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Reviewed, UniProtKB/Swiss-Prot P0AF14 (MTNN_ECO57)

Last modified November 3, 2009. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
      Short name=MTA/SAH nucleosidase
      Short name=MTAN
    EC=3.2.2.9
Alternative name(s):
    5'-methylthioadenosine nucleosidase
      Short name=MTA nucleosidase
    S-adenosylhomocysteine nucleosidase
      Short name=SAH nucleosidase
      Short name=AdoHcy nucleosidase
      Short name=SRH nucleosidase
Gene names
Name: mtnN
Ordered Locus Names: Z0170, ECs0163
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity.

Catalytic activity

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684

S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2322325'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
PRO_0000164440

Regions

Region173 – 1742Substrate binding By similarity

Sites

Active site121Proton acceptor By similarity
Binding site781Substrate; via amide nitrogen By similarity
Binding site1521Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1971Substrate By similarity

Secondary structure

1 ............................... 232
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P0AF14-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 9B1FF9BEC39D4F2C

FASTA23224,354
        10         20         30         40         50         60 
MKIGIIGAME EEVTLLRDKI ENRQTISLGG CEIYTGQLNG TEVALLKSGI GKVAAALGAT 

        70         80         90        100        110        120 
LLLEHCKPDV IINTGSAGGL APTLKVGDIV VSDEARYHDA DVTAFGYEYG QLPGCPAGFK 

       130        140        150        160        170        180 
ADDKLIAAAE ACIAELNLNA VRGLIVSGDA FINGSVGLAK IRHNFPQAIA VEMEATAIAH 

       190        200        210        220        230 
VCHNFNVPFV VVRAISDVAD QQSHLSFDEF LAVAAKQSSL MVESLVQKLA HG

« Hide

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Cross-references

Sequence databases

AE005174 Genomic DNA. Translation: AAG54463.1.
BA000007 Genomic DNA. Translation: BAB33586.1.
PIRC85500.
C90649.
RefSeqNP_285855.1.
NP_308190.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3DF9X-ray1.95A/B1-232[»]
SMRP0AF14. Positions 1-232.
ModBaseSearch...

Genome annotation databases

GeneID913817.
956877.
GenomeReviewsGene locus Z0170 in contig AE005174_GR.
Gene locus ECs0163 in contig BA000007_GR.
KEGGece:Z0170.
ecs:ECs0163.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP0AF14.
OMAAQVCHQF.

Enzyme and pathway databases

BioCycECOL83334:ECS0163-MON.

Family and domain databases

HAMAPMF_01684.
[Tree]
InterProIPR010049. MTA_SAH_Nsdase.
IPR000845. Nucleoside_phosphorylase.
IPR018017. Nucleoside_phosphorylase_1.
[Graphical view]
PANTHERPTHR21234. PNP_UDP. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMTNN_ECO57
AccessionPrimary (citable) accession number: P0AF14
Secondary accession number(s): P24247
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents