5,10-methylenetetrahydrofolate reductase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AEZ1 (METF_ECOLI)

Last modified February 9, 2010. Version 44. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydrofolate reductase
EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	b3941, JW3913

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)⁺ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

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Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 296

296

5,10-methylenetetrahydrofolate reductase

PRO_0000190261

Secondary structure

296

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AEZ1-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B702702D2BE9521E
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FASTA

296

33,103

        10         20         30         40         50         60 
MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY 

        70         80         90        100        110        120 
GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD 

       130        140        150        160        170        180 
LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA 

       190        200        210        220        230        240 
ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD 

       250        260        270        280        290 
GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region." Saint-Girons I., Duchange N., Zakin M.M., Park I., Margarita D., Ferrara P., Cohen G.N. Nucleic Acids Res. 11:6723-6732(1983) [PubMed: 6356036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia." Guenther B.D., Sheppard C.A., Tran P., Rozen R., Matthews R.G., Ludwig M.L. Nat. Struct. Biol. 6:359-365(1999) [PubMed: 10201405] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01502 Genomic DNA. Translation: CAA24747.1.
L19201 Genomic DNA. Translation: AAB03073.1.
U00096 Genomic DNA. Translation: AAC76923.1.
AP009048 Genomic DNA. Translation: BAE77369.1.

PIR

RDECMH. A00462.

RefSeq

AP_003868.1.
NP_418376.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B5T	X-ray	2.50	A/B/C	21-294	[»]
1ZP3	X-ray	1.85	A/B/C	1-296	[»]
1ZP4	X-ray	1.85	A/B/C	1-296	[»]
1ZPT	X-ray	1.95	A/B/C	1-296	[»]
1ZRQ	X-ray	2.20	A/B/C	1-296	[»]
2FMN	X-ray	2.05	A/B/C	1-296	[»]
2FMO	X-ray	2.25	A/B/C	1-296	[»]
3FST	X-ray	1.65	A/C/E	1-296	[»]
3FSU	X-ray	1.70	A/C/E	1-296	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0AEZ1.

Genome annotation databases

GeneID

948432.

GenomeReviews

Gene locus JW3913 in contig AP009048_GR.
Gene locus b3941 in contig U00096_GR.

KEGG

ecj:JW3913.
eco:b3941.

Organism-specific databases

EchoBASE

EB0580.

EcoGene

EG10585. metF.

CMR

Search...

Phylogenomic databases

eggNOG

COG0685.

HOGENOM

HBG649727.

OMA

FFPPKTP.

Enzyme and pathway databases

BioCyc

EcoCyc:METHYLENETHFREDUCT-MONOMER.
ECOL168927:B3941-MONOMER.
MetaCyc:METHYLENETHFREDUCT-MONOMER.

Gene expression databases

Genevestigator

P0AEZ1.

Family and domain databases

InterPro

IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]

Pfam

PF02219. MTHFR. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00676. fadh2. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

METF_ECOLI

Accession

Primary (citable) accession number: P0AEZ1
Secondary accession number(s): P00394, Q2M8N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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