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P0AF32

- NARV_ECOLI

UniProt

P0AF32 - NARV_ECOLI

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Protein
Respiratory nitrate reductase 2 gamma chain
Gene
narV, b1465, JW1460
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron (heme B 1 axial ligand) By similarity
Metal bindingi67 – 671Iron (heme B 2 axial ligand) By similarity
Metal bindingi188 – 1881Iron (heme B 2 axial ligand) By similarity
Metal bindingi206 – 2061Iron (heme B 1 axial ligand) By similarity

GO - Molecular functioni

  1. electron carrier activity Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
  3. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. anaerobic respiration Source: EcoCyc
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARV-MONOMER.
ECOL316407:JW1460-MONOMER.
MetaCyc:NARV-MONOMER.

Protein family/group databases

TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 2 gamma chain (EC:1.7.99.4)
Gene namesi
Name:narV
Ordered Locus Names:b1465, JW1460
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10644. narV.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 43Periplasmic By similarity
Transmembranei5 – 3026Helical; Name=1; By similarity
Add
BLAST
Topological domaini31 – 4818Cytoplasmic By similarity
Add
BLAST
Transmembranei49 – 7123Helical; Name=2; By similarity
Add
BLAST
Topological domaini72 – 8312Periplasmic By similarity
Add
BLAST
Transmembranei84 – 11330Helical; Name=3; By similarity
Add
BLAST
Topological domaini114 – 12512Cytoplasmic By similarity
Add
BLAST
Transmembranei126 – 14924Helical; Name=4; By similarity
Add
BLAST
Topological domaini150 – 18334Periplasmic By similarity
Add
BLAST
Transmembranei184 – 19916Helical; Name=5; By similarity
Add
BLAST
Topological domaini200 – 22627Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. nitrate reductase complex Source: InterPro
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Respiratory nitrate reductase 2 gamma chain
PRO_0000096731Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AF32.

Interactioni

Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

Protein-protein interaction databases

STRINGi511145.b1465.

Structurei

3D structure databases

ProteinModelPortaliP0AF32.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181.
HOGENOMiHOG000237376.
KOiK00374.
OMAiWTEAVGV.
OrthoDBiEOG6K13W6.
PhylomeDBiP0AF32.

Family and domain databases

InterProiIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamiPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMiSSF103501. SSF103501. 1 hit.
TIGRFAMsiTIGR00351. narI. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AF32-1 [UniParc]FASTAAdd to Basket

« Hide

MIQYLNVFFY DIYPYICATV FFLGSWLRYD YGQYTWRASS SQMLDKRGMV    50
IWSNLFHIGI LGIFFGHLFG MLTPHWMYAW FLPVAAKQLM AMVLGGICGV 100
LTLIGGAGLL WRRLTNQRVR ATSTTPDIII MSILLIQCLL GLSTIPFSAQ 150
YPDGSEMMKL VGWAQSIVTF RGGSSEMLNG VAFVFRLHLV LGMTIFLLFP 200
FTRLVHVWSA PFEYFTRRYQ IVRSRR 226
Length:226
Mass (Da):26,018
Last modified:December 20, 2005 - v1
Checksum:i1D5FAC307F3D5B84
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17110 Genomic DNA. Translation: CAA34967.1.
U00096 Genomic DNA. Translation: AAC74547.1.
AP009048 Genomic DNA. Translation: BAA15102.1.
PIRiS11430.
RefSeqiNP_415982.1. NC_000913.3.
YP_489730.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74547; AAC74547; b1465.
BAA15102; BAA15102; BAA15102.
GeneIDi12934343.
946029.
KEGGiecj:Y75_p1441.
eco:b1465.
PATRICi32118222. VBIEscCol129921_1531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17110 Genomic DNA. Translation: CAA34967.1 .
U00096 Genomic DNA. Translation: AAC74547.1 .
AP009048 Genomic DNA. Translation: BAA15102.1 .
PIRi S11430.
RefSeqi NP_415982.1. NC_000913.3.
YP_489730.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AF32.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1465.

Protein family/group databases

TCDBi 5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74547 ; AAC74547 ; b1465 .
BAA15102 ; BAA15102 ; BAA15102 .
GeneIDi 12934343.
946029.
KEGGi ecj:Y75_p1441.
eco:b1465.
PATRICi 32118222. VBIEscCol129921_1531.

Organism-specific databases

EchoBASEi EB0638.
EcoGenei EG10644. narV.

Phylogenomic databases

eggNOGi COG2181.
HOGENOMi HOG000237376.
KOi K00374.
OMAi WTEAVGV.
OrthoDBi EOG6K13W6.
PhylomeDBi P0AF32.

Enzyme and pathway databases

BioCyci EcoCyc:NARV-MONOMER.
ECOL316407:JW1460-MONOMER.
MetaCyc:NARV-MONOMER.

Miscellaneous databases

PROi P0AF32.

Gene expression databases

Genevestigatori P0AF32.

Family and domain databases

InterProi IPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view ]
Pfami PF02665. Nitrate_red_gam. 1 hit.
[Graphical view ]
SUPFAMi SSF103501. SSF103501. 1 hit.
TIGRFAMsi TIGR00351. narI. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
    Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
    Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNARV_ECOLI
AccessioniPrimary (citable) accession number: P0AF32
Secondary accession number(s): P19316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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