Reviewed, UniProtKB/Swiss-Prot P0AF26 (NARJ_ECOLI)

Names and origin

Protein names

Recommended name:
    Respiratory nitrate reductase 1 delta chain
    EC=1.7.99.4

Gene names

Name:	narJ
Ordered Locus Names:	b1226, JW1217

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the assembly of the nitrate reductase-cytochrome b-NR complex to be fully active in the membrane. Ref.6
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.

Ontologies

Keywords
Biological process	Nitrate assimilation
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nitrate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	nitrate reductase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
narG	P09152	1	EBI-555043,EBI-547248
sdhA	P0AC41	1	EBI-555043,EBI-371263

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Respiratory nitrate reductase 1 delta chain		PRO_0000096725

Sequences

	Sequence		Length	Mass (Da)
	P0AF26-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 17306C34A57DF1E2	FASTA	236	26,449
	10 20 30 40 50 60 MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA 70 80 90 100 110 120 QYSELFDRGR ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY 130 140 150 160 170 180 LAQLPQSEAV EGLKDIAPIL ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD 190 200 210 220 230 EARDDTPQAL DAVWEEEQVK FFADKGCGDS AITAHQRRFA GAVAPQYLNI TTGGQH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes." Sodergren E.J., Demoss J.A. J. Bacteriol. 170:1721-1729(1988) [PubMed: 2832376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer." Blasco F., Iobbi C., Giordano G., Chippaux M., Bonnefoy V. Mol. Gen. Genet. 218:249-256(1989) [PubMed: 2674654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50. Strain: K12 / TG1.
[6]	"The narJ gene product is required for biogenesis of respiratory nitrate reductase in Escherichia coli." Dubourdieu M., Demoss J.A. J. Bacteriol. 174:867-872(1992) [PubMed: 1732220] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20147 Genomic DNA. Translation: AAA24196.1. U00096 Genomic DNA. Translation: AAC74310.1. AP009048 Genomic DNA. Translation: BAA36096.1. X16181 Genomic DNA. Translation: CAA34305.1.
PIR	BVECJ. B27737.
RefSeq	AP_001854.1. NP_415744.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AF26. 10 interactions.
Genome annotation databases
GeneID	945807.
GenomeReviews	Gene locus JW1217 in contig AP009048_GR. Gene locus b1226 in contig U00096_GR.
KEGG	ecj:JW1217. eco:b1226.
Organism-specific databases
EchoBASE	EB0635.
EcoGene	EG10641. narJ.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AF26.
OMA	P0AF26. YLNISNG.
Enzyme and pathway databases
BioCyc	EcoCyc:NARJ-MON.
Family and domain databases
InterPro	IPR003765. Nitrate_red_del. [Graphical view]
Pfam	PF02613. Nitrate_red_del. 1 hit. [Graphical view]
TIGRFAMs	TIGR00684. narJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NARJ_ECOLI

Accession

Primary (citable) accession number: P0AF26
Secondary accession number(s): P11351

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)