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P0AF32

- NARV_ECOLI

UniProt

P0AF32 - NARV_ECOLI

Protein

Respiratory nitrate reductase 2 gamma chain

Gene

narV

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron (heme B 1 axial ligand)By similarity
    Metal bindingi67 – 671Iron (heme B 2 axial ligand)By similarity
    Metal bindingi188 – 1881Iron (heme B 2 axial ligand)By similarity
    Metal bindingi206 – 2061Iron (heme B 1 axial ligand)By similarity

    GO - Molecular functioni

    1. electron carrier activity Source: EcoCyc
    2. metal ion binding Source: UniProtKB-KW
    3. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. anaerobic respiration Source: EcoCyc
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:NARV-MONOMER.
    ECOL316407:JW1460-MONOMER.
    MetaCyc:NARV-MONOMER.

    Protein family/group databases

    TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory nitrate reductase 2 gamma chain (EC:1.7.99.4)
    Gene namesi
    Name:narV
    Ordered Locus Names:b1465, JW1460
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10644. narV.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. nitrate reductase complex Source: InterPro
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 226226Respiratory nitrate reductase 2 gamma chainPRO_0000096731Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AF32.

    Interactioni

    Subunit structurei

    Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

    Protein-protein interaction databases

    STRINGi511145.b1465.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AF32.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 43PeriplasmicBy similarity
    Topological domaini31 – 4818CytoplasmicBy similarityAdd
    BLAST
    Topological domaini72 – 8312PeriplasmicBy similarityAdd
    BLAST
    Topological domaini114 – 12512CytoplasmicBy similarityAdd
    BLAST
    Topological domaini150 – 18334PeriplasmicBy similarityAdd
    BLAST
    Topological domaini200 – 22627CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 3026Helical; Name=1By similarityAdd
    BLAST
    Transmembranei49 – 7123Helical; Name=2By similarityAdd
    BLAST
    Transmembranei84 – 11330Helical; Name=3By similarityAdd
    BLAST
    Transmembranei126 – 14924Helical; Name=4By similarityAdd
    BLAST
    Transmembranei184 – 19916Helical; Name=5By similarityAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2181.
    HOGENOMiHOG000237376.
    KOiK00374.
    OMAiWTEAVGV.
    OrthoDBiEOG6K13W6.
    PhylomeDBiP0AF32.

    Family and domain databases

    InterProiIPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view]
    PfamiPF02665. Nitrate_red_gam. 1 hit.
    [Graphical view]
    SUPFAMiSSF103501. SSF103501. 1 hit.
    TIGRFAMsiTIGR00351. narI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AF32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQYLNVFFY DIYPYICATV FFLGSWLRYD YGQYTWRASS SQMLDKRGMV    50
    IWSNLFHIGI LGIFFGHLFG MLTPHWMYAW FLPVAAKQLM AMVLGGICGV 100
    LTLIGGAGLL WRRLTNQRVR ATSTTPDIII MSILLIQCLL GLSTIPFSAQ 150
    YPDGSEMMKL VGWAQSIVTF RGGSSEMLNG VAFVFRLHLV LGMTIFLLFP 200
    FTRLVHVWSA PFEYFTRRYQ IVRSRR 226
    Length:226
    Mass (Da):26,018
    Last modified:December 20, 2005 - v1
    Checksum:i1D5FAC307F3D5B84
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17110 Genomic DNA. Translation: CAA34967.1.
    U00096 Genomic DNA. Translation: AAC74547.1.
    AP009048 Genomic DNA. Translation: BAA15102.1.
    PIRiS11430.
    RefSeqiNP_415982.1. NC_000913.3.
    YP_489730.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74547; AAC74547; b1465.
    BAA15102; BAA15102; BAA15102.
    GeneIDi12934343.
    946029.
    KEGGiecj:Y75_p1441.
    eco:b1465.
    PATRICi32118222. VBIEscCol129921_1531.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17110 Genomic DNA. Translation: CAA34967.1 .
    U00096 Genomic DNA. Translation: AAC74547.1 .
    AP009048 Genomic DNA. Translation: BAA15102.1 .
    PIRi S11430.
    RefSeqi NP_415982.1. NC_000913.3.
    YP_489730.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AF32.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b1465.

    Protein family/group databases

    TCDBi 5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74547 ; AAC74547 ; b1465 .
    BAA15102 ; BAA15102 ; BAA15102 .
    GeneIDi 12934343.
    946029.
    KEGGi ecj:Y75_p1441.
    eco:b1465.
    PATRICi 32118222. VBIEscCol129921_1531.

    Organism-specific databases

    EchoBASEi EB0638.
    EcoGenei EG10644. narV.

    Phylogenomic databases

    eggNOGi COG2181.
    HOGENOMi HOG000237376.
    KOi K00374.
    OMAi WTEAVGV.
    OrthoDBi EOG6K13W6.
    PhylomeDBi P0AF32.

    Enzyme and pathway databases

    BioCyci EcoCyc:NARV-MONOMER.
    ECOL316407:JW1460-MONOMER.
    MetaCyc:NARV-MONOMER.

    Miscellaneous databases

    PROi P0AF32.

    Gene expression databases

    Genevestigatori P0AF32.

    Family and domain databases

    InterProi IPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view ]
    Pfami PF02665. Nitrate_red_gam. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103501. SSF103501. 1 hit.
    TIGRFAMsi TIGR00351. narI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
      Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
      Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiNARV_ECOLI
    AccessioniPrimary (citable) accession number: P0AF32
    Secondary accession number(s): P19316
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene

    External Data

    Dasty 3