N-acetylglucosamine-6-phosphate deacetylase - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0AF18 (NAGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
N-acetylglucosamine-6-phosphate deacetylase
EC=3.5.1.25

Alternative name(s):
GlcNAc 6-P deacetylase

Gene names

Name:	nagA
Ordered Locus Names:	b0677, JW0663

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-acetyl-D-glucosamine 6-phosphate + H₂O = D-glucosamine 6-phosphate + acetate.
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 4/5.
Induction	By N-acetylglucosamine. Induced by low extracellular levels of magnesium via the phoQ/phoP two-component regulatory system. Ref.6
Sequence similarities	Belongs to the nagA family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	N-acetylglucosamine-6-phosphate deacetylase activity Inferred from direct assay. Source: EcoCyc zinc ion binding Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 382

382

N-acetylglucosamine-6-phosphate deacetylase

PRO_0000170915

Secondary structure

382

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AF18 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: A10B015ADFC98FCA
Show »

FASTA

382

40,949

        10         20         30         40         50         60 
MYALTQGRIF TGHEFLDDHA VVIADGLIKS VCPVAELPPE IEQRSLNGAI LSPGFIDVQL 

        70         80         90        100        110        120 
NGCGGVQFND TAEAVSVETL EIMQKANEKS GCTNYLPTLI TTSDELMKQG VRVMREYLAK 

       130        140        150        160        170        180 
HPNQALGLHL EGPWLNLVKK GTHNPNFVRK PDAALVDFLC ENADVITKVT LAPEMVPAEV 

       190        200        210        220        230        240 
ISKLANAGIV VSAGHSNATL KEAKAGFRAG ITFATHLYNA MPYITGREPG LAGAILDEAD 

       250        260        270        280        290        300 
IYCGIIADGL HVDYANIRNA KRLKGDKLCL VTDATAPAGA NIEQFIFAGK TIYYRNGLCV 

       310        320        330        340        350        360 
DENGTLSGSS LTMIEGVRNL VEHCGIALDE VLRMATLYPA RAIGVEKRLG TLAAGKVANL 

       370        380 
TAFTPDFKIT KTIVNGNEVV TQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon." Plumbridge J. Mol. Microbiol. 3:505-515(1989) [PubMed: 2668691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli." Peri K.G., Goldie H., Waygood E.B. Biochem. Cell Biol. 68:123-137(1990) [PubMed: 2190615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R. J. Bacteriol. 185:3696-3702(2003) [PubMed: 12813061] [Abstract] Cited for: INDUCTION. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14135 Genomic DNA. Translation: CAA32353.1.
AF052007 Genomic DNA. Translation: AAC09325.1.
U00096 Genomic DNA. Translation: AAC73771.1.
AP009048 Genomic DNA. Translation: BAA35320.1.

PIR

A37018.

RefSeq

NP_415203.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YMY	X-ray	2.60	A/B	1-382	[»]
1YRR	X-ray	2.00	A/B	1-382	[»]
2P50	X-ray	2.20	A/B/C/D	1-382	[»]
2P53	X-ray	2.10	A/B	1-382	[»]

ProteinModelPortal

P0AF18.

SMR

P0AF18. Positions 1-382.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AF18. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000144; EBESCP00000000144; EBESCG00000000114.
EBESCT00000000145; EBESCP00000000145; EBESCG00000000114.
EBESCT00000017174; EBESCP00000016465; EBESCG00000016233.

GeneID

945289.

GenomeReviews

Gene locus JW0663 in contig AP009048_GR.
Gene locus b0677 in contig U00096_GR.

KEGG

ecj:JW0663.
eco:b0677.

PATRIC

32116539. VBIEscCol129921_0702.

Organism-specific databases

EchoBASE

EB0626.

EcoGene

EG10632. nagA.

Phylogenomic databases

eggNOG

COG1820.

GeneTree

EBGT00050000009830.

HOGENOM

HBG464950.

OMA

TSSDEDM.

PhylomeDB

P0AF18.

ProtClustDB

PRK11170.

Enzyme and pathway databases

BioCyc

EcoCyc:NAG6PDEACET-MONOMER.
MetaCyc:NAG6PDEACET-MONOMER.

Gene expression databases

Genevestigator

P0AF18.

Family and domain databases

InterPro

IPR006680. Amidohydro_1.
IPR003764. GlcNAc_6-P_deAcase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]

K01443.

Pfam

PF01979. Amidohydro_1. 1 hit.
[Graphical view]

SUPFAM

SSF51338. Metalo_hydrolase. 2 hits.

TIGRFAMs

TIGR00221. NagA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NAGA_ECOLI

Accession

Primary (citable) accession number: P0AF18
Secondary accession number(s): P15300

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents