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Protein

tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

Gene

tsaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.1 Publication

Kineticsi

kcat is 0.0026 sec(-1) for ATP hydrolysis.

  1. KM=169 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei11ATPBy similarity1
    Metal bindingi42MagnesiumBy similarity1
    Metal bindingi108MagnesiumBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi38 – 43ATPBy similarity6

    GO - Molecular functioni

    • ADP binding Source: EcoCyc
    • ATPase activity Source: EcoCyc
    • ATP binding Source: UniProtKB-KW
    • identical protein binding Source: EcoCyc
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • tRNA threonylcarbamoyladenosine modification Source: EcoCyc
    Complete GO annotation...

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11757-MONOMER.
    ECOL316407:JW4126-MONOMER.
    MetaCyc:EG11757-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
    Gene namesi
    Name:tsaE
    Synonyms:yjeE
    Ordered Locus Names:b4168, JW4126
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11757. tsaE.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Appears essential for growth, since no null mutants can be obtained. Conditional depletion of this gene leads to very elongated cells, 1.5 to 1.75 times wider than wild-type, displaying an unusual distribution of the DNA, which appears to be located around the periphery of the cell rather than throughout the cytoplasm. The TsaE depletion phenotype is suppressed by overexpressing the response regulator RstA.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000962051 – 153tRNA threonylcarbamoyladenosine biosynthesis protein TsaEAdd BLAST153

    Proteomic databases

    PaxDbiP0AF67.
    PRIDEiP0AF67.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with TsaB.2 Publications

    GO - Molecular functioni

    • identical protein binding Source: EcoCyc

    Protein-protein interaction databases

    BioGridi4262705. 136 interactors.
    DIPiDIP-47867N.
    IntActiP0AF67. 23 interactors.
    MINTiMINT-1256268.
    STRINGi511145.b4168.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AF67.
    SMRiP0AF67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TsaE family.Curated

    Phylogenomic databases

    eggNOGiENOG4105K8N. Bacteria.
    COG0802. LUCA.
    HOGENOMiHOG000052238.
    InParanoidiP0AF67.
    KOiK06925.
    OMAiLDIDIRY.
    PhylomeDBiP0AF67.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR003442. T6A_TsaE.
    [Graphical view]
    PfamiPF02367. TsaE. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00150. T6A_YjeE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AF67-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMNRVIPLPD EQATLDLGER VAKACDGATV IYLYGDLGAG KTTFSRGFLQ
    60 70 80 90 100
    ALGHQGNVKS PTYTLVEPYT LDNLMVYHFD LYRLADPEEL EFMGIRDYFA
    110 120 130 140 150
    NDAICLVEWP QQGTGVLPDP DVEIHIDYQA QGREARVSAV SSAGELLLAR

    LAG
    Length:153
    Mass (Da):16,853
    Last modified:December 20, 2005 - v1
    Checksum:i49F8C7AA5965EC55
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L19346 Unassigned DNA. Translation: AAA20096.1.
    U14003 Genomic DNA. Translation: AAA97064.1.
    U00096 Genomic DNA. Translation: AAC77125.1.
    AP009048 Genomic DNA. Translation: BAE78169.1.
    PIRiS56393.
    RefSeqiNP_418589.1. NC_000913.3.
    WP_000981977.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77125; AAC77125; b4168.
    BAE78169; BAE78169; BAE78169.
    GeneIDi948684.
    KEGGiecj:JW4126.
    eco:b4168.
    PATRICi32123909. VBIEscCol129921_4299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L19346 Unassigned DNA. Translation: AAA20096.1.
    U14003 Genomic DNA. Translation: AAA97064.1.
    U00096 Genomic DNA. Translation: AAC77125.1.
    AP009048 Genomic DNA. Translation: BAE78169.1.
    PIRiS56393.
    RefSeqiNP_418589.1. NC_000913.3.
    WP_000981977.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0AF67.
    SMRiP0AF67.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262705. 136 interactors.
    DIPiDIP-47867N.
    IntActiP0AF67. 23 interactors.
    MINTiMINT-1256268.
    STRINGi511145.b4168.

    Proteomic databases

    PaxDbiP0AF67.
    PRIDEiP0AF67.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77125; AAC77125; b4168.
    BAE78169; BAE78169; BAE78169.
    GeneIDi948684.
    KEGGiecj:JW4126.
    eco:b4168.
    PATRICi32123909. VBIEscCol129921_4299.

    Organism-specific databases

    EchoBASEiEB1707.
    EcoGeneiEG11757. tsaE.

    Phylogenomic databases

    eggNOGiENOG4105K8N. Bacteria.
    COG0802. LUCA.
    HOGENOMiHOG000052238.
    InParanoidiP0AF67.
    KOiK06925.
    OMAiLDIDIRY.
    PhylomeDBiP0AF67.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11757-MONOMER.
    ECOL316407:JW4126-MONOMER.
    MetaCyc:EG11757-MONOMER.

    Miscellaneous databases

    PROiP0AF67.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR003442. T6A_TsaE.
    [Graphical view]
    PfamiPF02367. TsaE. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00150. T6A_YjeE. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTSAE_ECOLI
    AccessioniPrimary (citable) accession number: P0AF67
    Secondary accession number(s): P31805, Q2M6D7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: November 2, 2016
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    TsaBCDE are necessary and sufficient for tRNA(NNU) t6A37 threonylcarbamoyladenosine modification in vitro in E.coli.1 Publication

    Caution

    The well-known t6A modification appears to be a hydrolyzed artifact of natural cyclic t6A (ct6A) that occurs during the preparation and handling of tRNA in E.coli and many other species (PubMed:23242255). In these species, the t6A modification is processed further by dehydration into ct6A, a reaction catalyzed by TcdA.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.