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Protein

Guanine/hypoxanthine permease GhxP

Gene

ghxP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for guanine and hypoxanthine.1 Publication

Kineticsi

  1. KM=1.6 µM for guanine1 Publication
  2. KM=11.2 µM for hypoxanthine1 Publication
  1. Vmax=4.0 nmol/min/mg enzyme with guanine as substrate1 Publication
  2. Vmax=2.7 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication

GO - Molecular functioni

  • guanine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • guanine import into cell Source: EcoCyc
  • hypoxanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11939-MONOMER.
ECOL316407:JW4025-MONOMER.
MetaCyc:EG11939-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.5. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine/hypoxanthine permease GhxP
Gene namesi
Name:ghxP
Synonyms:yjcD
Ordered Locus Names:b4064, JW4025
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11939. ghxP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4924HelicalSequence analysisAdd
BLAST
Topological domaini50 – 5910PeriplasmicSequence analysis
Transmembranei60 – 7819HelicalSequence analysisAdd
BLAST
Topological domaini79 – 802CytoplasmicSequence analysis
Transmembranei81 – 9717Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini98 – 10912PeriplasmicSequence analysisAdd
BLAST
Transmembranei110 – 12920HelicalSequence analysisAdd
BLAST
Topological domaini130 – 14112CytoplasmicSequence analysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Topological domaini163 – 18018PeriplasmicSequence analysisAdd
BLAST
Transmembranei181 – 19818HelicalSequence analysisAdd
BLAST
Topological domaini199 – 2024CytoplasmicSequence analysis
Transmembranei203 – 22220HelicalSequence analysisAdd
BLAST
Topological domaini223 – 25432PeriplasmicSequence analysisAdd
BLAST
Transmembranei255 – 28329HelicalSequence analysisAdd
BLAST
Topological domaini284 – 29613CytoplasmicSequence analysisAdd
BLAST
Transmembranei297 – 31216HelicalSequence analysisAdd
BLAST
Topological domaini313 – 3142PeriplasmicSequence analysis
Transmembranei315 – 33016Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini331 – 3344CytoplasmicSequence analysis
Transmembranei335 – 34915HelicalSequence analysisAdd
BLAST
Topological domaini350 – 36011PeriplasmicSequence analysisAdd
BLAST
Transmembranei361 – 38020HelicalSequence analysisAdd
BLAST
Topological domaini381 – 3855CytoplasmicSequence analysis
Intramembranei386 – 42136Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini422 – 44928CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351T → A: Almost no change in activity. 1 Publication
Mutagenesisi35 – 351T → H: Lack of activity. 1 Publication
Mutagenesisi88 – 881A → G or S: Almost no change in activity. 1 Publication
Mutagenesisi271 – 2711D → A or N: Strong decrease in activity. 1 Publication
Mutagenesisi271 – 2711D → E: Lack of activity. 1 Publication
Mutagenesisi275 – 2751T → A, D or N: Lack of activity. 1 Publication
Mutagenesisi275 – 2751T → S: Strong decrease in activity. 1 Publication
Mutagenesisi302 – 3021D → E: Lack of activity. 1 Publication
Mutagenesisi302 – 3021D → N: Almost no change in activity. 1 Publication
Mutagenesisi321 – 3211I → A: Lack of activity. 1 Publication
Mutagenesisi321 – 3211I → E: Almost no change in activity. 1 Publication
Mutagenesisi322 – 3221E → D: Strong decrease in activity. 1 Publication
Mutagenesisi322 – 3221E → Q: Lack of activity. 1 Publication
Mutagenesisi323 – 3231S → A or N: Lack of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Guanine/hypoxanthine permease GhxPPRO_0000169720Add
BLAST

Proteomic databases

PaxDbiP0AF52.

Interactioni

Protein-protein interaction databases

BioGridi4262672. 290 interactions.
DIPiDIP-48113N.
IntActiP0AF52. 1 interaction.
MINTiMINT-1260311.
STRINGi511145.b4064.

Structurei

3D structure databases

ProteinModelPortaliP0AF52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiP0AF52.
KOiK06901.
OMAiLMGVWAR.
OrthoDBiEOG6DNT97.
PhylomeDBiP0AF52.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSARTGG SLDAWFKISQ RGSTVRQEVV AGLTTFLAMV YSVIVVPGML
60 70 80 90 100
GKAGFPPAAV FVATCLVAGL GSIVMGLWAN LPLAIGCAIS LTAFTAFSLV
110 120 130 140 150
LGQHISVPVA LGAVFLMGVL FTVISATGIR SWILRNLPHG VAHGTGIGIG
160 170 180 190 200
LFLLLIAANG VGLVIKNPLD GLPVALGDFA TFPVIMSLVG LAVIIGLEKL
210 220 230 240 250
KVPGGILLTI IGISIVGLIF DPNVHFSGVF AMPSLSDENG NSLIGSLDIM
260 270 280 290 300
GALNPVVLPS VLALVMTAVF DATGTIRAVA GQANLLDKDG QIIDGGKALT
310 320 330 340 350
TDSMSSVFSG LVGAAPAAVY IESAAGTAAG GKTGLTAITV GVLFLLILFL
360 370 380 390 400
SPLSYLVPGY ATAPALMYVG LLMLSNVAKI DFADFVDAMA GLVTAVFIVL
410 420 430 440
TCNIVTGIMI GFATLVIGRL VSGEWRKLNI GTVVIAVALV TFYAGGWAI
Length:449
Mass (Da):45,711
Last modified:December 20, 2005 - v1
Checksum:iBDBFE2F057E45910
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43158.1.
U00096 Genomic DNA. Translation: AAC77034.1.
AP009048 Genomic DNA. Translation: BAE78066.1.
PIRiG65214.
RefSeqiNP_418488.1. NC_000913.3.
WP_000106882.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77034; AAC77034; b4064.
BAE78066; BAE78066; BAE78066.
GeneIDi948565.
KEGGiecj:JW4025.
eco:b4064.
PATRICi32123675. VBIEscCol129921_4186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43158.1.
U00096 Genomic DNA. Translation: AAC77034.1.
AP009048 Genomic DNA. Translation: BAE78066.1.
PIRiG65214.
RefSeqiNP_418488.1. NC_000913.3.
WP_000106882.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AF52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262672. 290 interactions.
DIPiDIP-48113N.
IntActiP0AF52. 1 interaction.
MINTiMINT-1260311.
STRINGi511145.b4064.

Protein family/group databases

TCDBi2.A.40.7.5. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP0AF52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77034; AAC77034; b4064.
BAE78066; BAE78066; BAE78066.
GeneIDi948565.
KEGGiecj:JW4025.
eco:b4064.
PATRICi32123675. VBIEscCol129921_4186.

Organism-specific databases

EchoBASEiEB1883.
EcoGeneiEG11939. ghxP.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiP0AF52.
KOiK06901.
OMAiLMGVWAR.
OrthoDBiEOG6DNT97.
PhylomeDBiP0AF52.

Enzyme and pathway databases

BioCyciEcoCyc:EG11939-MONOMER.
ECOL316407:JW4025-MONOMER.
MetaCyc:EG11939-MONOMER.

Miscellaneous databases

PROiP0AF52.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Functional identification of the hypoxanthine/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12."
    Papakostas K., Botou M., Frillingos S.
    J. Biol. Chem. 288:36827-36840(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TOPOLOGY, GENE NAME, MUTAGENESIS OF THR-35; ALA-88; ASP-271; THR-275; ASP-302; ILE-321; GLU-322 AND SER-323.
    Strain: K12.

Entry informationi

Entry nameiGHXP_ECOLI
AccessioniPrimary (citable) accession number: P0AF52
Secondary accession number(s): P32702, Q2M6P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.