P0AF20 (NAGC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylglucosamine repressor

Gene names

Name:	nagC
Synonyms:	nagR
Ordered Locus Names:	b0676, JW0662

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a repressor of the nagEBACD operon and acts both as an activator and a repressor for the transcription of the glmSU operon. Ref.6
Induction	By N-acetylglucosamine.
Sequence similarities	Belongs to the ROK (nagC/xylR) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	N-acetylglucosamine repressor		PRO_0000095691
Regions
	DNA binding	35 – 44	10	H-T-H motif By similarity
Experimental info
	Sequence conflict	203 – 204	2	EH → DD in CAA32354. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AF20 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 28C4C85D736627C8	FASTA	406	44,541
	10 20 30 40 50 60 MTPGGQAQIG NVDLVKQLNS AAVYRLIDQY GPISRIQIAE QSQLAPASVT KITRQLIERG 70 80 90 100 110 120 LIKEVDQQAS TGGRRAISIV TETRNFHAIG VRLGRHDATI TLFDLSSKVL AEEHYPLPER 130 140 150 160 170 180 TQQTLEHALL NAIAQFIDSY QRKLRELIAI SVILPGLVDP DSGKIHYMPH IQVENWGLVE 190 200 210 220 230 240 ALEERFKVTC FVGHDIRSLA LAEHYFGASQ DCEDSILVRV HRGTGAGIIS NGRIFIGRNG 250 260 270 280 290 300 NVGEIGHIQV EPLGERCHCG NFGCLETIAA NAAIEQRVLN LLKQGYQSRV PLDDCTIKTI 310 320 330 340 350 360 CKAANKGDSL ASEVIEYVGR HLGKTIAIAI NLFNPQKIVI AGEITEADKV LLPAIESCIN 370 380 390 400 TQALKAFRTN LPVVRSELDH RSAIGAFALV KRAMLNGILL QHLLEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon." Plumbridge J. Mol. Microbiol. 3:505-515(1989) [PubMed: 2668691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli." Peri K.G., Goldie H., Waygood E.B. Biochem. Cell Biol. 68:123-137(1990) [PubMed: 2190615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Co-ordinated regulation of amino sugar biosynthesis and degradation: the NagC repressor acts as both an activator and a repressor for the transcription of the glmUS operon and requires two separated NagC binding sites." Plumbridge J. EMBO J. 14:3958-3965(1995) [PubMed: 7545108] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14135 Genomic DNA. Translation: CAA32354.1. AF052007 Genomic DNA. Translation: AAC09326.1. U00096 Genomic DNA. Translation: AAC73770.1. AP009048 Genomic DNA. Translation: BAA35319.1.
PIR	C64802.
RefSeq	NP_415202.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AF20.
SMR	P0AF20. Positions 14-405.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35984N.
IntAct	P0AF20. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001646; EBESCP00000001646; EBESCG00000001357. EBESCT00000017332; EBESCP00000016623; EBESCG00000016388.
GeneID	945285.
GenomeReviews	Gene locus JW0662 in contig AP009048_GR. Gene locus b0676 in contig U00096_GR.
KEGG	ecj:JW0662. eco:b0676.
PATRIC	32116537. VBIEscCol129921_0701.
Organism-specific databases
EchoBASE	EB0630.
EcoGene	EG10636. nagC.
Phylogenomic databases
eggNOG	COG1940.
GeneTree	EBGT00050000009665.
HOGENOM	HBG297939.
OMA	MTTGGQA.
PhylomeDB	P0AF20.
ProtClustDB	CLSK870201.
Enzyme and pathway databases
BioCyc	EcoCyc:PD00266.
Gene expression databases
Genevestigator	P0AF20.
Family and domain databases
InterPro	IPR000600. ROK. IPR000835. Tscrpt_reg_HTH_MarR-typ. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K02565.
Pfam	PF01047. MarR. 1 hit. PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGC_ECOLI

Accession

Primary (citable) accession number: P0AF20
Secondary accession number(s): P15301

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program