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Protein

Multidrug transporter MdfA

Gene

mdfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na+ or K+.5 Publications

Miscellaneous

A negative charge at position 26 is required for efficient transport of positively charged substrates, but not for neutral compounds. This negative charge does not play an essential role in the multidrug transport mechanism.
Has a sensitive conformational switch that can be triggered either by substrate binding or by attaching unrelated agents inside the pocket. Dissimilar substrates induce similar conformational changes.

Enzyme regulationi

Inhibited by CCCP.1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

GO - Molecular functioni

  • drug transmembrane transporter activity Source: EcoCyc
  • potassium:proton antiporter activity Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoCyc

GO - Biological processi

  • drug transmembrane transport Source: EcoCyc
  • regulation of cellular pH Source: EcoCyc
  • response to antibiotic Source: EcoCyc

Keywordsi

Biological processAntibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CMR-MONOMER
MetaCyc:CMR-MONOMER

Protein family/group databases

TCDBi2.A.1.2.19 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug transporter MdfA
Alternative name(s):
Chloramphenicol resistance pump Cmr
Gene namesi
Name:mdfA
Synonyms:cmlA, cmr
Ordered Locus Names:b0842, JW0826
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13696 mdfA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicCuratedAdd BLAST14
Transmembranei15 – 32HelicalCuratedAdd BLAST18
Topological domaini33 – 53PeriplasmicCuratedAdd BLAST21
Transmembranei54 – 72HelicalCuratedAdd BLAST19
Topological domaini73 – 82CytoplasmicCurated10
Transmembranei83 – 103HelicalCuratedAdd BLAST21
Topological domaini104 – 109PeriplasmicCurated6
Transmembranei110 – 130HelicalCuratedAdd BLAST21
Topological domaini131 – 144CytoplasmicCuratedAdd BLAST14
Transmembranei145 – 165HelicalCuratedAdd BLAST21
Topological domaini166PeriplasmicCurated1
Transmembranei167 – 187HelicalCuratedAdd BLAST21
Topological domaini188 – 226CytoplasmicCuratedAdd BLAST39
Transmembranei227 – 247HelicalCuratedAdd BLAST21
Topological domaini248 – 255PeriplasmicCurated8
Transmembranei256 – 276HelicalCuratedAdd BLAST21
Topological domaini277 – 287CytoplasmicCuratedAdd BLAST11
Transmembranei288 – 308HelicalCuratedAdd BLAST21
Topological domaini309 – 314PeriplasmicCurated6
Transmembranei315 – 335HelicalCuratedAdd BLAST21
Topological domaini336 – 346CytoplasmicCuratedAdd BLAST11
Transmembranei347 – 367HelicalCuratedAdd BLAST21
Topological domaini368 – 378PeriplasmicCuratedAdd BLAST11
Transmembranei379 – 399HelicalCuratedAdd BLAST21
Topological domaini400 – 410CytoplasmicCuratedAdd BLAST11

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26E → A: Loss of ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → D: No change in ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → H: Almost no chloramphenicol efflux activity. Loss of ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → I or V: Slight decrease in chloramphenicol efflux activity. Exhibits low ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → K: Decrease in TPP efflux activity. Exhibits low ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → L or N: Strong decrease in chloramphenicol efflux activity. Loss of ethidium bromide and TPP efflux activities. 1 Publication1
Mutagenesisi26E → Q: Slight decrease in chloramphenicol efflux activity. Loss of ethidium bromide efflux activity. 1 Publication1
Mutagenesisi26E → T: Strong decrease in chloramphenicol efflux activity. Loss of ethidium bromide efflux activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4785

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001733311 – 410Multidrug transporter MdfAAdd BLAST410

Proteomic databases

PaxDbiP0AEY8
PRIDEiP0AEY8

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262828, 108 interactors
DIPiDIP-48116N
IntActiP0AEY8, 1 interactor
STRINGi316385.ECDH10B_0911

Chemistry databases

BindingDBiP0AEY8

Structurei

Secondary structure

1410
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Helixi14 – 16Combined sources3
Helixi17 – 34Combined sources18
Helixi36 – 46Combined sources11
Helixi53 – 65Combined sources13
Helixi69 – 79Combined sources11
Helixi81 – 98Combined sources18
Helixi99 – 101Combined sources3
Helixi105 – 116Combined sources12
Helixi117 – 120Combined sources4
Helixi121 – 124Combined sources4
Helixi126 – 133Combined sources8
Helixi136 – 165Combined sources30
Helixi170 – 191Combined sources22
Helixi205 – 216Combined sources12
Helixi219 – 246Combined sources28
Turni247 – 249Combined sources3
Helixi254 – 278Combined sources25
Helixi279 – 281Combined sources3
Helixi284 – 308Combined sources25
Helixi313 – 339Combined sources27
Helixi346 – 398Combined sources53

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZOWX-ray2.45A10-400[»]
4ZP0X-ray2.00A9-400[»]
4ZP2X-ray2.20A9-400[»]
ProteinModelPortaliP0AEY8
SMRiP0AEY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QF7 Bacteria
ENOG410XT98 LUCA
HOGENOMiHOG000118215
InParanoidiP0AEY8
KOiK08160
OMAiAMLGMIT
PhylomeDBiP0AEY8

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNKLASGAR LGRQALLFPL CLVLYEFSTY IGNDMIQPGM LAVVEQYQAG
60 70 80 90 100
IDWVPTSMTA YLAGGMFLQW LLGPLSDRIG RRPVMLAGVV WFIVTCLAIL
110 120 130 140 150
LAQNIEQFTL LRFLQGISLC FIGAVGYAAI QESFEEAVCI KITALMANVA
160 170 180 190 200
LIAPLLGPLV GAAWIHVLPW EGMFVLFAAL AAISFFGLQR AMPETATRIG
210 220 230 240 250
EKLSLKELGR DYKLVLKNGR FVAGALALGF VSLPLLAWIA QSPIIIITGE
260 270 280 290 300
QLSSYEYGLL QVPIFGALIA GNLLLARLTS RRTVRSLIIM GGWPIMIGLL
310 320 330 340 350
VAAAATVISS HAYLWMTAGL SIYAFGIGLA NAGLVRLTLF ASDMSKGTVS
360 370 380 390 400
AAMGMLQMLI FTVGIEISKH AWLNGGNGLF NLFNLVNGIL WLSLMVIFLK
410
DKQMGNSHEG
Length:410
Mass (Da):44,321
Last modified:December 20, 2005 - v1
Checksum:iD4466EE680095773
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59T → N in CAA69997 (PubMed:9079913).Curated1
Sequence conflicti225 – 240ALALG…LAWIA → GAGAADSLVCRCWRGSP in AAC44147 (PubMed:8655497).CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44900 Genomic DNA Translation: AAC44147.1
Y08743 Genomic DNA Translation: CAA69997.1
U00096 Genomic DNA Translation: AAC73929.1
AP009048 Genomic DNA Translation: BAA35546.1
PIRiB64822
RefSeqiNP_415363.1, NC_000913.3
WP_001180089.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73929; AAC73929; b0842
BAA35546; BAA35546; BAA35546
GeneIDi945448
KEGGiag:CAA69997
ecj:JW0826
eco:b0842
PATRICifig|1411691.4.peg.1436

Similar proteinsi

Entry informationi

Entry nameiMDFA_ECOLI
AccessioniPrimary (citable) accession number: P0AEY8
Secondary accession number(s): P71226, P75807, Q46966
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health