ID   METF_SHIFL              Reviewed;         296 AA.
AC   P0AEZ2; P00394;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   16-JUN-2009, entry version 30.
DE   RecName: Full=5,10-methylenetetrahydrofolate reductase;
DE            EC=1.5.1.20;
GN   Name=metF; OrderedLocusNames=SF4019, S3728;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   MEDLINE=22272406; PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H.,
RA   Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J.,
RA   Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S.,
RA   Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y.,
RA   Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   MEDLINE=22590274; PubMed=12704152;
RX   DOI=10.1128/IAI.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W.,
RA   Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A.,
RA   Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S.,
RA   Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella
RT   flexneri serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; AE005674; AAN45452.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18748.1; -; Genomic_DNA.
DR   RefSeq; NP_709745.1; -.
DR   RefSeq; NP_838937.1; -.
DR   SMR; P0AEZ2; 3-294.
DR   GeneID; 1027371; -.
DR   GeneID; 1079941; -.
DR   GenomeReviews; AE005674_GR; SF4019.
DR   GenomeReviews; AE014073_GR; S3728.
DR   KEGG; sfl:SF4019; -.
DR   KEGG; sfx:S3728; -.
DR   HOGENOM; P0AEZ2; -.
DR   OMA; P0AEZ2; FFPPKTP.
DR   BioCyc; SFLE198214:AAN45452.1-MON; -.
DR   BRENDA; 1.5.1.20; 189495.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IEA:EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   InterPro; IPR004620; MTHF_reductase_bac.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00676; fadh2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Complete proteome; FAD; Flavoprotein;
KW   Methionine biosynthesis; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    296       5,10-methylenetetrahydrofolate reductase.
FT                                /FTId=PRO_0000190265.
SQ   SEQUENCE   296 AA;  33103 MW;  B702702D2BE9521E CRC64;
     MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY
     GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD
     LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA
     ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD
     GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL
//