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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei88 – 881FADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity
Binding sitei279 – 2791SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi118 – 1203FADBy similarity
Nucleotide bindingi131 – 1322FADBy similarity
Nucleotide bindingi156 – 1594FADBy similarity
Nucleotide bindingi165 – 1728FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:SF4019, S3728
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000002673 Componenti: Chromosome UP000001006 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190265Add
BLAST

Proteomic databases

PRIDEiP0AEZ2.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi198214.SF4019.

Structurei

3D structure databases

ProteinModelPortaliP0AEZ2.
SMRiP0AEZ2. Positions 21-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS
60 70 80 90 100
LKPKFVSVTY GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR
110 120 130 140 150
TIARDYWNNG IRHIVALRGD LPPGSGKPEM YASDLVTLLK EVADFDISVA
160 170 180 190 200
AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS
210 220 230 240 250
AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD GLDDDAETRK
260 270 280 290
LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL
Length:296
Mass (Da):33,103
Last modified:July 21, 1986 - v1
Checksum:iB702702D2BE9521E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45452.1.
AE014073 Genomic DNA. Translation: AAP18748.1.
RefSeqiNP_709745.1. NC_004337.2.
NP_838937.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN45452; AAN45452; SF4019.
AAP18748; AAP18748; S3728.
GeneIDi1027371.
1079941.
KEGGisfl:SF4019.
sfx:S3728.
PATRICi18709290. VBIShiFle31049_4070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45452.1.
AE014073 Genomic DNA. Translation: AAP18748.1.
RefSeqiNP_709745.1. NC_004337.2.
NP_838937.1. NC_004741.1.

3D structure databases

ProteinModelPortaliP0AEZ2.
SMRiP0AEZ2. Positions 21-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF4019.

Proteomic databases

PRIDEiP0AEZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN45452; AAN45452; SF4019.
AAP18748; AAP18748; S3728.
GeneIDi1027371.
1079941.
KEGGisfl:SF4019.
sfx:S3728.
PATRICi18709290. VBIShiFle31049_4070.

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiMETF_SHIFL
AccessioniPrimary (citable) accession number: P0AEZ2
Secondary accession number(s): P00394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.