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P0AF06

- MOTB_ECOLI

UniProt

P0AF06 - MOTB_ECOLI

Protein

Motility protein B

Gene

motB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

    GO - Biological processi

    1. archaeal or bacterial-type flagellum-dependent cell motility Source: UniProtKB-KW
    2. chemotaxis Source: UniProtKB-KW

    Keywords - Biological processi

    Chemotaxis, Flagellar rotation

    Enzyme and pathway databases

    BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
    ECOL316407:JW1878-MONOMER.

    Protein family/group databases

    TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Motility protein B
    Alternative name(s):
    Chemotaxis protein MotB
    Gene namesi
    Name:motB
    Ordered Locus Names:b1889, JW1878
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10602. motB.

    Subcellular locationi

    Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication
    Note: The OmpA-like domain probably functions to anchor the complex to the cell wall.

    GO - Cellular componenti

    1. integral component of membrane Source: EcoliWiki
    2. plasma membrane Source: EcoliWiki

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi31 – 311A → T: Complete loss of motility.
    Mutagenesisi32 – 321D → N: Complete loss of motility. 1 Publication
    Mutagenesisi39 – 391A → V: Complete loss of motility. 1 Publication
    Mutagenesisi159 – 1591P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
    Mutagenesisi164 – 1641G → D: Complete loss of motility. 1 Publication
    Mutagenesisi196 – 1961T → I: Complete loss of motility. 1 Publication
    Mutagenesisi197 – 1971D → N: Complete loss of motility. 1 Publication
    Mutagenesisi205 – 2051E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication
    Mutagenesisi214 – 2141S → F: Complete loss of motility. 1 Publication
    Mutagenesisi217 – 2171R → W: Complete loss of motility. 1 Publication
    Mutagenesisi222 – 2221R → H: Complete loss of motility. 1 Publication
    Mutagenesisi240 – 2401G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
    Mutagenesisi242 – 2421A → T or V: Complete loss of motility. 1 Publication
    Mutagenesisi258 – 2581R → C or H: Complete loss of motility. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Motility protein BPRO_0000189585Add
    BLAST

    Proteomic databases

    PaxDbiP0AF06.
    PRIDEiP0AF06.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AF06.

    Interactioni

    Subunit structurei

    Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.

    Protein-protein interaction databases

    DIPiDIP-47996N.
    IntActiP0AF06. 4 interactions.
    STRINGi511145.b1889.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AF06.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2727Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini50 – 308259Periplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 4922Helical; Signal-anchor for type II membrane proteinCuratedAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 268121OmpA-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MotB family.Curated
    Contains 1 OmpA-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1360.
    HOGENOMiHOG000254689.
    KOiK02557.
    OMAiQDGLRIQ.
    OrthoDBiEOG6VQPR8.
    PhylomeDBiP0AF06.

    Family and domain databases

    Gene3Di3.30.1330.60. 1 hit.
    InterProiIPR025713. MotB_N_dom.
    IPR006665. OmpA/MotB_C.
    [Graphical view]
    PfamiPF13677. MotB_plug. 1 hit.
    PF00691. OmpA. 1 hit.
    [Graphical view]
    SUPFAMiSSF103088. SSF103088. 1 hit.
    PROSITEiPS51123. OMPA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AF06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS    50
    SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ 100
    PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI 150
    QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP 200
    YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR 250
    GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP 300
    TMPSAEPR 308
    Length:308
    Mass (Da):34,186
    Last modified:December 20, 2005 - v1
    Checksum:i2F20C551C44BD6E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01652 Genomic DNA. Translation: AAA24178.1.
    U00096 Genomic DNA. Translation: AAC74959.1.
    AP009048 Genomic DNA. Translation: BAA15710.1.
    M34669 Genomic DNA. Translation: AAA23572.1.
    PIRiA64952. QRECMB.
    RefSeqiNP_416403.1. NC_000913.3.
    YP_490151.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74959; AAC74959; b1889.
    BAA15710; BAA15710; BAA15710.
    GeneIDi12932041.
    946402.
    KEGGiecj:Y75_p1865.
    eco:b1889.
    PATRICi32119105. VBIEscCol129921_1970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01652 Genomic DNA. Translation: AAA24178.1 .
    U00096 Genomic DNA. Translation: AAC74959.1 .
    AP009048 Genomic DNA. Translation: BAA15710.1 .
    M34669 Genomic DNA. Translation: AAA23572.1 .
    PIRi A64952. QRECMB.
    RefSeqi NP_416403.1. NC_000913.3.
    YP_490151.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AF06.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47996N.
    IntActi P0AF06. 4 interactions.
    STRINGi 511145.b1889.

    Protein family/group databases

    TCDBi 1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

    Proteomic databases

    PaxDbi P0AF06.
    PRIDEi P0AF06.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74959 ; AAC74959 ; b1889 .
    BAA15710 ; BAA15710 ; BAA15710 .
    GeneIDi 12932041.
    946402.
    KEGGi ecj:Y75_p1865.
    eco:b1889.
    PATRICi 32119105. VBIEscCol129921_1970.

    Organism-specific databases

    EchoBASEi EB0597.
    EcoGenei EG10602. motB.

    Phylogenomic databases

    eggNOGi COG1360.
    HOGENOMi HOG000254689.
    KOi K02557.
    OMAi QDGLRIQ.
    OrthoDBi EOG6VQPR8.
    PhylomeDBi P0AF06.

    Enzyme and pathway databases

    BioCyci EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
    ECOL316407:JW1878-MONOMER.

    Miscellaneous databases

    PROi P0AF06.

    Gene expression databases

    Genevestigatori P0AF06.

    Family and domain databases

    Gene3Di 3.30.1330.60. 1 hit.
    InterProi IPR025713. MotB_N_dom.
    IPR006665. OmpA/MotB_C.
    [Graphical view ]
    Pfami PF13677. MotB_plug. 1 hit.
    PF00691. OmpA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103088. SSF103088. 1 hit.
    PROSITEi PS51123. OMPA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane."
      Stader J., Matsumura P., Vacante D., Dean G.E., Macnab R.M.
      J. Bacteriol. 166:244-252(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Tandem translation starts in the cheA locus of Escherichia coli."
      Kofoid E.C., Parkinson J.S.
      J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-308.
    6. "Bacterial motility: membrane topology of the Escherichia coli MotB protein."
      Chun S.Y., Parkinson J.S.
      Science 239:276-278(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TOPOLOGY, SUGGESTION OF ROLE AS A CELL-WALL ANCHOR.
    7. "Mutant MotB proteins in Escherichia coli."
      Blair D.F., Kim D.Y., Berg H.C.
      J. Bacteriol. 173:4049-4055(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-32; ALA-39; PRO-159; GLY-164; THE-196; ASP-197; GLU-205; SER-214; ARG-217; ARG-222; GLY-240; ALA-242 AND ARG-258.
      Strain: K12 / RP437.
    8. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
      Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
      Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / RP3098.
    9. "Flagellar motility in bacteria structure and function of flagellar motor."
      Terashima H., Kojima S., Homma M.
      Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiMOTB_ECOLI
    AccessioniPrimary (citable) accession number: P0AF06
    Secondary accession number(s): P09349
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3