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P0AF06

- MOTB_ECOLI

UniProt

P0AF06 - MOTB_ECOLI

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Protein

Motility protein B

Gene
motB, b1889, JW1878
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processi

  1. archaeal or bacterial-type flagellum-dependent cell motility Source: UniProtKB-KW
  2. chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Motility protein B
Alternative name(s):
Chemotaxis protein MotB
Gene namesi
Name:motB
Ordered Locus Names:b1889, JW1878
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10602. motB.

Subcellular locationi

Cell inner membrane; Single-pass type II membrane protein
Note: The OmpA-like domain probably functions to anchor the complex to the cell wall.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727Cytoplasmic InferredAdd
BLAST
Transmembranei28 – 4922Helical; Signal-anchor for type II membrane protein; InferredAdd
BLAST
Topological domaini50 – 308259Periplasmic InferredAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: EcoliWiki
  2. plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311A → T: Complete loss of motility.
Mutagenesisi32 – 321D → N: Complete loss of motility. 1 Publication
Mutagenesisi39 – 391A → V: Complete loss of motility. 1 Publication
Mutagenesisi159 – 1591P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
Mutagenesisi164 – 1641G → D: Complete loss of motility. 1 Publication
Mutagenesisi196 – 1961T → I: Complete loss of motility. 1 Publication
Mutagenesisi197 – 1971D → N: Complete loss of motility. 1 Publication
Mutagenesisi205 – 2051E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication
Mutagenesisi214 – 2141S → F: Complete loss of motility. 1 Publication
Mutagenesisi217 – 2171R → W: Complete loss of motility. 1 Publication
Mutagenesisi222 – 2221R → H: Complete loss of motility. 1 Publication
Mutagenesisi240 – 2401G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
Mutagenesisi242 – 2421A → T or V: Complete loss of motility. 1 Publication
Mutagenesisi258 – 2581R → C or H: Complete loss of motility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Motility protein BPRO_0000189585Add
BLAST

Proteomic databases

PaxDbiP0AF06.
PRIDEiP0AF06.

Expressioni

Gene expression databases

GenevestigatoriP0AF06.

Interactioni

Subunit structurei

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.

Protein-protein interaction databases

DIPiDIP-47996N.
IntActiP0AF06. 4 interactions.
STRINGi511145.b1889.

Structurei

3D structure databases

ProteinModelPortaliP0AF06.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 268121OmpA-likeAdd
BLAST

Sequence similaritiesi

Belongs to the MotB family.
Contains 1 OmpA-like domain.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1360.
HOGENOMiHOG000254689.
KOiK02557.
OMAiQDGLRIQ.
OrthoDBiEOG6VQPR8.
PhylomeDBiP0AF06.

Family and domain databases

Gene3Di3.30.1330.60. 1 hit.
InterProiIPR025713. MotB_N_dom.
IPR006665. OmpA/MotB_C.
[Graphical view]
PfamiPF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view]
SUPFAMiSSF103088. SSF103088. 1 hit.
PROSITEiPS51123. OMPA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF06-1 [UniParc]FASTAAdd to Basket

« Hide

MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS    50
SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ 100
PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI 150
QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP 200
YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR 250
GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP 300
TMPSAEPR 308
Length:308
Mass (Da):34,186
Last modified:December 20, 2005 - v1
Checksum:i2F20C551C44BD6E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1.
U00096 Genomic DNA. Translation: AAC74959.1.
AP009048 Genomic DNA. Translation: BAA15710.1.
M34669 Genomic DNA. Translation: AAA23572.1.
PIRiA64952. QRECMB.
RefSeqiNP_416403.1. NC_000913.3.
YP_490151.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74959; AAC74959; b1889.
BAA15710; BAA15710; BAA15710.
GeneIDi12932041.
946402.
KEGGiecj:Y75_p1865.
eco:b1889.
PATRICi32119105. VBIEscCol129921_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1 .
U00096 Genomic DNA. Translation: AAC74959.1 .
AP009048 Genomic DNA. Translation: BAA15710.1 .
M34669 Genomic DNA. Translation: AAA23572.1 .
PIRi A64952. QRECMB.
RefSeqi NP_416403.1. NC_000913.3.
YP_490151.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AF06.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47996N.
IntActi P0AF06. 4 interactions.
STRINGi 511145.b1889.

Protein family/group databases

TCDBi 1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Proteomic databases

PaxDbi P0AF06.
PRIDEi P0AF06.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74959 ; AAC74959 ; b1889 .
BAA15710 ; BAA15710 ; BAA15710 .
GeneIDi 12932041.
946402.
KEGGi ecj:Y75_p1865.
eco:b1889.
PATRICi 32119105. VBIEscCol129921_1970.

Organism-specific databases

EchoBASEi EB0597.
EcoGenei EG10602. motB.

Phylogenomic databases

eggNOGi COG1360.
HOGENOMi HOG000254689.
KOi K02557.
OMAi QDGLRIQ.
OrthoDBi EOG6VQPR8.
PhylomeDBi P0AF06.

Enzyme and pathway databases

BioCyci EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Miscellaneous databases

PROi P0AF06.

Gene expression databases

Genevestigatori P0AF06.

Family and domain databases

Gene3Di 3.30.1330.60. 1 hit.
InterProi IPR025713. MotB_N_dom.
IPR006665. OmpA/MotB_C.
[Graphical view ]
Pfami PF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view ]
SUPFAMi SSF103088. SSF103088. 1 hit.
PROSITEi PS51123. OMPA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane."
    Stader J., Matsumura P., Vacante D., Dean G.E., Macnab R.M.
    J. Bacteriol. 166:244-252(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Tandem translation starts in the cheA locus of Escherichia coli."
    Kofoid E.C., Parkinson J.S.
    J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-308.
  6. "Bacterial motility: membrane topology of the Escherichia coli MotB protein."
    Chun S.Y., Parkinson J.S.
    Science 239:276-278(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY, SUGGESTION OF ROLE AS A CELL-WALL ANCHOR.
  7. "Mutant MotB proteins in Escherichia coli."
    Blair D.F., Kim D.Y., Berg H.C.
    J. Bacteriol. 173:4049-4055(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-32; ALA-39; PRO-159; GLY-164; THE-196; ASP-197; GLU-205; SER-214; ARG-217; ARG-222; GLY-240; ALA-242 AND ARG-258.
    Strain: K12 / RP437.
  8. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
    Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
    Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / RP3098.
  9. "Flagellar motility in bacteria structure and function of flagellar motor."
    Terashima H., Kojima S., Homma M.
    Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiMOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AF06
Secondary accession number(s): P09349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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