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Protein

Molybdenum cofactor biosynthesis protein B

Gene

moaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in the biosynthesis of molybdopterin. Can bind GTP and has low GTPase activity. Can bind MPT, but has no MPT adenylyl transferase activity.

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110GTPCurated1

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processMolybdenum cofactor biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1501
UniPathwayiUPA00344

Names & Taxonomyi

Protein namesi
Recommended name:
Molybdenum cofactor biosynthesis protein B
Gene namesi
Name:moaB
Synonyms:chlA2
Ordered Locus Names:b0782, JW0765
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11596 moaB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001709702 – 170Molybdenum cofactor biosynthesis protein BAdd BLAST169

Proteomic databases

EPDiP0AEZ9
PaxDbiP0AEZ9
PRIDEiP0AEZ9

2D gel databases

SWISS-2DPAGEiP0AEZ9

Expressioni

Inductioni

By anaerobiosis, repressed by the molybdenum cofactor.

Interactioni

Subunit structurei

Homohexamer. Dimer of trimers.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi425995023 interactors.
IntActiP0AEZ9 4 interactors.
STRINGi316385.ECDH10B_0850

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 18Combined sources7
Helixi24 – 26Combined sources3
Helixi28 – 39Combined sources12
Beta strandi43 – 50Combined sources8
Helixi54 – 66Combined sources13
Beta strandi67 – 69Combined sources3
Beta strandi72 – 77Combined sources6
Beta strandi80 – 82Combined sources3
Helixi87 – 91Combined sources5
Helixi92 – 94Combined sources3
Beta strandi96 – 98Combined sources3
Helixi100 – 114Combined sources15
Helixi115 – 120Combined sources6
Beta strandi124 – 128Combined sources5
Beta strandi131 – 136Combined sources6
Helixi140 – 149Combined sources10
Helixi151 – 155Combined sources5
Helixi165 – 167Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKZX-ray1.60A/B1-170[»]
1R2KX-ray2.10A/B2-170[»]
ProteinModelPortaliP0AEZ9
SMRiP0AEZ9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEZ9

Family & Domainsi

Sequence similaritiesi

Belongs to the MoaB/Mog family.Curated

Phylogenomic databases

eggNOGiENOG4108RIR Bacteria
COG0521 LUCA
HOGENOMiHOG000281102
InParanoidiP0AEZ9
KOiK03638
OMAiGIAPRDT
PhylomeDBiP0AEZ9

Family and domain databases

CDDicd00886 MogA_MoaB, 1 hit
Gene3Di3.40.980.101 hit
InterProiView protein in InterPro
IPR012245 MoaB
IPR036425 MoaB/Mog-like_dom_sf
IPR001453 MoaB/Mog_dom
IPR013484 MoaB_proteobac
IPR008284 MoCF_biosynth_CS
PANTHERiPTHR43232 PTHR43232, 1 hit
PfamiView protein in Pfam
PF00994 MoCF_biosynth, 1 hit
PIRSFiPIRSF006443 MoaB, 1 hit
SMARTiView protein in SMART
SM00852 MoCF_biosynth, 1 hit
SUPFAMiSSF53218 SSF53218, 1 hit
TIGRFAMsiTIGR02667 moaB_proteo, 1 hit
TIGR00177 molyb_syn, 1 hit
PROSITEiView protein in PROSITE
PS01078 MOCF_BIOSYNTHESIS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVSTEFIP TRIAILTVSN RRGEEDDTSG HYLRDSAQEA GHHVVDKAIV
60 70 80 90 100
KENRYAIRAQ VSAWIASDDV QVVLITGGTG LTEGDQAPEA LLPLFDREVE
110 120 130 140 150
GFGEVFRMLS FEEIGTSTLQ SRAVAGVANK TLIFAMPGST KACRTAWENI
160 170
IAPQLDARTR PCNFHPHLKK
Length:170
Mass (Da):18,665
Last modified:January 23, 2007 - v2
Checksum:iC9AA21A7E12E53B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA Translation: CAA49862.1
U00096 Genomic DNA Translation: AAC73869.1
AP009048 Genomic DNA Translation: BAA35440.1
PIRiS34999 S31880
RefSeqiNP_415303.1, NC_000913.3
WP_000084639.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73869; AAC73869; b0782
BAA35440; BAA35440; BAA35440
GeneIDi945396
KEGGiecj:JW0765
eco:b0782
PATRICifig|1411691.4.peg.1496

Similar proteinsi

Entry informationi

Entry nameiMOAB_ECOLI
AccessioniPrimary (citable) accession number: P0AEZ9
Secondary accession number(s): P30746
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome