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P0AF32

- NARV_ECOLI

UniProt

P0AF32 - NARV_ECOLI

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Protein

Respiratory nitrate reductase 2 gamma chain

Gene

narV

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron (heme B 1 axial ligand)By similarity
Metal bindingi67 – 671Iron (heme B 2 axial ligand)By similarity
Metal bindingi188 – 1881Iron (heme B 2 axial ligand)By similarity
Metal bindingi206 – 2061Iron (heme B 1 axial ligand)By similarity

GO - Molecular functioni

  1. electron carrier activity Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
  3. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. anaerobic respiration Source: EcoCyc
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARV-MONOMER.
ECOL316407:JW1460-MONOMER.
MetaCyc:NARV-MONOMER.

Protein family/group databases

TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 2 gamma chain (EC:1.7.99.4)
Gene namesi
Name:narV
Ordered Locus Names:b1465, JW1460
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10644. narV.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. nitrate reductase complex Source: InterPro
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Respiratory nitrate reductase 2 gamma chainPRO_0000096731Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AF32.

Interactioni

Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

Protein-protein interaction databases

STRINGi511145.b1465.

Structurei

3D structure databases

ProteinModelPortaliP0AF32.
SMRiP0AF32. Positions 3-225.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 43PeriplasmicBy similarity
Topological domaini31 – 4818CytoplasmicBy similarityAdd
BLAST
Topological domaini72 – 8312PeriplasmicBy similarityAdd
BLAST
Topological domaini114 – 12512CytoplasmicBy similarityAdd
BLAST
Topological domaini150 – 18334PeriplasmicBy similarityAdd
BLAST
Topological domaini200 – 22627CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 3026Helical; Name=1By similarityAdd
BLAST
Transmembranei49 – 7123Helical; Name=2By similarityAdd
BLAST
Transmembranei84 – 11330Helical; Name=3By similarityAdd
BLAST
Transmembranei126 – 14924Helical; Name=4By similarityAdd
BLAST
Transmembranei184 – 19916Helical; Name=5By similarityAdd
BLAST

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181.
HOGENOMiHOG000237376.
InParanoidiP0AF32.
KOiK00374.
OMAiWTEAVGV.
OrthoDBiEOG6K13W6.
PhylomeDBiP0AF32.

Family and domain databases

InterProiIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamiPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMiSSF103501. SSF103501. 1 hit.
TIGRFAMsiTIGR00351. narI. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AF32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIQYLNVFFY DIYPYICATV FFLGSWLRYD YGQYTWRASS SQMLDKRGMV
60 70 80 90 100
IWSNLFHIGI LGIFFGHLFG MLTPHWMYAW FLPVAAKQLM AMVLGGICGV
110 120 130 140 150
LTLIGGAGLL WRRLTNQRVR ATSTTPDIII MSILLIQCLL GLSTIPFSAQ
160 170 180 190 200
YPDGSEMMKL VGWAQSIVTF RGGSSEMLNG VAFVFRLHLV LGMTIFLLFP
210 220
FTRLVHVWSA PFEYFTRRYQ IVRSRR
Length:226
Mass (Da):26,018
Last modified:December 20, 2005 - v1
Checksum:i1D5FAC307F3D5B84
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17110 Genomic DNA. Translation: CAA34967.1.
U00096 Genomic DNA. Translation: AAC74547.1.
AP009048 Genomic DNA. Translation: BAA15102.1.
PIRiS11430.
RefSeqiNP_415982.1. NC_000913.3.
YP_489730.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74547; AAC74547; b1465.
BAA15102; BAA15102; BAA15102.
GeneIDi12934343.
946029.
KEGGiecj:Y75_p1441.
eco:b1465.
PATRICi32118222. VBIEscCol129921_1531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17110 Genomic DNA. Translation: CAA34967.1 .
U00096 Genomic DNA. Translation: AAC74547.1 .
AP009048 Genomic DNA. Translation: BAA15102.1 .
PIRi S11430.
RefSeqi NP_415982.1. NC_000913.3.
YP_489730.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AF32.
SMRi P0AF32. Positions 3-225.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1465.

Protein family/group databases

TCDBi 5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74547 ; AAC74547 ; b1465 .
BAA15102 ; BAA15102 ; BAA15102 .
GeneIDi 12934343.
946029.
KEGGi ecj:Y75_p1441.
eco:b1465.
PATRICi 32118222. VBIEscCol129921_1531.

Organism-specific databases

EchoBASEi EB0638.
EcoGenei EG10644. narV.

Phylogenomic databases

eggNOGi COG2181.
HOGENOMi HOG000237376.
InParanoidi P0AF32.
KOi K00374.
OMAi WTEAVGV.
OrthoDBi EOG6K13W6.
PhylomeDBi P0AF32.

Enzyme and pathway databases

BioCyci EcoCyc:NARV-MONOMER.
ECOL316407:JW1460-MONOMER.
MetaCyc:NARV-MONOMER.

Miscellaneous databases

PROi P0AF32.

Gene expression databases

Genevestigatori P0AF32.

Family and domain databases

InterProi IPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view ]
Pfami PF02665. Nitrate_red_gam. 1 hit.
[Graphical view ]
SUPFAMi SSF103501. SSF103501. 1 hit.
TIGRFAMsi TIGR00351. narI. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
    Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
    Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNARV_ECOLI
AccessioniPrimary (citable) accession number: P0AF32
Secondary accession number(s): P19316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3