

Interactioni

Protein-protein interaction databases

BioGridi4260101, 18 interactors
STRINGi316385.ECDH10B_1239

Structurei

3D structure databases

ProteinModelPortaliP0AFA7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DKR Bacteria
COG3067 LUCA
HOGENOMiHOG000218133
InParanoidiP0AFA7
KOiK03314
OMAiQFEMLAV

Family and domain databases

HAMAPiMF_01599 NhaB, 1 hit
InterProiView protein in InterPro
IPR004671 Na+/H+_antiporter_NhaB
PANTHERiPTHR43302:SF1 PTHR43302:SF1, 1 hit
PfamiView protein in Pfam
PF06450 NhaB, 1 hit
TIGRFAMsiTIGR00774 NhaB, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISWGRALW RNFLGQSPDW YKLALIIFLI VNPLIFLISP FVAGWLLVAE
60 70 80 90 100
FIFTLAMALK CYPLLPGGLL AIEAVFIGMT SAEHVREEVA ANLEVLLLLM
110 120 130 140 150
FMVAGIYFMK QLLLFIFTRL LLSIRSKMLL SLSFCVAAAF LSAFLDALTV
160 170 180 190 200
VAVVISVAVG FYGIYHRVAS SRTEDTDLQD DSHIDKHYKV VLEQFRGFLR
210 220 230 240 250
SLMMHAGVGT ALGGVMTMVG EPQNLIIAKA AGWHFGDFFL RMSPVTVPVL
260 270 280 290 300
ICGLLTCLLV EKLRWFGYGE TLPEKVREVL QQFDDQSRHQ RTRQDKIRLI
310 320 330 340 350
VQAIIGVWLV TALALHLAEV GLIGLSVIIL ATSLTGVTDE HAIGKAFTES
360 370 380 390 400
LPFTALLTVF FSVVAVIIDQ QLFSPIIQFV LQASEHAQLS LFYIFNGLLS
410 420 430 440 450
SISDNVFVGT IYINEAKAAM ESGAITLKQY ELLAVAINTG TNLPSVATPN
460 470 480 490 500
GQAAFLFLLT SALAPLIRLS YGRMVWMALP YTLVLTLVGL LCVEFTLAPV
510
TEWFMQMGWI ATL
Length:513
Mass (Da):56,728
Last modified:December 20, 2005 - v1
Checksum:iB5D85BD8E761E2BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 11MEISWGRALWR → MA in AAA24218 (PubMed:1317851).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83655 Genomic DNA Translation: AAA24218.1
U00096 Genomic DNA Translation: AAC74270.1
AP009048 Genomic DNA Translation: BAA36033.1
PIRiG64864
RefSeqiNP_415704.1, NC_000913.3
WP_000406391.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74270; AAC74270; b1186
BAA36033; BAA36033; BAA36033
GeneIDi944822
KEGGiecj:JW1175
eco:b1186
PATRICifig|1411691.4.peg.1101

Entry informationi

Entry nameiNHAB_ECOLI
AccessioniPrimary (citable) accession number: P0AFA7
Secondary accession number(s): P27377, P77533
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 25, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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