Nitrate/nitrite sensor protein NarX - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0AFA2 (NARX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nitrate/nitrite sensor protein NarX
EC=2.7.13.3

Gene names

Name:	narX
Synonyms:	narR
Ordered Locus Names:	b1222, JW1213

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.8.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Nitrate assimilation Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to nitrate Inferred from expression pattern PubMed 2832370. Source: EcoCyc cellular response to nitrite Inferred from mutant phenotype PubMed 9426129. Source: EcoCyc nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW protein autophosphorylation Inferred from direct assay PubMed 8473280. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 1197071. Source: EcoliWiki
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from direct assay PubMed 8473280. Source: EcoCyc phosphorelay sensor kinase activity Inferred from direct assay PubMed 8473280. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 598

598

Nitrate/nitrite sensor protein NarX

PRO_0000074812

Regions

Topological domain

1 – 14

Cytoplasmic Potential

Transmembrane

15 – 37

Helical; Potential

Topological domain

38 – 151

114

Periplasmic Potential

Transmembrane

152 – 174

Helical; Potential

Topological domain

175 – 598

424

Cytoplasmic Potential

Domain

176 – 228

HAMP

Domain

393 – 587

195

Histidine kinase

Amino acid modifications

Modified residue

399

Phosphohistidine; by autocatalysis By similarity

Experimental info

Sequence conflict

42 – 52

GSAHAINKAGS → AAPMRSTKRDA Ref.1

Sequence conflict

42 – 52

GSAHAINKAGS → AAPMRSTKRDA Ref.5

Sequence conflict

374

A → G in AAA24198. Ref.6

Secondary structure

598

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFA2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 886BA0FC2C8F3C3E
Show »

FASTA

598

67,084

        10         20         30         40         50         60 
MLKRCLSPLT LVNQVALIVL LSTAIGLAGM AVSGWLVQGV QGSAHAINKA GSLRMQSYRL 

        70         80         90        100        110        120 
LAAVPLSEKD KPLIKEMEQT AFSAELTRAA ERDGQLAQLQ GLQDYWRNEL IPALMRAQNR 

       130        140        150        160        170        180 
ETVSADVSQF VAGLDQLVSG FDRTTEMRIE TVVLVHRVMA VFMALLLVFT IIWLRARLLQ 

       190        200        210        220        230        240 
PWRQLLAMAS AVSHRDFTQR ANISGRNEMA MLGTALNNMS AELAESYAVL EQRVQEKTAG 

       250        260        270        280        290        300 
LEHKNQILSF LWQANRRLHS RAPLCERLSP VLNGLQNLTL LRDIELRVYD TDDEENHQEF 

       310        320        330        340        350        360 
TCQPDMTCDD KGCQLCPRGV LPVGDRGTTL KWRLADSHTQ YGILLATLPQ GRHLSHDQQQ 

       370        380        390        400        410        420 
LVDTLVEQLT ATLALDRHQE RQQQLIVMEE RATIARELHD SIAQSLSCMK MQVSCLQMQG 

       430        440        450        460        470        480 
DALPESSREL LSQIRNELNA SWAQLRELLT TFRLQLTEPG LRPALEASCE EYSAKFGFPV 

       490        500        510        520        530        540 
KLDYQLPPRL VPSHQAIHLL QIAREALSNA LKHSQASEVV VTVAQNDNQV KLTVQDNGCG 

       550        560        570        580        590 
VPENAIRSNH YGMIIMRDRA QSLRGDCRVR RRESGGTEVV VTFIPEKTFT DVQGDTHE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes." Nohno T., Noji S., Taniguchi S., Saito T. Nucleic Acids Res. 17:2947-2957(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The narK gene product participates in nitrate transport induced in Escherichia coli nitrate-respiring cells." Noji S., Nohno T., Saito T., Taniguchi S. FEBS Lett. 252:139-143(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55. Strain: K12.
[6]	"Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12." Stewart V., Parales J. Jr., Merkel S.M. J. Bacteriol. 171:2229-2234(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 56-598. Strain: K12.
[7]	Cavicchioli R., Gunsalus R.P., Chiang R.C. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X13360 Genomic DNA. Translation: CAA31741.1.
U00096 Genomic DNA. Translation: AAC74306.1.
AP009048 Genomic DNA. Translation: BAA36090.1.
X15996 Genomic DNA. Translation: CAA34125.1.
M24910 Genomic DNA. Translation: AAA24198.1.
X65715 Genomic DNA. Translation: CAA46631.1.
X69189 Genomic DNA. Translation: CAA48934.1.

PIR

RGECNX. S26137.

RefSeq

NP_415740.1. NC_000913.2.
YP_489492.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EZH	X-ray	1.70	A/B	38-151	[»]
3EZI	X-ray	1.70	A/B/C/D	42-148	[»]

ProteinModelPortal

P0AFA2.

SMR

P0AFA2. Positions 42-151, 380-569.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35785N.

IntAct

P0AFA2. 4 interactions.

STRING

511145.b1222.

Proteomic databases

PaxDb

P0AFA2.

PRIDE

P0AFA2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74306; AAC74306; b1222.
BAA36090; BAA36090; BAA36090.

GeneID

12934276.
945788.

KEGG

ecj:Y75_p1197.
eco:b1222.

PATRIC

32117702. VBIEscCol129921_1273.

Organism-specific databases

EchoBASE

EB0640.

EcoGene

EG10646. narX.

Phylogenomic databases

eggNOG

COG3850.

HOGENOM

HOG000275465.

K07673.

OMA

LMLMEER.

ProtClustDB

PRK10600.

Enzyme and pathway databases

BioCyc

EcoCyc:NARX-MONOMER.
ECOL316407:JW1213-MONOMER.

Gene expression databases

Genevestigator

P0AFA2.

Family and domain databases

Gene3D

3.30.565.10. 1 hit.

InterPro

IPR003660. HAMP_linker_domain.
IPR003594. HATPase_ATP-bd.
IPR016380. Sig_transdc_His_kin_NarX/NarQ.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]

Pfam

PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]

PIRSF

PIRSF003167. STHK_NarX/NarQ. 1 hit.

SMART

SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.

PROSITE

PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0AFA2.

Entry information

Entry name

NARX_ECOLI

Accession

Primary (citable) accession number: P0AFA2
Secondary accession number(s): P10956

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents