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Protein

Nitrate/nitrite response regulator protein NarL

Gene

narL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi173 – 192H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoCyc

GO - Biological processi

  • nitrate assimilation Source: UniProtKB-KW
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • regulation of gene expression Source: EcoCyc
  • regulation of nitrate assimilation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARL-MONOMER.
ECOL316407:JW1212-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite response regulator protein NarL
Gene namesi
Name:narL
Synonyms:frdR
Ordered Locus Names:b1221, JW1212
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10643. narL.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811451 – 216Nitrate/nitrite response regulator protein NarLAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AF28.
PaxDbiP0AF28.
PRIDEiP0AF28.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000880.

Interactioni

Protein-protein interaction databases

BioGridi4261632. 8 interactors.
DIPiDIP-35941N.
IntActiP0AF28. 4 interactors.
STRINGi511145.b1221.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi16 – 26Combined sources11
Beta strandi32 – 40Combined sources9
Helixi41 – 51Combined sources11
Beta strandi54 – 59Combined sources6
Helixi63 – 65Combined sources3
Helixi67 – 76Combined sources10
Beta strandi81 – 87Combined sources7
Helixi92 – 100Combined sources9
Beta strandi104 – 108Combined sources5
Helixi113 – 125Combined sources13
Turni132 – 134Combined sources3
Helixi135 – 141Combined sources7
Helixi153 – 155Combined sources3
Helixi158 – 167Combined sources10
Turni168 – 170Combined sources3
Helixi173 – 180Combined sources8
Helixi184 – 197Combined sources14
Helixi203 – 212Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A04X-ray2.20A/B2-216[»]
1JE8X-ray2.12A/B/E/F147-216[»]
1RNLX-ray2.40A2-216[»]
1ZG1X-ray2.30A/B/E/F147-216[»]
1ZG5X-ray2.30A/B/E/F147-216[»]
ProteinModelPortaliP0AF28.
SMRiP0AF28.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AF28.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 124Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini149 – 214HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105WH1. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AF28.
KOiK07684.
OMAiIRATFHG.
PhylomeDBiP0AF28.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQEPATIL LIDDHPMLRT GVKQLISMAP DITVVGEASN GEQGIELAES
60 70 80 90 100
LDPDLILLDL NMPGMNGLET LDKLREKSLS GRIVVFSVSN HEEDVVTALK
110 120 130 140 150
RGADGYLLKD MEPEDLLKAL HQAAAGEMVL SEALTPVLAA SLRANRATTE
160 170 180 190 200
RDVNQLTPRE RDILKLIAQG LPNKMIARRL DITESTVKVH VKHMLKKMKL
210
KSRVEAAVWV HQERIF
Length:216
Mass (Da):23,927
Last modified:December 20, 2005 - v1
Checksum:i7DED78E4EF7EC57D
GO

Sequence cautioni

The sequence CAA33023 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115D → H in AAA24199 (PubMed:2649492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13360 Genomic DNA. Translation: CAA31742.1.
M24910 Genomic DNA. Translation: AAA24199.1.
X14884 Genomic DNA. Translation: CAA33023.1. Different initiation.
X69189 Genomic DNA. Translation: CAA48935.1.
U00096 Genomic DNA. Translation: AAC74305.1.
AP009048 Genomic DNA. Translation: BAA36089.1.
PIRiS09285. RGECNL.
RefSeqiNP_415739.1. NC_000913.3.
WP_000070491.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74305; AAC74305; b1221.
BAA36089; BAA36089; BAA36089.
GeneIDi945795.
KEGGiecj:JW1212.
eco:b1221.
PATRICi32117700. VBIEscCol129921_1272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13360 Genomic DNA. Translation: CAA31742.1.
M24910 Genomic DNA. Translation: AAA24199.1.
X14884 Genomic DNA. Translation: CAA33023.1. Different initiation.
X69189 Genomic DNA. Translation: CAA48935.1.
U00096 Genomic DNA. Translation: AAC74305.1.
AP009048 Genomic DNA. Translation: BAA36089.1.
PIRiS09285. RGECNL.
RefSeqiNP_415739.1. NC_000913.3.
WP_000070491.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A04X-ray2.20A/B2-216[»]
1JE8X-ray2.12A/B/E/F147-216[»]
1RNLX-ray2.40A2-216[»]
1ZG1X-ray2.30A/B/E/F147-216[»]
1ZG5X-ray2.30A/B/E/F147-216[»]
ProteinModelPortaliP0AF28.
SMRiP0AF28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261632. 8 interactors.
DIPiDIP-35941N.
IntActiP0AF28. 4 interactors.
STRINGi511145.b1221.

Proteomic databases

EPDiP0AF28.
PaxDbiP0AF28.
PRIDEiP0AF28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74305; AAC74305; b1221.
BAA36089; BAA36089; BAA36089.
GeneIDi945795.
KEGGiecj:JW1212.
eco:b1221.
PATRICi32117700. VBIEscCol129921_1272.

Organism-specific databases

EchoBASEiEB0637.
EcoGeneiEG10643. narL.

Phylogenomic databases

eggNOGiENOG4105WH1. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AF28.
KOiK07684.
OMAiIRATFHG.
PhylomeDBiP0AF28.

Enzyme and pathway databases

BioCyciEcoCyc:NARL-MONOMER.
ECOL316407:JW1212-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AF28.
PROiP0AF28.

Gene expression databases

CollecTFiEXPREG_00000880.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNARL_ECOLI
AccessioniPrimary (citable) accession number: P0AF28
Secondary accession number(s): P10957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.