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Protein

Regulator of ribonuclease activity B

Gene

rraB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome.UniRule annotation2 Publications

GO - Molecular functioni

  • endoribonuclease inhibitor activity Source: UniProtKB
  • enzyme binding Source: EcoCyc
  • ribonuclease inhibitor activity Source: EcoCyc

GO - Biological processi

  • mRNA catabolic process Source: EcoCyc
  • regulation of catalytic activity Source: EcoCyc
  • regulation of endoribonuclease activity Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7885-MONOMER.
ECOL316407:JW4212-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of ribonuclease activity BUniRule annotation
Gene namesi
Name:rraBUniRule annotation
Synonyms:yjgD
Ordered Locus Names:b4255, JW4212
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12453. rraB.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001697591 – 138Regulator of ribonuclease activity BAdd BLAST138

Proteomic databases

PaxDbiP0AF90.
PRIDEiP0AF90.

Expressioni

Inductioni

Constitutively expressed. Expression is increased by a reduction in intracellular concentration of UDP-GlcNAc.1 Publication

Interactioni

Subunit structurei

Interacts with the C-terminal region of Rne.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
glnBP0A9Z12EBI-544031,EBI-551053

GO - Molecular functioni

  • enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263244. 5 interactors.
DIPiDIP-47905N.
IntActiP0AF90. 16 interactors.
MINTiMINT-1243800.
STRINGi511145.b4255.

Structurei

3D structure databases

ProteinModelPortaliP0AF90.
SMRiP0AF90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi116 – 134Asp/Glu-rich (acidic)Add BLAST19

Sequence similaritiesi

Belongs to the RraB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KQT. Bacteria.
COG3076. LUCA.
HOGENOMiHOG000282487.
KOiK09893.
OMAiFCFDAIA.

Family and domain databases

Gene3Di3.30.70.970. 1 hit.
HAMAPiMF_01888. RraB. 1 hit.
InterProiIPR016716. RraB.
IPR009671. RraB_dom.
[Graphical view]
PfamiPF06877. RraB. 1 hit.
[Graphical view]
PIRSFiPIRSF018193. UCP018193. 1 hit.
SUPFAMiSSF89946. SSF89946. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AF90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPEQLEEQ REETRLIIEE LLEDGSDPDA LYTIEHHLSA DDLETLEKAA
60 70 80 90 100
VEAFKLGYEV TDPEELEVED GDIVICCDIL SECALNADLI DAQVEQLMTL
110 120 130
AEKFDVEYDG WGTYFEDPNG EDGDDEDFVD EDDDGVRH
Length:138
Mass (Da):15,603
Last modified:December 20, 2005 - v1
Checksum:iE23D6DBE6547FD0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97151.1.
U00096 Genomic DNA. Translation: AAC77212.1.
AP009048 Genomic DNA. Translation: BAE78252.1.
M24186 Genomic DNA. No translation available.
PIRiS56480.
RefSeqiNP_418676.1. NC_000913.3.
WP_000002953.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77212; AAC77212; b4255.
BAE78252; BAE78252; BAE78252.
GeneIDi948773.
KEGGiecj:JW4212.
eco:b4255.
PATRICi32124081. VBIEscCol129921_4385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97151.1.
U00096 Genomic DNA. Translation: AAC77212.1.
AP009048 Genomic DNA. Translation: BAE78252.1.
M24186 Genomic DNA. No translation available.
PIRiS56480.
RefSeqiNP_418676.1. NC_000913.3.
WP_000002953.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AF90.
SMRiP0AF90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263244. 5 interactors.
DIPiDIP-47905N.
IntActiP0AF90. 16 interactors.
MINTiMINT-1243800.
STRINGi511145.b4255.

Proteomic databases

PaxDbiP0AF90.
PRIDEiP0AF90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77212; AAC77212; b4255.
BAE78252; BAE78252; BAE78252.
GeneIDi948773.
KEGGiecj:JW4212.
eco:b4255.
PATRICi32124081. VBIEscCol129921_4385.

Organism-specific databases

EchoBASEiEB2347.
EcoGeneiEG12453. rraB.

Phylogenomic databases

eggNOGiENOG4105KQT. Bacteria.
COG3076. LUCA.
HOGENOMiHOG000282487.
KOiK09893.
OMAiFCFDAIA.

Enzyme and pathway databases

BioCyciEcoCyc:G7885-MONOMER.
ECOL316407:JW4212-MONOMER.

Miscellaneous databases

PROiP0AF90.

Family and domain databases

Gene3Di3.30.70.970. 1 hit.
HAMAPiMF_01888. RraB. 1 hit.
InterProiIPR016716. RraB.
IPR009671. RraB_dom.
[Graphical view]
PfamiPF06877. RraB. 1 hit.
[Graphical view]
PIRSFiPIRSF018193. UCP018193. 1 hit.
SUPFAMiSSF89946. SSF89946. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAB_ECOLI
AccessioniPrimary (citable) accession number: P0AF90
Secondary accession number(s): P37163, Q2M654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

RraA and RraB interact with Rne at separate sites within the Rne and exert distinct effects on the composition of the degradosome, affecting distinct sets of RNA transcripts.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.