Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Gene

mtnN

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.UniRule annotation
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN), 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN), 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12Proton acceptorUniRule annotation1
Binding sitei78Substrate; via amide nitrogenUniRule annotation1
Binding sitei152Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei197Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00904; UER00871

Names & Taxonomyi

Protein namesi
Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseUniRule annotation (EC:3.2.2.9UniRule annotation)
Short name:
MTA/SAH nucleosidaseUniRule annotation
Short name:
MTANUniRule annotation
Alternative name(s):
5'-methylthioadenosine nucleosidaseUniRule annotation
Short name:
MTA nucleosidaseUniRule annotation
S-adenosylhomocysteine nucleosidaseUniRule annotation
Short name:
AdoHcy nucleosidaseUniRule annotation
Short name:
SAH nucleosidaseUniRule annotation
Short name:
SRH nucleosidaseUniRule annotation
Gene namesi
Name:mtnNUniRule annotation
Ordered Locus Names:SF0151, S0154
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001644491 – 2325'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseAdd BLAST232

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP0AF15
SMRiP0AF15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 174Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUF Bacteria
COG0775 LUCA
HOGENOMiHOG000259346
KOiK01243
OMAiDQFVHSK

Family and domain databases

HAMAPiMF_01684 Salvage_MtnN, 1 hit
InterProiView protein in InterPro
IPR010049 MTA_SAH_Nsdase
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01704 MTA/SAH-Nsdase, 1 hit

Sequencei

Sequence statusi: Complete.

P0AF15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIIGAME EEVTLLRDKI ENRQTISLGG CEIYTGQLNG TEVALLKSGI
60 70 80 90 100
GKVAAALGAT LLLEHCKPDV IINTGSAGGL APTLKVGDIV VSDEARYHDA
110 120 130 140 150
DVTAFGYEYG QLPGCPAGFK ADDKLIAAAE ACIAELNLNA VRGLIVSGDA
160 170 180 190 200
FINGSVGLAK IRHNFPQAIA VEMEATAIAH VCHNFNVPFV VVRAISDVAD
210 220 230
QQSHLSFDEF LAVAAKQSSL MVESLVQKLA HG
Length:232
Mass (Da):24,354
Last modified:December 20, 2005 - v1
Checksum:i9B1FF9BEC39D4F2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN41814.1
AE014073 Genomic DNA Translation: AAP15695.1
RefSeqiNP_706107.1, NC_004337.2
WP_000689844.1, NZ_NIYV01000058.1

Genome annotation databases

EnsemblBacteriaiAAN41814; AAN41814; SF0151
AAP15695; AAP15695; S0154
GeneIDi1024491
KEGGisfl:SF0151
sfx:S0154
PATRICifig|198214.7.peg.169

Similar proteinsi

Entry informationi

Entry nameiMTNN_SHIFL
AccessioniPrimary (citable) accession number: P0AF15
Secondary accession number(s): P24247
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health