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P0AF33

- NARV_ECO57

UniProt

P0AF33 - NARV_ECO57

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Protein
Respiratory nitrate reductase 2 gamma chain
Gene
narV, Z2247, ECs2068
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit By similarity.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron (heme B 1 axial ligand) By similarity
Metal bindingi67 – 671Iron (heme B 2 axial ligand) By similarity
Metal bindingi188 – 1881Iron (heme B 2 axial ligand) By similarity
Metal bindingi206 – 2061Iron (heme B 1 axial ligand) By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2040-MONOMER.
ECOO157:NARV-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 2 gamma chain (EC:1.7.99.4)
Gene namesi
Name:narV
Ordered Locus Names:Z2247, ECs2068
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 43Periplasmic By similarity
Transmembranei5 – 3026Helical; Name=1; By similarity
Add
BLAST
Topological domaini31 – 4818Cytoplasmic By similarity
Add
BLAST
Transmembranei49 – 7123Helical; Name=2; By similarity
Add
BLAST
Topological domaini72 – 8312Periplasmic By similarity
Add
BLAST
Transmembranei84 – 11330Helical; Name=3; By similarity
Add
BLAST
Topological domaini114 – 12512Cytoplasmic By similarity
Add
BLAST
Transmembranei126 – 14924Helical; Name=4; By similarity
Add
BLAST
Topological domaini150 – 18334Periplasmic By similarity
Add
BLAST
Transmembranei184 – 19916Helical; Name=5; By similarity
Add
BLAST
Topological domaini200 – 22627Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. nitrate reductase complex Source: InterPro
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Respiratory nitrate reductase 2 gamma chain
PRO_0000096732Add
BLAST

Interactioni

Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane By similarity.

Protein-protein interaction databases

STRINGi155864.Z2247.

Structurei

3D structure databases

ProteinModelPortaliP0AF33.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181.
HOGENOMiHOG000237376.
KOiK00374.
OMAiWTEAVGV.
OrthoDBiEOG6K13W6.

Family and domain databases

InterProiIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamiPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMiSSF103501. SSF103501. 1 hit.
TIGRFAMsiTIGR00351. narI. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AF33-1 [UniParc]FASTAAdd to Basket

« Hide

MIQYLNVFFY DIYPYICATV FFLGSWLRYD YGQYTWRASS SQMLDKRGMV    50
IWSNLFHIGI LGIFFGHLFG MLTPHWMYAW FLPVAAKQLM AMVLGGICGV 100
LTLIGGAGLL WRRLTNQRVR ATSTTPDIII MSILLIQCLL GLSTIPFSAQ 150
YPDGSEMMKL VGWAQSIVTF RGGSSEMLNG VAFVFRLHLV LGMTIFLLFP 200
FTRLVHVWSA PFEYFTRRYQ IVRSRR 226
Length:226
Mass (Da):26,018
Last modified:December 20, 2005 - v1
Checksum:i1D5FAC307F3D5B84
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56305.1.
BA000007 Genomic DNA. Translation: BAB35491.1.
PIRiD90887.
E85730.
RefSeqiNP_287691.1. NC_002655.2.
NP_310095.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56305; AAG56305; Z2247.
BAB35491; BAB35491; BAB35491.
GeneIDi917268.
960731.
KEGGiece:Z2247.
ecs:ECs2068.
PATRICi18353209. VBIEscCol44059_1824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56305.1 .
BA000007 Genomic DNA. Translation: BAB35491.1 .
PIRi D90887.
E85730.
RefSeqi NP_287691.1. NC_002655.2.
NP_310095.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AF33.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z2247.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56305 ; AAG56305 ; Z2247 .
BAB35491 ; BAB35491 ; BAB35491 .
GeneIDi 917268.
960731.
KEGGi ece:Z2247.
ecs:ECs2068.
PATRICi 18353209. VBIEscCol44059_1824.

Phylogenomic databases

eggNOGi COG2181.
HOGENOMi HOG000237376.
KOi K00374.
OMAi WTEAVGV.
OrthoDBi EOG6K13W6.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-2040-MONOMER.
ECOO157:NARV-MONOMER.

Family and domain databases

InterProi IPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view ]
Pfami PF02665. Nitrate_red_gam. 1 hit.
[Graphical view ]
SUPFAMi SSF103501. SSF103501. 1 hit.
TIGRFAMsi TIGR00351. narI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiNARV_ECO57
AccessioniPrimary (citable) accession number: P0AF33
Secondary accession number(s): P19316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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