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P0AEZ2

- METF_SHIFL

UniProt

P0AEZ2 - METF_SHIFL

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Protein

5,10-methylenetetrahydrofolate reductase

Gene
metF, SF4019, S3728
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptor By similarity
Binding sitei88 – 881FAD By similarity
Binding sitei120 – 1201Substrate By similarity
Binding sitei152 – 1521FAD By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei219 – 2191Substrate By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei275 – 2751Substrate By similarity
Binding sitei279 – 2791Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NAD By similarity
Nucleotide bindingi59 – 624FAD By similarity
Nucleotide bindingi59 – 602NAD By similarity
Nucleotide bindingi118 – 1203FAD By similarity
Nucleotide bindingi131 – 1322FAD By similarity
Nucleotide bindingi156 – 1594FAD By similarity
Nucleotide bindingi165 – 1728FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:SF4019, S3728
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190265Add
BLAST

Proteomic databases

PRIDEiP0AEZ2.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi198214.SF4019.

Structurei

3D structure databases

ProteinModelPortaliP0AEZ2.
SMRiP0AEZ2. Positions 21-295.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEZ2-1 [UniParc]FASTAAdd to Basket

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MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS    50
LKPKFVSVTY GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR 100
TIARDYWNNG IRHIVALRGD LPPGSGKPEM YASDLVTLLK EVADFDISVA 150
AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS 200
AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD GLDDDAETRK 250
LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL 296
Length:296
Mass (Da):33,103
Last modified:July 21, 1986 - v1
Checksum:iB702702D2BE9521E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN45452.1.
AE014073 Genomic DNA. Translation: AAP18748.1.
RefSeqiNP_709745.1. NC_004337.2.
NP_838937.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN45452; AAN45452; SF4019.
AAP18748; AAP18748; S3728.
GeneIDi1027371.
1079941.
KEGGisfl:SF4019.
sfx:S3728.
PATRICi18709290. VBIShiFle31049_4070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN45452.1 .
AE014073 Genomic DNA. Translation: AAP18748.1 .
RefSeqi NP_709745.1. NC_004337.2.
NP_838937.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AEZ2.
SMRi P0AEZ2. Positions 21-295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF4019.

Proteomic databases

PRIDEi P0AEZ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN45452 ; AAN45452 ; SF4019 .
AAP18748 ; AAP18748 ; S3728 .
GeneIDi 1027371.
1079941.
KEGGi sfl:SF4019.
sfx:S3728.
PATRICi 18709290. VBIShiFle31049_4070.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246232.
KOi K00297.
OMAi FEFFPTK.
OrthoDBi EOG6D2KVW.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiMETF_SHIFL
AccessioniPrimary (citable) accession number: P0AEZ2
Secondary accession number(s): P00394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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