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P0AEZ1 (METF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
Ordered Locus Names:b3941, JW3913
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2962965,10-methylenetetrahydrofolate reductase
PRO_0000190261

Secondary structure

.......................................... 296
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AEZ1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B702702D2BE9521E

FASTA29633,103
        10         20         30         40         50         60 
MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY 

        70         80         90        100        110        120 
GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD 

       130        140        150        160        170        180 
LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA 

       190        200        210        220        230        240 
ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD 

       250        260        270        280        290 
GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region."
Saint-Girons I., Duchange N., Zakin M.M., Park I., Margarita D., Ferrara P., Cohen G.N.
Nucleic Acids Res. 11:6723-6732(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia."
Guenther B.D., Sheppard C.A., Tran P., Rozen R., Matthews R.G., Ludwig M.L.
Nat. Struct. Biol. 6:359-365(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01502 Genomic DNA. Translation: CAA24747.1.
L19201 Genomic DNA. Translation: AAB03073.1.
U00096 Genomic DNA. Translation: AAC76923.1.
AP009048 Genomic DNA. Translation: BAE77369.1.
PIRRDECMH. A00462.
RefSeqNP_418376.1. NC_000913.2.
YP_491510.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B5TX-ray2.50A/B/C21-294[»]
1ZP3X-ray1.85A/B/C1-296[»]
1ZP4X-ray1.85A/B/C1-296[»]
1ZPTX-ray1.95A/B/C1-296[»]
1ZRQX-ray2.20A/B/C1-296[»]
2FMNX-ray2.05A/B/C1-296[»]
2FMOX-ray2.25A/B/C1-296[»]
3FSTX-ray1.65A/C/E1-296[»]
3FSUX-ray1.70A/C/E1-296[»]
ProteinModelPortalP0AEZ1.
SMRP0AEZ1. Positions 21-295.
ModBaseSearch...

Protein-protein interaction databases

IntActP0AEZ1. 4 interactions.
STRING511145.b3941.

Proteomic databases

PaxDbP0AEZ1.
PRIDEP0AEZ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76923; AAC76923; b3941.
BAE77369; BAE77369; BAE77369.
GeneID12934069.
948432.
KEGGecj:Y75_p3246.
eco:b3941.
PATRIC32123403. VBIEscCol129921_4062.

Organism-specific databases

EchoBASEEB0580.
EcoGeneEG10585. metF.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246232.
KOK00297.
OMASITQMFF.
ProtClustDBPRK09432.

Enzyme and pathway databases

BioCycEcoCyc:METHYLENETHFREDUCT-MONOMER.
ECOL316407:JW3913-MONOMER.
MetaCyc:METHYLENETHFREDUCT-MONOMER.
UniPathwayUPA00193.

Gene expression databases

GenevestigatorP0AEZ1.

Family and domain databases

InterProIPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00676. fadh2. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AEZ1.

Entry information

Entry nameMETF_ECOLI
AccessionPrimary (citable) accession number: P0AEZ1
Secondary accession number(s): P00394, Q2M8N7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families