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Protein

Bacteriophage N4 adsorption protein B

Gene

nfrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Required for bacteriophage N4 adsorption. May be a component of the phage receptor.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11739-MONOMER.
ECOL316407:JW0558-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacteriophage N4 adsorption protein B
Gene namesi
Name:nfrB
Ordered Locus Names:b0569, JW0558
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11739. nfrB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Transmembranei362 – 38221HelicalSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745Bacteriophage N4 adsorption protein BPRO_0000096796Add
BLAST

Proteomic databases

PaxDbiP0AFA5.
PRIDEiP0AFA5.

Interactioni

Protein-protein interaction databases

BioGridi4262937. 95 interactions.
DIPiDIP-10333N.
IntActiP0AFA5. 2 interactions.
MINTiMINT-1320212.
STRINGi511145.b0569.

Structurei

3D structure databases

ProteinModelPortaliP0AFA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410802X. Bacteria.
ENOG410YBKS. LUCA.
HOGENOMiHOG000117834.
KOiK11740.
OMAiKTMHDFP.
OrthoDBiEOG6KHFV6.

Family and domain databases

InterProiIPR001173. Glyco_trans_2-like.
IPR007831. GSPII_E_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13632. Glyco_trans_2_3. 1 hit.
PF05157. T2SSE_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWLLDVFAT WLYGLKVIAI TLAVIMFISG LDDFFIDVVY WVRRIKRKLS
60 70 80 90 100
VYRRYPRMSY RELYKPDEKP LAIMVPAWNE TGVIGNMAEL AATTLDYENY
110 120 130 140 150
HIFVGTYPND PDTQRDVDEV CARFPNVHKV VCARPGPTSK ADCLNNVLDA
160 170 180 190 200
ITQFERSANF AFAGFILHDA EDVISPMELR LFNYLVERKD LIQIPVYPFE
210 220 230 240 250
REWTHFTSMT YIDEFSELHG KDVPVREALA GQVPSAGVGT CFSRRAVTAL
260 270 280 290 300
LADGDGIAFD VQSLTEDYDI GFRLKEKGMT EIFVRFPVVD EAKEREQRKF
310 320 330 340 350
LQHARTSNMI CVREYFPDTF STAVRQKSRW IIGIVFQGFK THKWTSSLTL
360 370 380 390 400
NYFLWRDRKG AISNFVSFLA MLVMIQLLLL LAYESLWPDA WHFLSIFSGS
410 420 430 440 450
AWLMTLLWLN FGLMVNRIVQ RVIFVTGYYG LTQGLLSVLR LFWGNLINFM
460 470 480 490 500
ANWRALKQVL QHGDPRRVAW DKTTHDFPSV TGDTRSLRPL GQILLENQVI
510 520 530 540 550
TEEQLDTALR NRVEGLRLGG SMLMQGLISA EQLAQALAEQ NGVAWESIDA
560 570 580 590 600
WQIPSSLIAE MPASVALHYA VLPLRLENDE LIVGSEDGID PVSLAALTRK
610 620 630 640 650
VGRKVRYVIV LRGQIVTGLR HWYARRRGHD PRAMLYNAVQ HQWLTEQQAG
660 670 680 690 700
EIWRQYVPHQ FLFAEILTTL GHINRSAINV LLLRHERSSL PLGKFLVTEG
710 720 730 740
VISQETLDRV LTIQRELQVS MQSLLLKAGL NTEQVAQLES ENEGE
Length:745
Mass (Da):85,319
Last modified:December 20, 2005 - v1
Checksum:iB596A5D5B64654C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16945 Unassigned DNA. Translation: AAC36848.1.
U82598 Genomic DNA. Translation: AAB40767.1.
U00096 Genomic DNA. Translation: AAC73670.1.
AP009048 Genomic DNA. Translation: BAA35203.1.
PIRiA49351.
RefSeqiNP_415101.1. NC_000913.3.
WP_000383932.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73670; AAC73670; b0569.
BAA35203; BAA35203; BAA35203.
GeneIDi945849.
KEGGiecj:JW0558.
eco:b0569.
PATRICi32116306. VBIEscCol129921_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16945 Unassigned DNA. Translation: AAC36848.1.
U82598 Genomic DNA. Translation: AAB40767.1.
U00096 Genomic DNA. Translation: AAC73670.1.
AP009048 Genomic DNA. Translation: BAA35203.1.
PIRiA49351.
RefSeqiNP_415101.1. NC_000913.3.
WP_000383932.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262937. 95 interactions.
DIPiDIP-10333N.
IntActiP0AFA5. 2 interactions.
MINTiMINT-1320212.
STRINGi511145.b0569.

Proteomic databases

PaxDbiP0AFA5.
PRIDEiP0AFA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73670; AAC73670; b0569.
BAA35203; BAA35203; BAA35203.
GeneIDi945849.
KEGGiecj:JW0558.
eco:b0569.
PATRICi32116306. VBIEscCol129921_0593.

Organism-specific databases

EchoBASEiEB1690.
EcoGeneiEG11739. nfrB.

Phylogenomic databases

eggNOGiENOG410802X. Bacteria.
ENOG410YBKS. LUCA.
HOGENOMiHOG000117834.
KOiK11740.
OMAiKTMHDFP.
OrthoDBiEOG6KHFV6.

Enzyme and pathway databases

BioCyciEcoCyc:EG11739-MONOMER.
ECOL316407:JW0558-MONOMER.

Miscellaneous databases

PROiP0AFA5.

Family and domain databases

InterProiIPR001173. Glyco_trans_2-like.
IPR007831. GSPII_E_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13632. Glyco_trans_2_3. 1 hit.
PF05157. T2SSE_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption."
    Kiino D.R., Singer M.S., Rothman-Denes L.B.
    J. Bacteriol. 175:7081-7085(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNFRB_ECOLI
AccessioniPrimary (citable) accession number: P0AFA5
Secondary accession number(s): P31599
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 20, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.