P0AF98 (LPTF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide export system permease protein LptF

Gene names

Name:	lptF
Synonyms:	yjgP
Ordered Locus Names:	b4261, JW4218

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Ref.5
Subunit structure	The complex is probably composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the LptF/LptG family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide transport Inferred from mutant phenotype Ref.5. Source: EcoCyc
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from direct assay PubMed 19500581. Source: EcoCyc integral to membrane Inferred from direct assay Ref.4. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipopolysaccharide-transporting ATPase activity Inferred from direct assay PubMed 19500581. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Lipopolysaccharide export system permease protein LptF		PRO_0000169771
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Helical; Potential
	Topological domain	37 – 53	17	Periplasmic Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Topological domain	75 – 100	26	Cytoplasmic Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Topological domain	122 – 269	148	Periplasmic Potential
	Transmembrane	270 – 290	21	Helical; Potential
	Topological domain	291 – 295	5	Cytoplasmic Potential
	Transmembrane	296 – 316	21	Helical; Potential
	Topological domain	317 – 327	11	Periplasmic Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Topological domain	349 – 366	18	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AF98 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 69A564AA22CBFFEA	FASTA	366	40,358
	10 20 30 40 50 60 MIIIRYLVRE TLKSQLAILF ILLLIFFCQK LVRILGAAVD GDIPANLVLS LLGLGVPEMA 70 80 90 100 110 120 QLILPLSLFL GLLMTLGKLY TESEITVMHA CGLSKAVLVK AAMILAVFTA IVAAVNVMWA 130 140 150 160 170 180 GPWSSRHQDE VLAEAKANPG MAALAQGQFQ QATNGSSVLF IESVDGSDFK DVFLAQIRPK 190 200 210 220 230 240 GNARPSVVVA DSGHLTQLRD GSQVVTLNQG TRFEGTALLR DFRITDFQDY QAIIGHQAVA 250 260 270 280 290 300 LDPNDTDQMD MRTLWNTDTD RARAELNWRI TLVFTVFMMA LMVVPLSVVN PRQGRVLSML 310 320 330 340 350 360 PAMLLYLLFF LIQTSLKSNG GKGKLDPTLW MWTVNLIYLA LAIVLNLWDT VPVRRLRASF SRKGAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli." Ruiz N., Gronenberg L.S., Kahne D., Silhavy T.J. Proc. Natl. Acad. Sci. U.S.A. 105:5537-5542(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14003 Genomic DNA. Translation: AAA97158.1. U00096 Genomic DNA. Translation: AAC77218.1. AP009048 Genomic DNA. Translation: BAE78258.1.
PIR	S56487.
RefSeq	NP_418682.1. NC_000913.2. YP_492399.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AF98.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-7137013.
STRING	511145.b4261.
Protein family/group databases
TCDB	9.B.93.1.1. spanin family.
Protocols and materials databases
DNASU	948795.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77218; AAC77218; b4261. BAE78258; BAE78258; BAE78258.
GeneID	12933733. 948795.
KEGG	ecj:Y75_p4144. eco:b4261.
PATRIC	32124095. VBIEscCol129921_4392.
Organism-specific databases
EchoBASE	EB2424.
EcoGene	EG12535. lptF.
Phylogenomic databases
eggNOG	COG0795.
HOGENOM	HOG000283246.
KO	K07091.
OMA	WLTPWAI.
ProtClustDB	PRK15120.
Enzyme and pathway databases
BioCyc	EcoCyc:G7888-MONOMER. ECOL316407:JW4218-MONOMER.
Gene expression databases
Genevestigator	P0AF98.
Family and domain databases
InterPro	IPR005495. YjgP_YjgQ_permease_prd. [Graphical view]
Pfam	PF03739. YjgP_YjgQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPTF_ECOLI

Accession

Primary (citable) accession number: P0AF98
Secondary accession number(s): P39340, Q2M648

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program