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Protein

Motility protein B

Gene

motB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processi

  1. archaeal or bacterial-type flagellum-dependent cell motility Source: UniProtKB-KW
  2. chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Motility protein B
Alternative name(s):
Chemotaxis protein MotB
Gene namesi
Name:motB
Ordered Locus Names:b1889, JW1878
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10602. motB.

Subcellular locationi

Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: The OmpA-like domain probably functions to anchor the complex to the cell wall.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727Cytoplasmic1 PublicationAdd
BLAST
Transmembranei28 – 4922Helical; Signal-anchor for type II membrane proteinCuratedAdd
BLAST
Topological domaini50 – 308259Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: EcoliWiki
  2. plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311A → T: Complete loss of motility.
Mutagenesisi32 – 321D → N: Complete loss of motility. 1 Publication
Mutagenesisi39 – 391A → V: Complete loss of motility. 1 Publication
Mutagenesisi159 – 1591P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
Mutagenesisi164 – 1641G → D: Complete loss of motility. 1 Publication
Mutagenesisi196 – 1961T → I: Complete loss of motility. 1 Publication
Mutagenesisi197 – 1971D → N: Complete loss of motility. 1 Publication
Mutagenesisi205 – 2051E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication
Mutagenesisi214 – 2141S → F: Complete loss of motility. 1 Publication
Mutagenesisi217 – 2171R → W: Complete loss of motility. 1 Publication
Mutagenesisi222 – 2221R → H: Complete loss of motility. 1 Publication
Mutagenesisi240 – 2401G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication
Mutagenesisi242 – 2421A → T or V: Complete loss of motility. 1 Publication
Mutagenesisi258 – 2581R → C or H: Complete loss of motility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Motility protein BPRO_0000189585Add
BLAST

Proteomic databases

PaxDbiP0AF06.
PRIDEiP0AF06.

Expressioni

Gene expression databases

GenevestigatoriP0AF06.

Interactioni

Subunit structurei

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.

Protein-protein interaction databases

DIPiDIP-47996N.
IntActiP0AF06. 4 interactions.
STRINGi511145.b1889.

Structurei

3D structure databases

ProteinModelPortaliP0AF06.
SMRiP0AF06. Positions 99-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 268121OmpA-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MotB family.Curated
Contains 1 OmpA-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1360.
HOGENOMiHOG000254689.
InParanoidiP0AF06.
KOiK02557.
OMAiTIKAVPN.
OrthoDBiEOG6VQPR8.
PhylomeDBiP0AF06.

Family and domain databases

Gene3Di3.30.1330.60. 1 hit.
InterProiIPR025713. MotB_N_dom.
IPR006665. OmpA/MotB_C.
[Graphical view]
PfamiPF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view]
SUPFAMiSSF103088. SSF103088. 1 hit.
PROSITEiPS51123. OMPA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AF06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS
60 70 80 90 100
SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ
110 120 130 140 150
PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI
160 170 180 190 200
QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP
210 220 230 240 250
YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR
260 270 280 290 300
GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP

TMPSAEPR
Length:308
Mass (Da):34,186
Last modified:December 19, 2005 - v1
Checksum:i2F20C551C44BD6E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1.
U00096 Genomic DNA. Translation: AAC74959.1.
AP009048 Genomic DNA. Translation: BAA15710.1.
M34669 Genomic DNA. Translation: AAA23572.1.
PIRiA64952. QRECMB.
RefSeqiNP_416403.1. NC_000913.3.
YP_490151.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74959; AAC74959; b1889.
BAA15710; BAA15710; BAA15710.
GeneIDi12932041.
946402.
KEGGiecj:Y75_p1865.
eco:b1889.
PATRICi32119105. VBIEscCol129921_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24178.1.
U00096 Genomic DNA. Translation: AAC74959.1.
AP009048 Genomic DNA. Translation: BAA15710.1.
M34669 Genomic DNA. Translation: AAA23572.1.
PIRiA64952. QRECMB.
RefSeqiNP_416403.1. NC_000913.3.
YP_490151.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0AF06.
SMRiP0AF06. Positions 99-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47996N.
IntActiP0AF06. 4 interactions.
STRINGi511145.b1889.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Proteomic databases

PaxDbiP0AF06.
PRIDEiP0AF06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74959; AAC74959; b1889.
BAA15710; BAA15710; BAA15710.
GeneIDi12932041.
946402.
KEGGiecj:Y75_p1865.
eco:b1889.
PATRICi32119105. VBIEscCol129921_1970.

Organism-specific databases

EchoBASEiEB0597.
EcoGeneiEG10602. motB.

Phylogenomic databases

eggNOGiCOG1360.
HOGENOMiHOG000254689.
InParanoidiP0AF06.
KOiK02557.
OMAiTIKAVPN.
OrthoDBiEOG6VQPR8.
PhylomeDBiP0AF06.

Enzyme and pathway databases

BioCyciEcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1878-MONOMER.

Miscellaneous databases

PROiP0AF06.

Gene expression databases

GenevestigatoriP0AF06.

Family and domain databases

Gene3Di3.30.1330.60. 1 hit.
InterProiIPR025713. MotB_N_dom.
IPR006665. OmpA/MotB_C.
[Graphical view]
PfamiPF13677. MotB_plug. 1 hit.
PF00691. OmpA. 1 hit.
[Graphical view]
SUPFAMiSSF103088. SSF103088. 1 hit.
PROSITEiPS51123. OMPA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane."
    Stader J., Matsumura P., Vacante D., Dean G.E., Macnab R.M.
    J. Bacteriol. 166:244-252(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Tandem translation starts in the cheA locus of Escherichia coli."
    Kofoid E.C., Parkinson J.S.
    J. Bacteriol. 173:2116-2119(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-308.
  6. "Bacterial motility: membrane topology of the Escherichia coli MotB protein."
    Chun S.Y., Parkinson J.S.
    Science 239:276-278(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY, SUGGESTION OF ROLE AS A CELL-WALL ANCHOR.
  7. "Mutant MotB proteins in Escherichia coli."
    Blair D.F., Kim D.Y., Berg H.C.
    J. Bacteriol. 173:4049-4055(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-32; ALA-39; PRO-159; GLY-164; THE-196; ASP-197; GLU-205; SER-214; ARG-217; ARG-222; GLY-240; ALA-242 AND ARG-258.
    Strain: K12 / RP437.
  8. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
    Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
    Mol. Cell 38:128-139(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / RP3098.
  9. "Flagellar motility in bacteria structure and function of flagellar motor."
    Terashima H., Kojima S., Homma M.
    Int. Rev. Cytol. 270:39-85(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiMOTB_ECOLI
AccessioniPrimary (citable) accession number: P0AF06
Secondary accession number(s): P09349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2005
Last sequence update: December 19, 2005
Last modified: January 6, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.