P0AF16 (MURJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable peptidoglycan biosynthesis protein MurJ

Gene names

Name:	murJ
Synonyms:	mviN, yceN
Ordered Locus Names:	b1069, JW1056

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in peptidoglycan biosynthesis. Ref.5 Ref.6 Ref.7
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.4.
Sequence similarities	Belongs to the MurJ/MviN family.
Caution	Was originally (Ref.6) thought to be a lipid II flippase. It was shown later that it has no direct role in lipid II translocation (Ref.7).

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Probable peptidoglycan biosynthesis protein MurJ		PRO_0000182006
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	271 – 291	21	Helical; Potential
	Transmembrane	314 – 334	21	Helical; Potential
	Transmembrane	354 – 374	21	Helical; Potential
	Transmembrane	383 – 403	21	Helical; Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Transmembrane	443 – 463	21	Helical; Potential
	Transmembrane	481 – 501	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AF16 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: CB20FE3CFC9419C2	FASTA	511	55,267
	10 20 30 40 50 60 MNLLKSLAAV SSMTMFSRVL GFARDAIVAR IFGAGMATDA FFVAFKLPNL LRRIFAEGAF 70 80 90 100 110 120 SQAFVPILAE YKSKQGEDAT RVFVSYVSGL LTLALAVVTV AGMLAAPWVI MVTAPGFADT 130 140 150 160 170 180 ADKFALTSQL LKITFPYILL ISLASLVGAI LNTWNRFSIP AFAPTLLNIS MIGFALFAAP 190 200 210 220 230 240 YFNPPVLALA WAVTVGGVLQ LVYQLPHLKK IGMLVLPRIN FHDAGAMRVV KQMGPAILGV 250 260 270 280 290 300 SVSQISLIIN TIFASFLASG SVSWMYYADR LMEFPSGVLG VALGTILLPS LSKSFASGNH 310 320 330 340 350 360 DEYNRLMDWG LRLCFLLALP SAVALGILSG PLTVSLFQYG KFTAFDALMT QRALIAYSVG 370 380 390 400 410 420 LIGLIVVKVL APGFYSRQDI KTPVKIAIVT LILTQLMNLA FIGPLKHAGL SLSIGLAACL 430 440 450 460 470 480 NASLLYWQLR KQKIFTPQPG WMAFLLRLVV AVLVMSGVLL GMLHIMPEWS LGTMPWRLLR 490 500 510 LMAVVLAGIA AYFAALAVLG FKVKEFARRT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli." Inoue A., Murata Y., Takahashi H., Tsuji N., Fujisaki S., Kato J. J. Bacteriol. 190:7298-7301(2008) [PubMed: 18708495] [Abstract] Cited for: FUNCTION IN PEPTIDOGLYCAN BIOSYNTHESIS.
[6]	"Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli." Ruiz N. Proc. Natl. Acad. Sci. U.S.A. 105:15553-15557(2008) [PubMed: 18832143] [Abstract] Cited for: FUNCTION IN PEPTIDOGLYCAN BIOSYNTHESIS, PROPOSED FUNCTION IN LIPID II TRANSLOCATION, GENE NAME. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[7]	"Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane." Mohammadi T., van Dam V., Sijbrandi R., Vernet T., Zapun A., Bouhss A., Diepeveen-de Bruin M., Nguyen-Disteche M., de Kruijff B., Breukink E. EMBO J. 30:1425-1432(2011) [PubMed: 21386816] [Abstract] Cited for: SHOWS THAT IT HAS NO DIRECT ROLE IN LIPID II TRANSLOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74153.1. AP009048 Genomic DNA. Translation: BAA35877.1.
PIR	B64850.
RefSeq	NP_415587.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AF16.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AF16. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002703; EBESCP00000002703; EBESCG00000002204. EBESCT00000016102; EBESCP00000015393; EBESCG00000015162.
GeneID	945487.
GenomeReviews	Gene locus JW1056 in contig AP009048_GR. Gene locus b1069 in contig U00096_GR.
KEGG	ecj:JW1056. eco:b1069.
PATRIC	32117377. VBIEscCol129921_1111.
Organism-specific databases
EchoBASE	EB3639.
EcoGene	EG13880. murJ.
Phylogenomic databases
eggNOG	COG0728.
GeneTree	EBGT00050000009661.
HOGENOM	HBG731814.
OMA	FISQSIA.
PhylomeDB	P0AF16.
ProtClustDB	CLSK868744.
Enzyme and pathway databases
BioCyc	EcoCyc:G6561-MONOMER.
Gene expression databases
Genevestigator	P0AF16.
Family and domain databases
InterPro	IPR004268. Flagellin_assembly_MviN. [Graphical view]
KO	K03980.
Pfam	PF03023. MVIN. 1 hit. [Graphical view]
PRINTS	PR01806. VIRFACTRMVIN.
TIGRFAMs	TIGR01695. MviN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURJ_ECOLI

Accession

Primary (citable) accession number: P0AF16
Secondary accession number(s): P75932

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program