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Protein

tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

Gene

tsaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.1 Publication

Miscellaneous

TsaBCDE are necessary and sufficient for tRNA(NNU) t6A37 threonylcarbamoyladenosine modification in vitro in E.coli.1 Publication

Caution

The well-known t6A modification appears to be a hydrolyzed artifact of natural cyclic t6A (ct6A) that occurs during the preparation and handling of tRNA in E.coli and many other species (PubMed:23242255). In these species, the t6A modification is processed further by dehydration into ct6A, a reaction catalyzed by TcdA.Curated

Kineticsi

kcat is 0.0026 sec(-1) for ATP hydrolysis.
  1. KM=169 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei11ATPBy similarity1
    Metal bindingi42MagnesiumBy similarity1
    Metal bindingi108MagnesiumBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi38 – 43ATPBy similarity6

    GO - Molecular functioni

    • ADP binding Source: EcoCyc
    • ATPase activity Source: EcoCyc
    • ATP binding Source: UniProtKB-KW
    • identical protein binding Source: EcoCyc
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • tRNA threonylcarbamoyladenosine modification Source: EcoCyc

    Keywordsi

    Biological processtRNA processing
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11757-MONOMER
    MetaCyc:EG11757-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
    Gene namesi
    Name:tsaE
    Synonyms:yjeE
    Ordered Locus Names:b4168, JW4126
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11757 tsaE

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Appears essential for growth, since no null mutants can be obtained. Conditional depletion of this gene leads to very elongated cells, 1.5 to 1.75 times wider than wild-type, displaying an unusual distribution of the DNA, which appears to be located around the periphery of the cell rather than throughout the cytoplasm. The TsaE depletion phenotype is suppressed by overexpressing the response regulator RstA.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000962051 – 153tRNA threonylcarbamoyladenosine biosynthesis protein TsaEAdd BLAST153

    Proteomic databases

    PaxDbiP0AF67
    PRIDEiP0AF67

    Interactioni

    Subunit structurei

    Homodimer. Interacts with TsaB.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rlmBP631773EBI-561268,EBI-562712

    GO - Molecular functioni

    • identical protein binding Source: EcoCyc

    Protein-protein interaction databases

    BioGridi4262705, 155 interactors
    DIPiDIP-47867N
    IntActiP0AF67, 23 interactors
    STRINGi316385.ECDH10B_4363

    Structurei

    3D structure databases

    ProteinModelPortaliP0AF67
    SMRiP0AF67
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TsaE family.Curated

    Phylogenomic databases

    eggNOGiENOG4105K8N Bacteria
    COG0802 LUCA
    HOGENOMiHOG000052238
    InParanoidiP0AF67
    KOiK06925
    OMAiCLIEWAE
    PhylomeDBiP0AF67

    Family and domain databases

    InterProiView protein in InterPro
    IPR027417 P-loop_NTPase
    IPR003442 T6A_TsaE
    PfamiView protein in Pfam
    PF02367 TsaE, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    TIGRFAMsiTIGR00150 T6A_YjeE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0AF67-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMNRVIPLPD EQATLDLGER VAKACDGATV IYLYGDLGAG KTTFSRGFLQ
    60 70 80 90 100
    ALGHQGNVKS PTYTLVEPYT LDNLMVYHFD LYRLADPEEL EFMGIRDYFA
    110 120 130 140 150
    NDAICLVEWP QQGTGVLPDP DVEIHIDYQA QGREARVSAV SSAGELLLAR

    LAG
    Length:153
    Mass (Da):16,853
    Last modified:December 20, 2005 - v1
    Checksum:i49F8C7AA5965EC55
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L19346 Unassigned DNA Translation: AAA20096.1
    U14003 Genomic DNA Translation: AAA97064.1
    U00096 Genomic DNA Translation: AAC77125.1
    AP009048 Genomic DNA Translation: BAE78169.1
    PIRiS56393
    RefSeqiNP_418589.1, NC_000913.3
    WP_000981977.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77125; AAC77125; b4168
    BAE78169; BAE78169; BAE78169
    GeneIDi948684
    KEGGiecj:JW4126
    eco:b4168
    PATRICifig|1411691.4.peg.2533

    Similar proteinsi

    Entry informationi

    Entry nameiTSAE_ECOLI
    AccessioniPrimary (citable) accession number: P0AF67
    Secondary accession number(s): P31805, Q2M6D7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: March 28, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

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