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Protein

Membrane-bound lytic murein transglycosylase D

Gene

mltD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division (By similarity).By similarity

Caution

Was originally thought to be involved in hexaheme nitrite reductase (cytochrome c552) expression.1 Publication

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125PROSITE-ProRule annotation1

GO - Molecular functioni

  • lyase activity Source: UniProtKB-KW
  • lytic endotransglycosylase activity Source: EcoCyc
  • lytic transglycosylase activity Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan metabolic process Source: InterPro
  • positive regulation of oxidoreductase activity Source: EcoliWiki

Keywordsi

Molecular functionLyase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG10246-MONOMER
MetaCyc:EG10246-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase D (EC:4.2.2.n1)
Alternative name(s):
Murein hydrolase D
Regulatory protein DniR
Gene namesi
Name:mltD
Synonyms:dniR, yafG
Ordered Locus Names:b0211, JW5018
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10246 mltD

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoliWiki
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15PROSITE-ProRule annotationAdd BLAST15
ChainiPRO_000003280116 – 452Membrane-bound lytic murein transglycosylase DAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi16N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi16S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0AEZ7
PRIDEiP0AEZ7

Interactioni

Protein-protein interaction databases

BioGridi4261468, 281 interactors
DIPiDIP-48010N
IntActiP0AEZ7, 5 interactors
STRINGi316385.ECDH10B_0192

Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi401 – 404Combined sources4
Helixi410 – 416Combined sources7
Helixi421 – 427Combined sources7
Helixi431 – 433Combined sources3
Beta strandi438 – 441Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E0GNMR-A398-445[»]
ProteinModelPortaliP0AEZ7
SMRiP0AEZ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEZ7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini341 – 384LysM 1PROSITE-ProRule annotationAdd BLAST44
Domaini400 – 448LysM 2PROSITE-ProRule annotationAdd BLAST49

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 198Slt-type domainAdd BLAST86

Domaini

LysM domains are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the transglycosylase Slt family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105E9H Bacteria
COG0741 LUCA
HOGENOMiHOG000258639
InParanoidiP0AEZ7
KOiK08307
OMAiQDVWERM
PhylomeDBiP0AEZ7

Family and domain databases

CDDicd00118 LysM, 2 hits
Gene3Di3.10.350.10, 2 hits
InterProiView protein in InterPro
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR023346 Lysozyme-like_dom_sf
IPR000189 Transglyc_AS
IPR008258 Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF01476 LysM, 2 hits
PF01464 SLT, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 2 hits
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS51782 LYSM, 2 hits
PS51257 PROKAR_LIPOPROTEIN, 1 hit
PS00922 TRANSGLYCOSYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKAILLAS VLLVGCQSTG NVQQHAQSLS AAGQGEAAKF TSQARWMDDG
60 70 80 90 100
TSIAPDGDLW AFIGDELKMG IPENDRIREQ KQKYLRNKSY LHDVTLRAEP
110 120 130 140 150
YMYWIAGQVK KRNMPMELVL LPIVESAFDP HATSGANAAG IWQIIPSTGR
160 170 180 190 200
NYGLKQTRNY DARRDVVAST TAALNMMQRL NKMFDGDWLL TVAAYNSGEG
210 220 230 240 250
RVMKAIKTNK ARGKSTDFWS LPLPQETKQY VPKMLALSDI LKNSKRYGVR
260 270 280 290 300
LPTTDESRAL ARVHLSSPVE MAKVADMAGI SVSKLKTFNA GVKGSTLGAS
310 320 330 340 350
GPQYVMVPKK HADQLRESLA SGEIAAVQST LVADNTPLNS RVYTVRSGDT
360 370 380 390 400
LSSIASRLGV STKDLQQWNK LRGSKLKPGQ SLTIGAGSSA QRLANNSDSI
410 420 430 440 450
TYRVRKGDSL SSIAKRHGVN IKDVMRWNSD TANLQPGDKL TLFVKNNNMP

DS
Length:452
Mass (Da):49,417
Last modified:December 20, 2005 - v1
Checksum:i80CD5BBE5F5949E8
GO

Sequence cautioni

The sequence CAA43144 differs from that shown. Reason: Frameshift at positions 55, 87 and 226.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69 – 72MGIP → DGNS (PubMed:1663890).Curated4
Sequence conflicti217D → A (PubMed:1663890).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08633.1
U00096 Genomic DNA Translation: AAC73316.1
AP009048 Genomic DNA Translation: BAA77882.2
X60739 Genomic DNA Translation: CAA43144.1 Frameshift.
PIRiE64745
RefSeqiNP_414747.1, NC_000913.3
WP_000644685.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73316; AAC73316; b0211
BAA77882; BAA77882; BAA77882
GeneIDi945694
KEGGiecj:JW5018
eco:b0211
PATRICifig|511145.12.peg.213

Similar proteinsi

Entry informationi

Entry nameiMLTD_ECOLI
AccessioniPrimary (citable) accession number: P0AEZ7
Secondary accession number(s): P23931, P32982, P77350
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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