Reviewed, UniProtKB/Swiss-Prot P0AF19 (NAGA_ECO57)

Names and origin

Protein names

Recommended name:
    N-acetylglucosamine-6-phosphate deacetylase
    EC=3.5.1.25
Alternative name(s):
    GlcNAc 6-P deacetylase

Gene names

Name:	nagA
Ordered Locus Names:	Z0824, ECs0707

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate.
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 4/5.
Sequence similarities	Belongs to the nagA family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetylglucosamine-6-phosphate deacetylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	N-acetylglucosamine-6-phosphate deacetylase		PRO_0000170916

Sequences

	Sequence		Length	Mass (Da)
	P0AF19-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: A10B015ADFC98FCA	FASTA	382	40,949
	10 20 30 40 50 60 MYALTQGRIF TGHEFLDDHA VVIADGLIKS VCPVAELPPE IEQRSLNGAI LSPGFIDVQL 70 80 90 100 110 120 NGCGGVQFND TAEAVSVETL EIMQKANEKS GCTNYLPTLI TTSDELMKQG VRVMREYLAK 130 140 150 160 170 180 HPNQALGLHL EGPWLNLVKK GTHNPNFVRK PDAALVDFLC ENADVITKVT LAPEMVPAEV 190 200 210 220 230 240 ISKLANAGIV VSAGHSNATL KEAKAGFRAG ITFATHLYNA MPYITGREPG LAGAILDEAD 250 260 270 280 290 300 IYCGIIADGL HVDYANIRNA KRLKGDKLCL VTDATAPAGA NIEQFIFAGK TIYYRNGLCV 310 320 330 340 350 360 DENGTLSGSS LTMIEGVRNL VEHCGIALDE VLRMATLYPA RAIGVEKRLG TLAAGKVANL 370 380 TAFTPDFKIT KTIVNGNEVV TQ

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG54999.1. BA000007 Genomic DNA. Translation: BAB34130.1.
PIR	C85567. C90717.
RefSeq	NP_286391.1. NP_308734.1.
3D structure databases
SMR	P0AF19. Positions 1-382.
ModBase	Search...
Genome annotation databases
GeneID	917076. 957746.
GenomeReviews	Gene locus Z0824 in contig AE005174_GR. Gene locus ECs0707 in contig BA000007_GR.
KEGG	ece:Z0824. ecs:ECs0707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AF19.
OMA	ETMQIMH.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0707-MON.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. IPR003764. GlcNAc_6-P_deAcase. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00221. nagA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NAGA_ECO57

Accession

Primary (citable) accession number: P0AF19
Secondary accession number(s): P15300

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)