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P0AEZ2

- METF_SHIFL

UniProt

P0AEZ2 - METF_SHIFL

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei88 – 881FADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity
    Binding sitei279 – 2791SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi118 – 1203FADBy similarity
    Nucleotide bindingi131 – 1322FADBy similarity
    Nucleotide bindingi156 – 1594FADBy similarity
    Nucleotide bindingi165 – 1728FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:SF4019, S3728
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190265Add
    BLAST

    Proteomic databases

    PRIDEiP0AEZ2.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi198214.SF4019.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEZ2.
    SMRiP0AEZ2. Positions 21-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246232.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AEZ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS    50
    LKPKFVSVTY GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR 100
    TIARDYWNNG IRHIVALRGD LPPGSGKPEM YASDLVTLLK EVADFDISVA 150
    AYPEVHPEAK SAQADLLNLK RKVDAGANRA ITQFFFDVES YLRFRDRCVS 200
    AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD GLDDDAETRK 250
    LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL 296
    Length:296
    Mass (Da):33,103
    Last modified:July 21, 1986 - v1
    Checksum:iB702702D2BE9521E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN45452.1.
    AE014073 Genomic DNA. Translation: AAP18748.1.
    RefSeqiNP_709745.1. NC_004337.2.
    NP_838937.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN45452; AAN45452; SF4019.
    AAP18748; AAP18748; S3728.
    GeneIDi1027371.
    1079941.
    KEGGisfl:SF4019.
    sfx:S3728.
    PATRICi18709290. VBIShiFle31049_4070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN45452.1 .
    AE014073 Genomic DNA. Translation: AAP18748.1 .
    RefSeqi NP_709745.1. NC_004337.2.
    NP_838937.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0AEZ2.
    SMRi P0AEZ2. Positions 21-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF4019.

    Proteomic databases

    PRIDEi P0AEZ2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN45452 ; AAN45452 ; SF4019 .
    AAP18748 ; AAP18748 ; S3728 .
    GeneIDi 1027371.
    1079941.
    KEGGi sfl:SF4019.
    sfx:S3728.
    PATRICi 18709290. VBIShiFle31049_4070.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246232.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiMETF_SHIFL
    AccessioniPrimary (citable) accession number: P0AEZ2
    Secondary accession number(s): P00394
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3