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Protein

Cell division protein ZapB

Gene

zapB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-essential, abundant cell division factor that is required for proper Z-ring formation. It is recruited early to the divisome by direct interaction with FtsZ, stimulating Z-ring assembly and thereby promoting cell division earlier in the cell cycle. Its recruitment to the Z-ring requires functional FtsA or ZipA.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

GO - Biological processi

  • barrier septum assembly Source: UniProtKB-HAMAP
  • FtsZ-dependent cytokinesis Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Septation

Enzyme and pathway databases

BioCyciEcoCyc:EG11878-MONOMER.
ECOL316407:JW3899-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein ZapB
Gene namesi
Name:zapB
Synonyms:yiiU
Ordered Locus Names:b3928, JW3899
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11878. yiiU.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Localizes to the septum at mid-cell, in a FtsZ-like pattern.

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Cell division protein ZapBPRO_0000169693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0AF36.
PaxDbiP0AF36.
PRIDEiP0AF36.

Interactioni

Subunit structurei

Homodimer. The ends of the coiled-coil dimer bind to each other, forming polymers. Interacts with FtsZ.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
zapAP0ADS24EBI-1134093,EBI-1119901

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262648. 241 interactions.
DIPiDIP-35850N.
IntActiP0AF36. 4 interactions.
STRINGi511145.b3928.

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 7874Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JEEX-ray2.80A/B/C/D1-81[»]
ProteinModelPortaliP0AF36.
SMRiP0AF36. Positions 3-80.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AF36.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili5 – 8177Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the ZapB family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105N4M. Bacteria.
COG3074. LUCA.
HOGENOMiHOG000218436.
KOiK09892.
OMAiGKMEDVQ.
OrthoDBiEOG6P8TT4.

Family and domain databases

HAMAPiMF_01196. ZapB.
InterProiIPR009252. Cell_div_ZapB.
[Graphical view]
PfamiPF06005. DUF904. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMSLEVFEK LEAKVQQAID TITLLQMEIE ELKEKNNSLS QEVQNAQHQR
60 70 80
EELERENNHL KEQQNGWQER LQALLGRMEE V
Length:81
Mass (Da):9,635
Last modified:December 20, 2005 - v1
Checksum:iB01DC1A433A1D01B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03060.1.
U00096 Genomic DNA. Translation: AAC76910.1.
AP009048 Genomic DNA. Translation: BAE77382.1.
PIRiS40871.
RefSeqiNP_418363.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76910; AAC76910; b3928.
BAE77382; BAE77382; BAE77382.
GeneIDi948420.
KEGGiecj:JW3899.
eco:b3928.
PATRICi32123371. VBIEscCol129921_4046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03060.1.
U00096 Genomic DNA. Translation: AAC76910.1.
AP009048 Genomic DNA. Translation: BAE77382.1.
PIRiS40871.
RefSeqiNP_418363.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JEEX-ray2.80A/B/C/D1-81[»]
ProteinModelPortaliP0AF36.
SMRiP0AF36. Positions 3-80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262648. 241 interactions.
DIPiDIP-35850N.
IntActiP0AF36. 4 interactions.
STRINGi511145.b3928.

Proteomic databases

EPDiP0AF36.
PaxDbiP0AF36.
PRIDEiP0AF36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76910; AAC76910; b3928.
BAE77382; BAE77382; BAE77382.
GeneIDi948420.
KEGGiecj:JW3899.
eco:b3928.
PATRICi32123371. VBIEscCol129921_4046.

Organism-specific databases

EchoBASEiEB1824.
EcoGeneiEG11878. yiiU.

Phylogenomic databases

eggNOGiENOG4105N4M. Bacteria.
COG3074. LUCA.
HOGENOMiHOG000218436.
KOiK09892.
OMAiGKMEDVQ.
OrthoDBiEOG6P8TT4.

Enzyme and pathway databases

BioCyciEcoCyc:EG11878-MONOMER.
ECOL316407:JW3899-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AF36.
PROiP0AF36.

Family and domain databases

HAMAPiMF_01196. ZapB.
InterProiIPR009252. Cell_div_ZapB.
[Graphical view]
PfamiPF06005. DUF904. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
    Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
    Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-10, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
  5. "Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division."
    Ebersbach G., Galli E., Moeller-Jensen J., Loewe J., Gerdes K.
    Mol. Microbiol. 68:720-735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CELL DIVISION, COILED-COIL DOMAIN, SUBUNIT, INTERACTION WITH FTSZ, SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiZAPB_ECOLI
AccessioniPrimary (citable) accession number: P0AF36
Secondary accession number(s): P32164, Q2M8M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 16, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.