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Protein

Guanine/hypoxanthine permease GhxP

Gene

ghxP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for guanine and hypoxanthine.1 Publication

Kineticsi

  1. KM=1.6 µM for guanine1 Publication
  2. KM=11.2 µM for hypoxanthine1 Publication
  1. Vmax=4.0 nmol/min/mg enzyme with guanine as substrate1 Publication
  2. Vmax=2.7 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication

GO - Molecular functioni

  • guanine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • guanine import into cell Source: EcoCyc
  • hypoxanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11939-MONOMER.
ECOL316407:JW4025-MONOMER.
MetaCyc:EG11939-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.5. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine/hypoxanthine permease GhxP
Gene namesi
Name:ghxP
Synonyms:yjcD
Ordered Locus Names:b4064, JW4025
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11939. ghxP.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 49HelicalSequence analysisAdd BLAST24
Topological domaini50 – 59PeriplasmicSequence analysis10
Transmembranei60 – 78HelicalSequence analysisAdd BLAST19
Topological domaini79 – 80CytoplasmicSequence analysis2
Transmembranei81 – 97Discontinuously helicalSequence analysisAdd BLAST17
Topological domaini98 – 109PeriplasmicSequence analysisAdd BLAST12
Transmembranei110 – 129HelicalSequence analysisAdd BLAST20
Topological domaini130 – 141CytoplasmicSequence analysisAdd BLAST12
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Topological domaini163 – 180PeriplasmicSequence analysisAdd BLAST18
Transmembranei181 – 198HelicalSequence analysisAdd BLAST18
Topological domaini199 – 202CytoplasmicSequence analysis4
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 254PeriplasmicSequence analysisAdd BLAST32
Transmembranei255 – 283HelicalSequence analysisAdd BLAST29
Topological domaini284 – 296CytoplasmicSequence analysisAdd BLAST13
Transmembranei297 – 312HelicalSequence analysisAdd BLAST16
Topological domaini313 – 314PeriplasmicSequence analysis2
Transmembranei315 – 330Discontinuously helicalSequence analysisAdd BLAST16
Topological domaini331 – 334CytoplasmicSequence analysis4
Transmembranei335 – 349HelicalSequence analysisAdd BLAST15
Topological domaini350 – 360PeriplasmicSequence analysisAdd BLAST11
Transmembranei361 – 380HelicalSequence analysisAdd BLAST20
Topological domaini381 – 385CytoplasmicSequence analysis5
Intramembranei386 – 421Discontinuously helicalSequence analysisAdd BLAST36
Topological domaini422 – 449CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35T → A: Almost no change in activity. 1 Publication1
Mutagenesisi35T → H: Lack of activity. 1 Publication1
Mutagenesisi88A → G or S: Almost no change in activity. 1 Publication1
Mutagenesisi271D → A or N: Strong decrease in activity. 1 Publication1
Mutagenesisi271D → E: Lack of activity. 1 Publication1
Mutagenesisi275T → A, D or N: Lack of activity. 1 Publication1
Mutagenesisi275T → S: Strong decrease in activity. 1 Publication1
Mutagenesisi302D → E: Lack of activity. 1 Publication1
Mutagenesisi302D → N: Almost no change in activity. 1 Publication1
Mutagenesisi321I → A: Lack of activity. 1 Publication1
Mutagenesisi321I → E: Almost no change in activity. 1 Publication1
Mutagenesisi322E → D: Strong decrease in activity. 1 Publication1
Mutagenesisi322E → Q: Lack of activity. 1 Publication1
Mutagenesisi323S → A or N: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001697201 – 449Guanine/hypoxanthine permease GhxPAdd BLAST449

Proteomic databases

PaxDbiP0AF52.
PRIDEiP0AF52.

Interactioni

Protein-protein interaction databases

BioGridi4262672. 290 interactors.
DIPiDIP-48113N.
IntActiP0AF52. 1 interactor.
MINTiMINT-1260311.
STRINGi511145.b4064.

Structurei

3D structure databases

ProteinModelPortaliP0AF52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiP0AF52.
KOiK06901.
OMAiLFKFEER.
PhylomeDBiP0AF52.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSARTGG SLDAWFKISQ RGSTVRQEVV AGLTTFLAMV YSVIVVPGML
60 70 80 90 100
GKAGFPPAAV FVATCLVAGL GSIVMGLWAN LPLAIGCAIS LTAFTAFSLV
110 120 130 140 150
LGQHISVPVA LGAVFLMGVL FTVISATGIR SWILRNLPHG VAHGTGIGIG
160 170 180 190 200
LFLLLIAANG VGLVIKNPLD GLPVALGDFA TFPVIMSLVG LAVIIGLEKL
210 220 230 240 250
KVPGGILLTI IGISIVGLIF DPNVHFSGVF AMPSLSDENG NSLIGSLDIM
260 270 280 290 300
GALNPVVLPS VLALVMTAVF DATGTIRAVA GQANLLDKDG QIIDGGKALT
310 320 330 340 350
TDSMSSVFSG LVGAAPAAVY IESAAGTAAG GKTGLTAITV GVLFLLILFL
360 370 380 390 400
SPLSYLVPGY ATAPALMYVG LLMLSNVAKI DFADFVDAMA GLVTAVFIVL
410 420 430 440
TCNIVTGIMI GFATLVIGRL VSGEWRKLNI GTVVIAVALV TFYAGGWAI
Length:449
Mass (Da):45,711
Last modified:December 20, 2005 - v1
Checksum:iBDBFE2F057E45910
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43158.1.
U00096 Genomic DNA. Translation: AAC77034.1.
AP009048 Genomic DNA. Translation: BAE78066.1.
PIRiG65214.
RefSeqiNP_418488.1. NC_000913.3.
WP_000106882.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77034; AAC77034; b4064.
BAE78066; BAE78066; BAE78066.
GeneIDi948565.
KEGGiecj:JW4025.
eco:b4064.
PATRICi32123675. VBIEscCol129921_4186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43158.1.
U00096 Genomic DNA. Translation: AAC77034.1.
AP009048 Genomic DNA. Translation: BAE78066.1.
PIRiG65214.
RefSeqiNP_418488.1. NC_000913.3.
WP_000106882.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AF52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262672. 290 interactors.
DIPiDIP-48113N.
IntActiP0AF52. 1 interactor.
MINTiMINT-1260311.
STRINGi511145.b4064.

Protein family/group databases

TCDBi2.A.40.7.5. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP0AF52.
PRIDEiP0AF52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77034; AAC77034; b4064.
BAE78066; BAE78066; BAE78066.
GeneIDi948565.
KEGGiecj:JW4025.
eco:b4064.
PATRICi32123675. VBIEscCol129921_4186.

Organism-specific databases

EchoBASEiEB1883.
EcoGeneiEG11939. ghxP.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiP0AF52.
KOiK06901.
OMAiLFKFEER.
PhylomeDBiP0AF52.

Enzyme and pathway databases

BioCyciEcoCyc:EG11939-MONOMER.
ECOL316407:JW4025-MONOMER.
MetaCyc:EG11939-MONOMER.

Miscellaneous databases

PROiP0AF52.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGHXP_ECOLI
AccessioniPrimary (citable) accession number: P0AF52
Secondary accession number(s): P32702, Q2M6P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.