Reviewed, UniProtKB/Swiss-Prot P0AF63 (NSRR_ECOLI)

Names and origin

Protein names

Recommended name:
    HTH-type transcriptional repressor nsrR

Gene names

Name:	nsrR
Synonyms:	yjeB
Ordered Locus Names:	b4178, JW4136

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Nitric oxide-sensitive repressor of genes involved in protecting the cell against nitrosative stress, such as ytfE, hmpA and ygbA. May require iron for activity. Does not regulates its own transcription. Ref.5
Cofactor	Binds 1 2Fe-2S cluster per subunit Potential.
Sequence similarities	Contains 1 HTH rrf2-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	2Fe-2S DNA-binding Iron Iron-sulfur Metal-binding
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded DNA binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
prs	P0A717	1	EBI-1134945,EBI-906827

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	HTH-type transcriptional repressor nsrR HAMAP MF_01177		PRO_0000109555
Regions
	Domain	2 – 129	128	HTH rrf2-type
	DNA binding	28 – 51	24	H-T-H motif By similarity
Sites
	Metal binding	91	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	96	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	102	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AF63-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 6A783840023134D7	FASTA	141	15,593
	10 20 30 40 50 60 MQLTSFTDYG LRALIYMASL PEGRMTSISE VTDVYGVSRN HMVKIINQLS RAGYVTAVRG 70 80 90 100 110 120 KNGGIRLGKP ASAIRIGDVV RELEPLSLVN CSSEFCHITP ACRLKQALSK AVQSFLTELD 130 140 NYTLADLVEE NQPLYKLLLV E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12." Wolfe S.A., Smith J.M. J. Biol. Chem. 263:19147-19153(1988) [PubMed: 3058695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator." Bodenmiller D.M., Spiro S. J. Bacteriol. 188:874-881(2006) [PubMed: 16428390] [Abstract] Cited for: FUNCTION. Strain: K12 / MC1000 / ATCC 39531.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04199 Genomic DNA. No translation available. U14003 Genomic DNA. Translation: AAA97074.1. U00096 Genomic DNA. Translation: AAC77135.1. AP009048 Genomic DNA. Translation: BAE78179.1.
PIR	S56403.
RefSeq	AP_004678.1. NP_418599.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AF63. 1 interaction.
Genome annotation databases
GeneID	948693.
GenomeReviews	Gene locus JW4136 in contig AP009048_GR. Gene locus b4178 in contig U00096_GR.
KEGG	ecj:JW4136. eco:b4178.
Organism-specific databases
EchoBASE	EB1197.
EcoGene	EG11212. nsrR.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AF63.
OMA	P0AF63. DLALVEC.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11212-MON.
Family and domain databases
HAMAP	MF_01177. [Tree]
InterPro	IPR000944. Transcriptional_reg_Rrf2. [Graphical view]
Pfam	PF02082. Rrf2. 1 hit. [Graphical view]
ProDom	PD003632. UPF0074. 1 hit. PD006327. Wing_hlx_tran_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00738. rrf2_super. 1 hit.
PROSITE	PS01332. HTH_RRF2_1. 1 hit. PS51197. HTH_RRF2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSRR_ECOLI

Accession

Primary (citable) accession number: P0AF63
Secondary accession number(s): P21498, P72442, Q2M6C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)