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Protein

Motility protein B

Gene

motB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Motility protein B
Alternative name(s):
Chemotaxis protein MotB
Gene namesi
Name:motB
Ordered Locus Names:b1889, JW1878
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10602. motB.

Subcellular locationi

  • Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication

  • Note: The OmpA-like domain probably functions to anchor the complex to the cell wall.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27Cytoplasmic1 PublicationAdd BLAST27
Transmembranei28 – 49Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST22
Topological domaini50 – 308Periplasmic1 PublicationAdd BLAST259

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31A → T: Complete loss of motility. 1
Mutagenesisi32D → N: Complete loss of motility. 1 Publication1
Mutagenesisi39A → V: Complete loss of motility. 1 Publication1
Mutagenesisi159P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication1
Mutagenesisi164G → D: Complete loss of motility. 1 Publication1
Mutagenesisi196T → I: Complete loss of motility. 1 Publication1
Mutagenesisi197D → N: Complete loss of motility. 1 Publication1
Mutagenesisi205E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication1
Mutagenesisi214S → F: Complete loss of motility. 1 Publication1
Mutagenesisi217R → W: Complete loss of motility. 1 Publication1
Mutagenesisi222R → H: Complete loss of motility. 1 Publication1
Mutagenesisi240G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication1
Mutagenesisi242A → T or V: Complete loss of motility. 1 Publication1
Mutagenesisi258R → C or H: Complete loss of motility. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001895851 – 308Motility protein BAdd BLAST308

Proteomic databases

PaxDbiP0AF06.
PRIDEiP0AF06.

Interactioni

Subunit structurei

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.

Protein-protein interaction databases

BioGridi4261035. 408 interactors.
DIPiDIP-47996N.
IntActiP0AF06. 4 interactors.
STRINGi511145.b1889.

Structurei

3D structure databases

ProteinModelPortaliP0AF06.
SMRiP0AF06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 268OmpA-likePROSITE-ProRule annotationAdd BLAST121

Sequence similaritiesi

Belongs to the MotB family.Curated
Contains 1 OmpA-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ER2. Bacteria.
COG1360. LUCA.
HOGENOMiHOG000254689.
InParanoidiP0AF06.
KOiK02557.
OMAiTIKAVPN.
PhylomeDBiP0AF06.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di3.30.1330.60. 1 hit.
InterProiIPR025713. MotB_N_dom.
IPR006665. OmpA-like.
[Graphical view]
PfamiPF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view]
SUPFAMiSSF103088. SSF103088. 1 hit.
PROSITEiPS51123. OMPA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS
60 70 80 90 100
SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ
110 120 130 140 150
PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI
160 170 180 190 200
QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP
210 220 230 240 250
YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR
260 270 280 290 300
GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP

TMPSAEPR
Length:308
Mass (Da):34,186
Last modified:December 20, 2005 - v1
Checksum:i2F20C551C44BD6E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1.
U00096 Genomic DNA. Translation: AAC74959.1.
AP009048 Genomic DNA. Translation: BAA15710.1.
M34669 Genomic DNA. Translation: AAA23572.1.
PIRiA64952. QRECMB.
RefSeqiNP_416403.1. NC_000913.3.
WP_000795630.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74959; AAC74959; b1889.
BAA15710; BAA15710; BAA15710.
GeneIDi946402.
KEGGiecj:JW1878.
eco:b1889.
PATRICi32119105. VBIEscCol129921_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1.
U00096 Genomic DNA. Translation: AAC74959.1.
AP009048 Genomic DNA. Translation: BAA15710.1.
M34669 Genomic DNA. Translation: AAA23572.1.
PIRiA64952. QRECMB.
RefSeqiNP_416403.1. NC_000913.3.
WP_000795630.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AF06.
SMRiP0AF06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261035. 408 interactors.
DIPiDIP-47996N.
IntActiP0AF06. 4 interactors.
STRINGi511145.b1889.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Proteomic databases

PaxDbiP0AF06.
PRIDEiP0AF06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74959; AAC74959; b1889.
BAA15710; BAA15710; BAA15710.
GeneIDi946402.
KEGGiecj:JW1878.
eco:b1889.
PATRICi32119105. VBIEscCol129921_1970.

Organism-specific databases

EchoBASEiEB0597.
EcoGeneiEG10602. motB.

Phylogenomic databases

eggNOGiENOG4105ER2. Bacteria.
COG1360. LUCA.
HOGENOMiHOG000254689.
InParanoidiP0AF06.
KOiK02557.
OMAiTIKAVPN.
PhylomeDBiP0AF06.

Enzyme and pathway databases

BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Miscellaneous databases

PROiP0AF06.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di3.30.1330.60. 1 hit.
InterProiIPR025713. MotB_N_dom.
IPR006665. OmpA-like.
[Graphical view]
PfamiPF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view]
SUPFAMiSSF103088. SSF103088. 1 hit.
PROSITEiPS51123. OMPA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AF06
Secondary accession number(s): P09349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.